The purpose of this study was to assess whether the loss of fur and changes in liver enzymes, aspartate aminotransferase (AST) and alanine aminotransferase (ALT), produced in albino mice by oral administration of carbon tetrachloride and dimethyl sulfoxide (DMSO) (1:1), 2 mL/kg dose, are reversible processes when administered modified aloe gel (GSM) orally daily dose of 8 mL/kg of weight. Tests carried out at 13, 18 and 33 days from starting treatment with GSM, was evidenced significant and progressive decrease in AST and ALT without significant variations in proteins and phosphatases; whereas triglyceride level by the effect of GSM (group B), experienced significant decrease during the first week compared to mice given CCl4+DMSO mixture (group A), specimens that also recovered the fur on its chest and muzzle at 33 days of starting the experiment. The mice in the groups: control, GSM, DM...

The purpose of this study was to assess whether the loss of fur and changes in liver enzymes, aspartate aminotransferase (AST) and alanine aminotransferase (ALT), produced in albino mice by oral administration of carbon tetrachloride and dimethyl sulfoxide (DMSO) (1:1), 2 mL/kg dose, are reversible processes when administered modified aloe gel (GSM) orally daily dose of 8 mL/kg of weight. Tests carried out at 13, 18 and 33 days from starting treatment with GSM, was evidenced significant and progressive decrease in AST and ALT without significant variations in proteins and phosphatases; whereas triglyceride level by the effect of GSM (group B), experienced significant decrease during the first week compared to mice given CCl4+DMSO mixture (group A), specimens that also recovered the fur on its chest and muzzle at 33 days of starting the experiment. The mice in the groups: control, GSM, DM...

The objective of this study was to determine the association between serum adiponectin levels and cardiometabolic risk factors in a high altitude population. We included 88 adults (31 men and 57 women), natives from Carhuamayo, town at 4146 m. Anthropometric data were recorded and blood pressure was measured. Serum levels of lipidic profile, glucose and adiponectin were determined. Mean values of serum adiponectin in women were found to be significantly higher than those in men. Estimated hipoadiponectinemia (<4 μg/mL) was 23,9% in the total sample, 45,2% in men and 12,4% in women; the serum adiponectin highest prevalence (47,6%) corresponded to the hipoadiponectinemic group. Serum adiponectin level showed significantly negative correlation with BMI and with serum triglyceride concentration, and was positively and significantly correlated with HDL-cholesterol. Was concluded that hipo...

The influence of hypobaric hypoxia on protein oxidation in lungs, heart, liver, kidneys and testicles of high altitude native guinea pigs (Huancavelica, 3660 m) in comparison to sea level (Lima, 150 m) native guinea pigs was evaluated. The concentration of protein hydroperoxides (POOH) and carbonyl groups (GC) as markers of protein oxidation, as well as total thiols (TT) concentration, powerful reducing agents that act as live antioxidants were determined. The results showed low concentration of GC in high altitude guinea pig testicles, as compared to what was found at sea level. POOH low concentration was also found in high altitude guinea pig heart with respect to that found at sea level. TT concentration was lower in high altitude heart and lungs in relation to the concentrations found at sea level. Contrary to these results, higher concentrations of POOH in high altitude guinea pig l...

The aim of this study was to evaluate the hepatoprotective effect of Aloe vera modified gel (MAVG) against the injury induced in albino mice by the oral administration of mixed overheated cooking oil (OCO) with dimethyl sulfoxide (DMSO) (1:1). OCO doses used were 2,3 and 3,1 mL/kg administered for 14 days, which produced increases of transaminases ALT, AST and triglycerides. For the specimens that received the lowest dose of OCO (2,3 mL/kg body weight), since day 14 and continuing for 18 days, were administered additionally four drops daily of MAVG equivalent to 6,1 ml/kg. When analyzing the results obtained at 14, 19, 28 and 33 days of starting treatment were observed significant progressive decrease in ALT, AST and triglycerides. However, the specimens that received the highest dose of OCO (3,1 ml/kg) plus MAVG, showed increase in the levels of ALT, AST and triglycerides over time, sug...

