Throughout the world, interest toward the search of natural sources of betacarotene has beenincreased due to its wide use as a colorant and antioxidant agent in pharmaceutical, cosmetic and food industries. In order to isolate Archaea halophilics betacarotene producers, salt samples from solar saltems located in Huacho were taken and cultured in sea water agar (SWA) supplemented with 20% NaCl and 0.5% yeast extract.. From the 25 microorganisms isolated, 12 which presented difierent reddish cellular pigmentation were chosen. Selected isolates were analyzed to determine their cellular growth and ability to produce betacarotene, being the isolate W2 which obtained the highest cellular pigmentation index. Besides, optimal concentration of NaCl and yeast extract to increase the production of betacarotene was determined. Isolate W2 was identified as Haloferax gibbonsii of the Archaea domain by...

Throughout the world, interest toward the search of natural sources of betacarotene has beenincreased due to its wide use as a colorant and antioxidant agent in pharmaceutical, cosmetic and food industries. In order to isolate Archaea halophilics betacarotene producers, salt samples from solar saltems located in Huacho were taken and cultured in sea water agar (SWA) supplemented with 20% NaCl and 0.5% yeast extract.. From the 25 microorganisms isolated, 12 which presented difierent reddish cellular pigmentation were chosen. Selected isolates were analyzed to determine their cellular growth and ability to produce betacarotene, being the isolate W2 which obtained the highest cellular pigmentation index. Besides, optimal concentration of NaCl and yeast extract to increase the production of betacarotene was determined. Isolate W2 was identified as Haloferax gibbonsii of the Archaea domain by...

The influence of hypobaric hypoxia on protein oxidation in lungs, heart, liver, kidneys and testicles of high altitude native guinea pigs (Huancavelica, 3660 m) in comparison to sea level (Lima, 150 m) native guinea pigs was evaluated. The concentration of protein hydroperoxides (POOH) and carbonyl groups (GC) as markers of protein oxidation, as well as total thiols (TT) concentration, powerful reducing agents that act as live antioxidants were determined. The results showed low concentration of GC in high altitude guinea pig testicles, as compared to what was found at sea level. POOH low concentration was also found in high altitude guinea pig heart with respect to that found at sea level. TT concentration was lower in high altitude heart and lungs in relation to the concentrations found at sea level. Contrary to these results, higher concentrations of POOH in high altitude guinea pig l...

The influence of hypobaric hypoxia on protein oxidation in lungs, heart, liver, kidneys and testicles of high altitude native guinea pigs (Huancavelica, 3660 m) in comparison to sea level (Lima, 150 m) native guinea pigs was evaluated. The concentration of protein hydroperoxides (POOH) and carbonyl groups (GC) as markers of protein oxidation, as well as total thiols (TT) concentration, powerful reducing agents that act as live antioxidants were determined. The results showed low concentration of GC in high altitude guinea pig testicles, as compared to what was found at sea level. POOH low concentration was also found in high altitude guinea pig heart with respect to that found at sea level. TT concentration was lower in high altitude heart and lungs in relation to the concentrations found at sea level. Contrary to these results, higher concentrations of POOH in high altitude guinea pig l...

El objetivo del presente trabajo fue determinar las concentraciones séricas de la mieloperoxidasa (MPO), una enzima considerada como indicadora de inflamación, en una población de las grandes alturas y en otra del nivel del mar y establecer la asociación de esta enzima con los factores de riesgo tendientes a desarrollar una enfermedad cardiometabólica. Se recolectó muestras de sangre de 65 personas oriundas de la ciudad de Carhuamayo (4100 m - Junín) y de 42 personas de Mala (30 m - Lima). Se midió peso, talla y presión arterial. Se determinó hemoglobina (Hb) en sangre total; glucosa, colesterol total, colesterol de lipoproteínas de alta densidad, triglicéridos y MPO se determinaron en suero. Los resultados muestran que a excepción del peso, talla y Hb no hay diferencia significativa por género entre los promedios de las demás variables de ambos grupos. Al comparar ambas p...