Seven third stage nymphs of Triatoma infestans were infected with Trypanosoma cruzi of mice during the acute phase. After a time of being positives, the triatomids became a microscopical negative in feces examination. The feces of these negative triatomids were inoculated to eight mice and controled from 16 to 44 days. Three mice (37,5%) showed infection of the blood. These results suggest the necessity to induce an infection in a mouse upon which, from the fifteenth day on, tests should be conducted in order to check for trypomastigotes.

Seven third stage nymphs of Triatoma infestans were infected with Trypanosoma cruzi of mice during the acute phase. After a time of being positives, the triatomids became a microscopical negative in feces examination. The feces of these negative triatomids were inoculated to eight mice and controled from 16 to 44 days. Three mice (37,5%) showed infection of the blood. These results suggest the necessity to induce an infection in a mouse upon which, from the fifteenth day on, tests should be conducted in order to check for trypomastigotes.

Objective: To determine the presence of Acanthamoeba genus in symptomatic persons. Setting: Cayetano Heredia National Hospital Ophthalmology Service. Material and methods: One hundred individuals without evidence of corneal lesion had corneal samples examined during April and May 1999. Samples were sowed in neapolitan agar and non-nutritive agar, incubated and checked after 7 days. Results: Acanthamoeba cysts were found in 16 out of the 100 samples; 44 subjects were male; diagnosis was not different in both sexes; 63% of individuals were below 20 year-old; infection was predominant in younger people; 89% referred visits to areas where the parasite had been reported (Piura, Ica); 58 subjects referred swimming in pools or rivers. Out of 76 subjects who reported daily use water source, 63% obtained it from water-tank cars and 25% from wells; infection was more frequent in the latter ones (3...

Una muestra de 206 niños (entre 1 y 16 años de edad) en los distritos de Huertas (n=110) y Julcan (n=96) pertenecientes al Valle del Mantaro, Perú, fueron estudiados mediante una entrevista clínico-epidemiológica, pruebas serológicas y exámenes parasitológicos de heces para describir la prevalencia y analizar los factores de riesgo para la infección por Fasciola hepática (Fh). La prevalencia de Fh por exámenes de heces en Huertas (H) fue 28.3% y Julcan (J), 12.6%; empleando pruebas serológicas, las prevalencias fueron: para H, 36.3% y para J, 22.7%. El análisis univariado demostró una asociación significativa entre la infección por Fh y 3 variables en H: vivir cerca de acequias (OR=4.8; P<0.05) o cultivos (OR=2.88; P<0.05) y el hábito de beber emolientes (OR=2.92; P<0.05); y otras 3 variables en J: tener unahabitación por casa (OR=21.0; P<0.05), eli...