The objective of this study was to determine the association between serum adiponectin levels and cardiometabolic risk factors in a high altitude population. We included 88 adults (31 men and 57 women), natives from Carhuamayo, town at 4146 m. Anthropometric data were recorded and blood pressure was measured. Serum levels of lipidic profile, glucose and adiponectin were determined. Mean values of serum adiponectin in women were found to be significantly higher than those in men. Estimated hipoadiponectinemia (<4 μg/mL) was 23,9% in the total sample, 45,2% in men and 12,4% in women; the serum adiponectin highest prevalence (47,6%) corresponded to the hipoadiponectinemic group. Serum adiponectin level showed significantly negative correlation with BMI and with serum triglyceride concentration, and was positively and significantly correlated with HDL-cholesterol. Was concluded that hipo...

The influence of hypobaric hypoxia on protein oxidation in lungs, heart, liver, kidneys and testicles of high altitude native guinea pigs (Huancavelica, 3660 m) in comparison to sea level (Lima, 150 m) native guinea pigs was evaluated. The concentration of protein hydroperoxides (POOH) and carbonyl groups (GC) as markers of protein oxidation, as well as total thiols (TT) concentration, powerful reducing agents that act as live antioxidants were determined. The results showed low concentration of GC in high altitude guinea pig testicles, as compared to what was found at sea level. POOH low concentration was also found in high altitude guinea pig heart with respect to that found at sea level. TT concentration was lower in high altitude heart and lungs in relation to the concentrations found at sea level. Contrary to these results, higher concentrations of POOH in high altitude guinea pig l...

El objetivo del presente estudio fue evaluar el efecto hepatoprotector del gel de Aloe vera modificado (GAVM) frente a la injuria inducida en ratones albinos por la administración, vía oral, de aceite comestible recalentado (ACR) mezclado con dimetilsulfóxido (DMSO) (1:1). Las dosis de ACR usadas fueron 2,3 y 3,1 mL/kg, administradas por 14 días, lo cual produjo incremento de las transaminasas (ALT, AST) y triglicéridos. A los ejemplares que recibieron la menor dosis de ACR (2,3 mL/kg de peso), a partir del día 14 y por 18 días, se les suministró, adicionalmente, cuatro gotas diarias de GAVM equivalente a 6,1 mL/kg de peso. Al analizar los resultados obtenidos a los 14, 19, 28 y 33 días de iniciado el tratamiento, se observó disminución progresiva significativa de ALT, AST y triglicéridos. Sin embargo, los ejemplares que recibieron mayor dosis de ACR (3,1 mL/kg de peso) más ...

Objective: To evaluate acute oral toxicity by the method of the decoction classes of the aqueous extract of leaves and stems of Desmodium molliculum (H.B.K.) D.C., manayupa (EAM), at a maximum concentration of 2000 mg / kg body weight. Biological material: Leaves and stems of Desmodium molliculum, manayupa and Mus musculus strain Balb / c / CNPB, adult male and female mice. Methodology: The leaves and stems of Desmodium molliculum, manayupa collected in La Encañada-Cajamarca were desiccated and crushed to 1.5 mm, subjected to a hot aqueous extraction, filtered and concentrated by lyophilization. The acute oral toxicity study was performed using doses of 500, 1000 and 2000 mg / kg bw, respectively. The observations made were recorded that were aimed at the determination of: signs and symptoms of toxicity, weight, death and time of occurrence of the same on days 1, 7 and 14. Results: Unde...

