1
artículo
Publicado 2002
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Objective: To determine the presence of Acanthamoeba genus in symptomatic persons. Setting: Cayetano Heredia National Hospital Ophthalmology Service. Material and methods: One hundred individuals without evidence of corneal lesion had corneal samples examined during April and May 1999. Samples were sowed in neapolitan agar and non-nutritive agar, incubated and checked after 7 days. Results: Acanthamoeba cysts were found in 16 out of the 100 samples; 44 subjects were male; diagnosis was not different in both sexes; 63% of individuals were below 20 year-old; infection was predominant in younger people; 89% referred visits to areas where the parasite had been reported (Piura, Ica); 58 subjects referred swimming in pools or rivers. Out of 76 subjects who reported daily use water source, 63% obtained it from water-tank cars and 25% from wells; infection was more frequent in the latter ones (3...
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artículo
OBJECTIVE: To assess the prevalence of paragonimosis among elementary school students from three villages of Condebamba valley, Cajamarca - Perú. MATERIAL AND METHODS: 77 school-age children underwent parasitologic examination in stools, skin test and counterimmunoelectrophoresis in those positive for skin reactivity. RESULTS: From 77 skin tests performed, 2 were positive (2,6%), which were also positive by counterimmunoelectrophoresis. All 77 stool samples were negative for Paragonimus eggs. CONCLUSION: These results showed a decreasing prevalence of human paragonimiasis among Condebamba valley children.
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artículo
Objective: To estimate seroprevalence of human toxocariosis in Lima inhabitants. Design: Cross-sectional study, non aleatory selection. Material and methods: To people living in Lima city urban marginal communities, an interview and clinical examination were done and serum samples obtained to detect antibodies against Toxocara by ELISA technique. Stool samples were also obtained to check parasites causing cross reactions with serology. Results: From 553 persons examined 23,3% were reactive. There were no sex differences and ELISA results related to anemia, leucocytosis, eosinophilia or intestinal parasites. People older than 15 yearold had twice the possibility to be reactive. Most frequent symptom in positive individuals was decrease of visual capacity. Most frequent parasites were Blastocystis hominis, Entamoeba coli, Giardia lamblia. Conclusions: Human toxocariosis is frequent in Lima...
4
artículo
Publicado 2009
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Se evaluó una prueba de aglutinación de látex estandarizada para el diagnóstico serológico de la echinococosis quística en Perú. Se utilizó partículas de látex de 0,25 um de diámetro, las cuales fueron sensibilizadas con antígeno total de líquido liofilizado de quiste hidatídico de ovino. Se usaron 80 sueros, 40 de pacientes operados por enfermedad hidatídica confirmada patología, 20 de pacientes con otras enfermedades parasitarias como cisticercosis, fasciolosis, toxoplasmosis, teniosis, enfermedad de chagas y malaria y 20 de personas sanas. Se encontró que de los 40 sueros de pacientes con hidatidosis uno dio resultado negativo y de los 40 sueros de pacientes o personas sin hidatidosis cuatro dieron resultados positivo obteniéndose una sensibilidad de 97,5%, una especificidad de 90,0%, un valor predictivo positivo de 90,7% y negativo de 97,3%, siendo la concordancia e...
5
artículo
Publicado 1999
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OBJECTIVES: To assess the prevalence of E. histolytica and E. dispar in a marginal population of Callao, Lima, using ELISAs. MATERIAL AND METHODS: Two ELISAs were used, one of them for both Entamoeba species (Entamoeba-ELISA), and an E. histolytica-specific ELISA. RESULTS: 128 stool samples from randomized Callao inhabitants were microscopically examined. Thirteen (10%) were microscopically diagnosed as having E. histolytica or E. dispar infection; and 7 (6%) were positive to Entamoeba–ELISA (sensitivity = 54%). Five of the 115 samples without cysts of E. histolytica or E. dispar were Entamoeba ELISA positive (specificity = 96%). All samples were negative to the E. histolytica-specific ELISA. CONCLUSIONS: The correlation between the microscopical identification and Entamoeba-ELISA was not good, it may be due to an overvaluation of the morphological diagnosis.
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artículo
Objetive: To standardise ELISA technique for Toxocara canis human infection diagnosis by using excreted-secreted antigen prepared in our country. Material and methods: T. canis eggs were collected by incubation with formalin (2%) at 28oC in order to obtain third stage larvae that were freed and incubated in RPMI at 37°C for 7 days; the medium was replaced by a similar one and stored at - 20°C. Antigen was concentrated and protein dosage was made. Sera from patients with toxocariasis and newborns were used as positive and negative controls by ELISA technique, dilutions ¼ to 1/1024. Polystyrene plates were sensitised with antigen in several concentrations and conjugated peroxidase with horseradish IgG, anti human IgG and substrate OPD were used. Absorbance was read with spectrophotometer (Multiskan plus labsystems) at 492 nm. Cut off point was determined by negative sera absorbencies ar...
7
artículo
Publicado 2010
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ntroduction: There is little and not recent information on the presence of triatomine bugs in San Martin department, Amazon area of our country where Chagas disease has been reported by wild triatomine insects as vectors. Objectives: To determine the geographic distribution of hematophagous triatomine insects in Huallaga and Picota provinces, San Martin department, Peru. To determine the domiciliary infestation by triatomine vectors indexes, trypano-triatomine infection as well as their dispersion. To determine the insects feeding source and to genetically characterize the tripanosomatidae they carry. Design: Observational and descriptive study. Setting: Health and Laboratories region networks. National Institute of Health laboratories. Participants: Houses from 6 districts of Huallaga province and 9 from Picota province (San Martin department). Interventions: Triatomine insect’s colle...
8
artículo
Publicado 2011
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OBJETIVOConocer la prevalencia e infección por enteroparásitos, así como determinar el estado nutricional de una población escolar infantil aparentemente sana de la Institución Educativa Nacional “Karol Wojtyla”, del distrito de San Juan de Lurigancho, Lima-Perú.MATERIAL Y MÉTODO:205 niños, de ambos sexos, entre 6 y 12 años de primer a sexto grado de primaria. Las muestras fueron analizadas utilizando la técnica de sedimentación espontánea (TSET) y el método de Graham.RESULTADOSEn el 44.4 % (91/205) se realizó el examen parasitológico. La prevalencia de enteroparásitos fue 61.50% (56/91), hallando Enterobius vermicularis (14.30%), Hymenolepis nana (8.80%), Blastocystis hominis (38.50%), y Giardia lamblia (13.20%) y no patógenos como Entamoeba coli (17.60%).CONCLUSIONESExiste una alta prevalencia de parasitosis en la población escolar analizada, la que estu...
