1
tesis doctoral
Publicado 2014
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Analizar los resultados de eficacia, seguridad y racionalidad de la combinación a dosis fija (CDF) de dos hipoglicemiantes orales (metformina + glimepirida) en publicaciones biomédicas, 2000-2012. Método: Se usó un estudio cuantitativo, revisión documental y retrospectivo, en el cual la unidad de análisis y la muestra fueron los estudios clínicos controlados aleatorizados (ECCA) y número total de publicaciones biomédicas desde el 2000 – 2012 respectivamente. Se realizó la búsqueda en bases de datos de evidencia científica: pubmed, EMBASE, Lilacs, “The ACP Journal Club”, “Bandolier”, “BMJ Evidence Updates”, “The Cochrane Database of Systematic Reviews”, etc, según los recomendado por el Centro de Medicina Basada en la Evidencia (MBE) de la “Duke University Medical Center Library” y Oxford University. Los criterios para evaluar la eficacia, seguridad y ra...
2
artículo
Publicado 2021
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During the race for the development of a vaccine against COVID-19, even before itscommercialization, part of the population has already shown a growing fear of its application. Wedesigned an analytical cross-sectional study using an anonymous survey in the 25 departmentsof Peru. We surveyed whether the participants were planning on getting vaccinated, as well asother characteristics that were cross-checked in a uni-, bi- and multivariate manner. Of the 1776respondents, 70% (1251) stated that they were planning to be vaccinated, 20% (346) did not knowyet or doubted it, and 10% (179) did not want to be vaccinated. We observed that those who did notget infected with COVID-19 exhibited a higher frequency to not wanting or were uncertain aboutgetting vaccinated (aPR: 1.40; 95% CI: 1.09–1.81;p-value = 0.008). In contrast, there was a lowerfrequency of vaccine refusal among university student...
3
artículo
Publicado 2021
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During the race for the development of a vaccine against COVID-19, even before itscommercialization, part of the population has already shown a growing fear of its application. Wedesigned an analytical cross-sectional study using an anonymous survey in the 25 departmentsof Peru. We surveyed whether the participants were planning on getting vaccinated, as well asother characteristics that were cross-checked in a uni-, bi- and multivariate manner. Of the 1776respondents, 70% (1251) stated that they were planning to be vaccinated, 20% (346) did not knowyet or doubted it, and 10% (179) did not want to be vaccinated. We observed that those who did notget infected with COVID-19 exhibited a higher frequency to not wanting or were uncertain aboutgetting vaccinated (aPR: 1.40; 95% CI: 1.09–1.81;p-value = 0.008). In contrast, there was a lowerfrequency of vaccine refusal among university student...
4
artículo
Publicado 2017
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Objetivo. Determinar si la administración crónica (90 días) del látex liofilizado de Croton lechleri Muell. Arg. “sangre de drago” modifica los parámetros hematológicos y bioquimicos en Rattus norvegicusvar albinus. Materiales y métodos. Se utilizó ratas con un peso corporal (p.c.) entre 150 g a 170 g, distribuidos en Grupo A (control) y Grupos B y C (experimentales), de 20 especímenes cada uno (10 hembras y 10 machos) a las que se administró por vía oral NaCl 0,9% y una dosis diaria de 100 y 200 mg de látex liofilizado/kg p.c. respectivamente, durante 90 días. Se tomaron muestras de sangre cada 15 días para determinar parámetros hematológicos (hematocrito, linfocitos, leucocitos y segmentados) y bioquímicos (glucosa, úrea, creatinina, colesterol total y perfil hepático). Resultados. Todos los valores se encontraron dentro del rango normal. Se hallaron diferencias ...
5
artículo
Publicado 2017
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Aims: Cancer is the first cause of death in the Peruvian population; searching alternative treatments of medicinal plants constitute a promissory field to find new anticancer drugs. The main objective in this study was to evaluate the phytochemical screening, total phenolic content, antioxidant and cytotoxic activity of ethanol extract of Chromolaena laevigata (C. laevigata) on human tumor cell lines. Study Design: The fresh leaves of C. laevigata were soaked with ethanol followed by phytochemical screening using standard methods. Place and Duration of Study: Laboratory of Applied Chemistry, Faculty of Pharmacy and Biochemistry, Universidad Nacional San Luis Gonzaga de Ica, Ica, Peru; Laboratory “Abraham Vaisberg Wolach”, Universidad Peruana Cayetano Heredia, Lima, Peru. Methodology: Phytochemical screening was assessed by using chemical reactives. Total phenolic content (TPC) was de...