Objective: To evaluate acute oral toxicity by the method of the decoction classes of the aqueous extract of leaves and stems of Desmodium molliculum (H.B.K.) D.C., manayupa (EAM), at a maximum concentration of 2000 mg / kg body weight. Biological material: Leaves and stems of Desmodium molliculum, manayupa and Mus musculus strain Balb / c / CNPB, adult male and female mice. Methodology: The leaves and stems of Desmodium molliculum, manayupa collected in La Encañada-Cajamarca were desiccated and crushed to 1.5 mm, subjected to a hot aqueous extraction, filtered and concentrated by lyophilization. The acute oral toxicity study was performed using doses of 500, 1000 and 2000 mg / kg bw, respectively. The observations made were recorded that were aimed at the determination of: signs and symptoms of toxicity, weight, death and time of occurrence of the same on days 1, 7 and 14. Results: Unde...

Objectives: To determine growth hormone (GH) serum levels in adult males at sea level (Lima, 150 msnm) and high altitude (Cajamarca: 2 750 msnm, Huancayo: 3 280 msnm, Morococha: 4 540 msnm). Design: Descriptive, observational, transversal and prospective study. Location: Lima, Cajamarca, Huancayo y Morococha, Peru. Participants: Male adult natives of different altitude settings. Interventions: With informed consent blood samples were obtained from the forearm in fasting and resting conditions from 41 young adult male subjects 20 to 35 years old [12 subjects at sea level (Lima 150 m), 10 subjects in Cajamarca (2 750 m), 12 subjects in Huancayo (3 280 m), and 7 subjects in Morococha (4 540 m)]. Main outcome measures: Serum growth hormone (GH) levels. Results: GH mean values found were at 4 540 m: 3,87 ± 0,16 ng/mL; at 3 280 m: 1,31 ± 0,09 ng/mL; at 2 750 m: 0,63 ± 0,08 ng/mL; and at 150...

Objetivos: Estudiar el efecto del mate de coca en los niveles hormonales: GH, PRL, ACTH, cortisol, FSH, LH, T, E2. Diseño: Estudio experimental. Institución: Instituto Nacional de Biología Andina, Instituto Investigaciones Clínicas y Sección Fisiología, Facultad de Medicina, UNMSM. Participantes: Varones jóvenes nativos del nivel del mar. Intervenciones: Se evaluó los parámetros metabólicos en estudio en Lima, 150 m (NM), al arribo a Huancayo, 3 280 m (ALT), después de un ejercicio submáximo y luego de un ejercicio submáximo después de tomar mate de coca. Los resultados fueron analizados con el paquete estadístico SPSS 19, mediante la pruebas no paramétricas de Friedman y la prueba de Wilcoxon. Principales medidas de resultados: GH, PRL, ACTH, cortisol, FSH, LH, T, E2 en suero. Resultados: (NM, ejercicio-ALT, ejercicio-ALT-MC): GH (mg/dL) 9,82±1,03, 11,11±2,42, 9,10±1,...

Objetivos: Estudiar el efecto del mate de coca en la función cardio-respiratoria en jóvenes sometidos a ejercicio sub- máximo, en la exposición aguda a la altura. Diseño: Experimental. Institución: Instituto Nacional de Biología Andina y Sección Fisiología, Facultad Medicina, UNMSM. Participantes: Varones del nivel del mar. Intervenciones: Se evaluó la función respiratoria y cardiovascular en Lima, a 150 m (NM) y al arribo a Huancayo, a 3 280 m (ALT), después de un ejercicio sub máximo y luego de un ejercicio sub máximo después de tomar mate de coca. Principales medidas de resultados: CVF, VEF1, %VF1, VEF25-75%, PEF; PAS, PAD, FC, Sat O2, EKG. Resultados: (NM, ejercicio-ALT, ejercicio-ALT-MC): CVF (%) 95,71±5,35, 97±5,67, 97,86±5,82. VEF1 (%) 99,86±5,35, 99,14±6,73, 102,14±6,26; %VEF1 89,48±1,65, 87,95±4,24, 89,58±2,44, VEF25-75% 116,57±8,31, 118,86±15,33, 119±...