Objective: To measure the protective barriers use frequency by undergraduate Medical Technology School students during their elective period at both the University and the hospital. Design: Survey study. Setting: Daniel A. Carrion Tropical Medicine Institute, San Marcos Major National University. Participants: Technology Professional Academic School students. Interventions: A self-administered, anonymous questionnaire covering sex, age, and protective barriers was distributed to 68 fourth- and fifthyear medical technician students at the University of San Marcos, School of Medical Technology, Faculty of Medicine. Main outcome measures: Gown, gloves, pipete pumps and mask use. Results: Sixty-five students (95,5%) returned the questionnaire. The use of gowns, gloves, pipette pumps, and masks was 98,5%, 49,2%, 33,8% and 9,2% at the University, and 78,9%, 78,9%, 52,6% and 26,3% in the hospit...

We present the first case of application of therapeutic hypothermia in a newborn with a diagnosis of moderate ischemic hypoxic encephalopathy with the inclusion criterias. Risk factors, diagnostic criteria, evolution and monitoring during hypothermia and the followed-up post-discharge, are reported in our institution.

We present the first case of application of therapeutic hypothermia in a newborn with a diagnosis of moderate ischemic hypoxic encephalopathy with the inclusion criterias. Risk factors, diagnostic criteria, evolution and monitoring during hypothermia and the followed-up post-discharge, are reported in our institution.

Objective: To determine the prevalence of human fasciolosis in an endemic area by means of an enzyme-linked immunosorbent assay (ELISA) using cystatin as a capture agent for the detection of specific antibodies to Fasciola hepatica cysteine proteinases. Material and methods: An ELISA plate was sensitized with cystatin, incubated with excretory-secretory products of adult flukes, and followed by standard ELISA procedures. Clinical applicability of the cystatin capture ELISA for the immunodiagnosis of fasciolosis was tested with 200 serum samples of children and adults from an endemic area in Chupaca province, Junin department. Results: Serum samples from the endemic area tested by cystatin capture ELISA showed 27/ 200 (13,5%) of positive cases. Conclusions: Fasciolosis remains a major health problem at Chupaca province, Junin department.

Background: Paragonimiasis is a lung disease caused by Paragonimus trematodes genus. Human infection laboratory diagnosis is usually done by detection of parasite eggs in sputum or feces; however, findings may be negative and alternative diagnostic methods are required. Objectives: To compare three serologic tests for detection of Paragonimus mexicanus somatic antigen (PmAS) antibodies in serum samples of cats infected experimentally. Design: Experimental study. Setting: Instituto de Medicina Tropical “Daniel A. Carrión” bioterium, Faculty of Medicine, Universidad Nacional Mayor de San Marcos, Lima, Peru. Biologic material: Domestic cats 4-6 years old and P. mexicanus (PmAS) crude somatic antigen. Interventions: Blood samples were collected from twelve domestic cats infected with P. mexicanus. Serum samples were evaluated by enzyme-linked immunosorbent assay (ELISA), double diffusio...

ABSTRACTMaple syrup urine disease is a congenital metabolic disorder, commonly presenting as acute neonatal encephalopathy.Case 1. A 7-day-old neonate exhibited somnolence and refusal to breastfeed, later developing respiratory failure, neurological impairment, metabolic acidosis, and death. Elevated levels of branched-chain amino acids and a confirmatorygenetic test established the diagnosis. Case 2. A 10-day-old neonate was admitted with breastfeeding refusal, hypoglycemia, and seizures. Elevated branched-chain amino acids confirmed the diagnosis genetically. The patient receivedparenteral nutrition and a special formula, leading to progressive neurological improvement. Currently, at 2 years and 7months, the patient remains under multidisciplinary follow-up. Conclusion. Early detection of maple syrup urine diseasethrough neonatal screening significantly improves neurological outcomes.�...

ABSTRACTMaple syrup urine disease is a congenital metabolic disorder, commonly presenting as acute neonatal encephalopathy.Case 1. A 7-day-old neonate exhibited somnolence and refusal to breastfeed, later developing respiratory failure, neurological impairment, metabolic acidosis, and death. Elevated levels of branched-chain amino acids and a confirmatorygenetic test established the diagnosis. Case 2. A 10-day-old neonate was admitted with breastfeeding refusal, hypoglycemia, and seizures. Elevated branched-chain amino acids confirmed the diagnosis genetically. The patient receivedparenteral nutrition and a special formula, leading to progressive neurological improvement. Currently, at 2 years and 7months, the patient remains under multidisciplinary follow-up. Conclusion. Early detection of maple syrup urine diseasethrough neonatal screening significantly improves neurological outcomes.�...