6
artículo
Publicado 2019
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The authors thank Universidad Nacional Mayor de San Marcos for helping to support this research. Cesar Franco-Quino is a doctoral student studying an Epidemiological Researcher Doctorate at Universidad Peruana Cayetano Heredia under FONDECYT/ CIENCIAACTIVA scholarship EF033-235-2015 and supported by training grant D43 TW007393 awarded by Fogarti International Center of the US National Institutes of Health.
7
artículo
Preventive effect of Oenothera rosea on N-methyl-N-nitrosourea- (NMU) induced gastric cancer in rats
Publicado 2017
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Background: Currently, gastric cancer (GC) is considered a public health problem worldwide. Using medicinal plants for the prevention of chronic diseases such as cancer constitutes new alternatives in traditional medicine. Oenothera rosea (OR) could be an option, but it needs to be evaluated. Aim: The main objective of this study was to evaluate the protective effect of OR extract on N-methyl-N-nitrosourea (NMU)-induced GC in rats. Methods: In total, 80 male Holtzman rats were randomized into five groups. Group A received the saline solution (5mL/kg), group B received NMU 500 μg/kg (cancer inductor) by oral administration for 16 weeks, and groups C, D, and E were treated with OR extract (100, 200, and 300 mg/kg, respectively) and NMU in order to evaluate the preventive effect on cancer induced by NMU for 16 weeks. Blood and histological samples of stomachs were collected to determine hi...
8
artículo
Publicado 2020
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Objective: To determine the ameliorative effect of the ethanolic extract of Chuquiraga spinosa (ChS) on 7,12-Dimethylbenz[a]anthracene (DMBA)-induced breast cancer in rats. Methods: 36 female Holztman rats were divided into 6 groups. I) The negative control group received physiological saline (PS). II) ChS-200 group received 200 mg/kg of ChS. III) DMBA group was induced with DMBA (20 mg/Kg) dissolved in PS and administrated orally for 15 weeks. IV) DMBA + ChS-50 group, V) DMBA + ChS-250 group, and VI) DMBA + ChS-500 group, which received the extract orally for 15 weeks after DMBA induction. All data were expressed as mean and standard deviation. One-way analysis of variance (ANOVA) followed by Dunnet test was carried out to compare the mean value of different groups Histopathological analysis was evaluated by using Image J software. Results: Hematology showed that the triglyceride level ...
9
artículo
Publicado 2020
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Objective: To determine the ameliorative effect of the ethanolic extract of Chuquiraga spinosa (ChS) on 7,12-Dimethylbenz[a]anthracene (DMBA)-induced breast cancer in rats. Methods: 36 female Holztman rats were divided into 6 groups. I) The negative control group received physiological saline (PS). II) ChS-200 group received 200 mg/kg of ChS. III) DMBA group was induced with DMBA (20 mg/Kg) dissolved in PS and administrated orally for 15 weeks. IV) DMBA + ChS-50 group, V) DMBA + ChS-250 group, and VI) DMBA + ChS-500 group, which received the extract orally for 15 weeks after DMBA induction. All data were expressed as mean and standard deviation. One-way analysis of variance (ANOVA) followed by Dunnet test was carried out to compare the mean value of different groups Histopathological analysis was evaluated by using Image J software. Results: Hematology showed that the triglyceride level ...
10
informe técnico
Metodología de la Investigación es un curso específico de la Carrera de Medicina, de carácter teórico-práctico, dirigido a los estudiantes del 7mo ciclo. Se busca que los estudiantes adquieran las competencias generales de razonamiento cuantitativo, manejo de la información y pensamiento innovador, a nivel intermedio; asimismo, se busca adquirir la competencia específica de investigación, a nivel básico. El presente curso inicia al estudiante de Medicina en el proceso de la investigación científica. De manera muy amigable, busca despertar en el alumno su espíritu investigador y su afán por comunicar sus hallazgos. Además, brinda las herramientas estadísticas necesarias para la elaboración de proyectos de investigación, así como el análisis e interpretación de artículos publicados en la literatura científica. El curso es parte de la línea de cursos de investigació...
11
artículo
Publicado 2022
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Schinus molle is a medicinal plant used as an anti-inflammatory and for rheumatic pain in the traditional medicine of Peru. On the other hand, Aedes aegypti is the main vector of several tropical diseases and the transmitter of yellow fever, chikungunya, malaria, dengue, and Zika virus. In this study, the aim was to investigate the antioxidant activity in vitro and the insecticidal activity in silico, in the presence of the mosquito juvenile hormone-binding protein (mJHBP) from Aedes aegypti, of the essential oil from S. molle leaves. The volatile phytochemicals were analyzed by gas chromatography-mass spectrometry (GC-MS), and the profile antioxidants were examined by DPPH, ABTS, and FRAP assays. The evaluation in silico was carried out on mJHBP (PDB: 5V13) with an insecticidal approach. The results revealed that EO presented as the main volatile components to alpha-phellandrene (32.68%...
12
artículo
Publicado 2020
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Carvacrol is a phenol monoterpene found in aromatic plants specially in Lamiaceae family, which has been evaluated in an experimental model of breast cancer. However, any proposed mechanism based on its antitumor effect has not been reported. In our previous study, carvacrol showed a protective effect on 7,12-dimethylbenz[α]anthracene- (DMBA-) induced breast cancer in female rats. The main objective in this research was to evaluate by using in silico study the carvacrol on HER2, PI3Kα, mTOR, hERα, PR, and EGFR receptors involved in breast cancer progression by docking analysis, molecular dynamic, and drug-likeness evaluation. A multilevel computational study to evaluate the antitumor potential of carvacrol focusing on the main targets involved in the breast cancer was carried out. The in silico study starts with protein-ligand docking of carvacrol followed by ligand pathway calculatio...
13
informe técnico
Metodología de la Investigación es un curso específico de la Carrera de Medicina, de carácter teórico-práctico, dirigido a los estudiantes del 7mo ciclo. Se busca que los estudiantes adquieran las competencias generales de razonamiento cuantitativo, manejo de la información y pensamiento innovador, a nivel intermedio; asimismo, se busca adquirir la competencia específica de investigación, a nivel básico. El presente curso inicia al estudiante de Medicina en el proceso de la investigación científica. De manera muy amigable, busca despertar en el alumno su espíritu investigador y su afán por comunicar sus hallazgos. Además, brinda las herramientas estadísticas necesarias para la elaboración de proyectos de investigación, así como el análisis e interpretación de artículos publicados en la literatura científica. El curso es parte de la línea de cursos de investigació...
14
informe técnico
1Metodología de la Investigación es un curso específico de la Carrera de Medicina, de carácter teórico-práctico, dirigido a los estudiantes del 7mo ciclo. Se busca que los estudiantes adquieran las competencias generales de razonamiento cuantitativo, manejo de la información y pensamiento innovador, a nivel intermedio; asimismo, se busca adquirir la competencia específica de investigación, a nivel básico. El presente curso inicia al estudiante de Medicina en el proceso de la investigación científica. De manera muy amigable, busca despertar en el alumno su espíritu investigador y su afán por comunicar sus hallazgos. Además, brinda las herramientas estadísticas necesarias para la elaboración de proyectos de investigación, así como el análisis e interpretación de artículos publicados en la literatura científica. El curso es parte de la línea de cursos de investigaci...
15
informe técnico
Publicado 2023
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La asignatura se desarrolla a través de talleres en los cuales los estudiantes desarrollan un protocolo de investigación que servirá para desarrollar su tesis de titulación como médico cirujano. El protocolo responde a una pregunta de investigación relevante que lleva al desarrollo de una investigación con un diseño metodológico apropiado y con un nivel de calidad potencialmente publicable. Este protocolo será presentado ante un comité de ética en investigación para su revisión.