OBJECTIVES: To assess the prevalence of E. histolytica and E. dispar in a marginal population of Callao, Lima, using ELISAs. MATERIAL AND METHODS: Two ELISAs were used, one of them for both Entamoeba species (Entamoeba-ELISA), and an E. histolytica-specific ELISA. RESULTS: 128 stool samples from randomized Callao inhabitants were microscopically examined. Thirteen (10%) were microscopically diagnosed as having E. histolytica or E. dispar infection; and 7 (6%) were positive to Entamoeba–ELISA (sensitivity = 54%). Five of the 115 samples without cysts of E. histolytica or E. dispar were Entamoeba ELISA positive (specificity = 96%). All samples were negative to the E. histolytica-specific ELISA. CONCLUSIONS: The correlation between the microscopical identification and Entamoeba-ELISA was not good, it may be due to an overvaluation of the morphological diagnosis.

Objetive: To standardise ELISA technique for Toxocara canis human infection diagnosis by using excreted-secreted antigen prepared in our country. Material and methods: T. canis eggs were collected by incubation with formalin (2%) at 28oC in order to obtain third stage larvae that were freed and incubated in RPMI at 37°C for 7 days; the medium was replaced by a similar one and stored at - 20°C. Antigen was concentrated and protein dosage was made. Sera from patients with toxocariasis and newborns were used as positive and negative controls by ELISA technique, dilutions ¼ to 1/1024. Polystyrene plates were sensitised with antigen in several concentrations and conjugated peroxidase with horseradish IgG, anti human IgG and substrate OPD were used. Absorbance was read with spectrophotometer (Multiskan plus labsystems) at 492 nm. Cut off point was determined by negative sera absorbencies ar...

Objective: To estimate intestinal coccidiae infection prevalence of in asymptomatic children from a marginal community in Lima. Material and Methods: Stool samples were collected from 79 asymptomatic children aged 7 months through 7 year-old from Asentamiento Humano “Las Casuarinas de Villa”. Direct smear and colored frotis by Kinyoun technique were prepared. Another portion was placed in small bottles with potasium bicromatum 2,5% to obtain sporulation. After 10 days, frotis were colored by Kinyoun. Results: Direct smear detected one sample with Cryptosporidium (1,3%). In frotis colored before sporulation, 3 with Cryptosporidium (3,8%) were found and one with Isospora belli (1,3%); in frotis colored post-sporulation 6 samples with Cryptosporidium (7.59%) were detected and one with Isospora belli. Conclusion: Sporulation increases Cryptosporidium detection.

El objetivo del estudio fue analizar el uso de la punción lumbar (PL) en las sospechas de sepsis neonatal tardía. Se utilizó una cohorte de 414 neonatos con peso al nacer <2000 g en tres hospitales de Lima. Se realizó la PL en 45/214 (21,0%) sospechas de sepsis y en 13/48 (27,1%) sepsis confirmadas por hemocultivo. Se diagnosticó meningitis en 8/214 (3,7%) sospechas y en 8/45 (17,5%) episodios en los que se realizó la PL. El tiempo de tratamiento de los episodios sin PL fue similar a los episodios de sepsis con meningitis descartada y menor a los episodios de meningitis. El uso de la PL es bajo, lo que puede resultar en meningitis no diagnosticadas y tratadas inadecuadamente.

Objetivos: Caracterizar las moléculas de respuesta humoral anti Paragonimus mexicanus/P. peruvianus para diferenciar la infección en fase aguda y fase crónica, mediante la evaluación de la dinámica de la respuesta inmune. Diseño: Observacional, longitudinal. Institución: Instituto de Medicina Tropical Daniel A. Carrión, Facultad de Medicina, UNMSM Material biológico: Animales de experimentación. Principales medidas de resultados: Determinación de IgG e IgM anti Paragonimus. Resultados: Existió diferencia significativa de medias de la DO entre los grupos crónico y no infectados para los anticuerpos IgG anti ES de Paragonimus. Los anticuerpos IgM anti Paragonimus tuvieron una ligera elevación entre los días 7 a 20 post inoculación (pi), y una tendencia hacia la disminución entre los días 40 y 60 pi. Conclusiones: La dinámica de producción de anticuerpos IgG e IgM anti P...

We report the first successful case of fetal surgery for spina bifida repair in Peru. A pregnant woman was referred to our center at 23 weeks’ gestation because of prenatal diagnosis of spina bifida. We formed a multidisciplinary international team with the goal of performing the first open intrauterine surgery in Peru. We performed a successful intrauterine surgery at 25 weeks of gestation and a healthy infant was born by cesarean section at 37 weeks of gestation. We demonstrated the feasibility of this complex intrauterine surgery in our local setting.