Throughout the world, interest toward the search of natural sources of betacarotene has beenincreased due to its wide use as a colorant and antioxidant agent in pharmaceutical, cosmetic and food industries. In order to isolate Archaea halophilics betacarotene producers, salt samples from solar saltems located in Huacho were taken and cultured in sea water agar (SWA) supplemented with 20% NaCl and 0.5% yeast extract.. From the 25 microorganisms isolated, 12 which presented difierent reddish cellular pigmentation were chosen. Selected isolates were analyzed to determine their cellular growth and ability to produce betacarotene, being the isolate W2 which obtained the highest cellular pigmentation index. Besides, optimal concentration of NaCl and yeast extract to increase the production of betacarotene was determined. Isolate W2 was identified as Haloferax gibbonsii of the Archaea domain by...

Infectious Coriza (IC) is a disease of the upper respiratory tract of commerdal birds, is caused by Haemophilus paragallinarum, prevented through vaccines and treated by several antimicrobials. With the objective of determining the sensitivity of most used-anti Infectious Coriza antimicrobials, nineteen strains of H. paragallinarum isolated from beeders layer hens and broiler chickens with typical symptoms of IC, from different avian regions of Peru were used. The minimal inhibitory concentrations to trimethoprim, sulfamethoxazole, amoxicillin, gentamicin and enrofloxacin were determined through the microdilution method reconunended by the National Committee for Clinical Laboratory Standards. All strains of H paragallinarum were resistant to sulfamethoxazole; intermediate sensitivity and / or resistant to trimethoprimand and trimethoprim-sulfamethoxazole, gentamicin and amoxicillin, but ...

Throughout the world, interest toward the search of natural sources of betacarotene has beenincreased due to its wide use as a colorant and antioxidant agent in pharmaceutical, cosmetic and food industries. In order to isolate Archaea halophilics betacarotene producers, salt samples from solar saltems located in Huacho were taken and cultured in sea water agar (SWA) supplemented with 20% NaCl and 0.5% yeast extract.. From the 25 microorganisms isolated, 12 which presented difierent reddish cellular pigmentation were chosen. Selected isolates were analyzed to determine their cellular growth and ability to produce betacarotene, being the isolate W2 which obtained the highest cellular pigmentation index. Besides, optimal concentration of NaCl and yeast extract to increase the production of betacarotene was determined. Isolate W2 was identified as Haloferax gibbonsii of the Archaea domain by...

Infectious Coriza (IC) is a disease of the upper respiratory tract of commerdal birds, is caused by Haemophilus paragallinarum, prevented through vaccines and treated by several antimicrobials. With the objective of determining the sensitivity of most used-anti Infectious Coriza antimicrobials, nineteen strains of H. paragallinarum isolated from beeders layer hens and broiler chickens with typical symptoms of IC, from different avian regions of Peru were used. The minimal inhibitory concentrations to trimethoprim, sulfamethoxazole, amoxicillin, gentamicin and enrofloxacin were determined through the microdilution method reconunended by the National Committee for Clinical Laboratory Standards. All strains of H paragallinarum were resistant to sulfamethoxazole; intermediate sensitivity and / or resistant to trimethoprimand and trimethoprim-sulfamethoxazole, gentamicin and amoxicillin, but ...

Halophilic bacteria produce a great variety of substances and enzymes with interesting biotechnological applications. In order to isolate moderately halophilic bacteria from saline environments in Atacocha (Ayacucho}, salt samples were collected and inoculated in saline medium (5% total salts) with 0.5% yeast extract. After one week incubation they were streaked onto agar plates prepared with the same saline medium as before but solidified with 2% agar. Ten, out of 40 isolates, were selected based on their differential cultural and morphological features. Further characterization of the selected isolated included tolerance to NaCl, temperature and pH ranges for growth and some biochemical and nutritional tests. The strains tolerated 0.1 to 4.0 M NaCl and had their optima between 1.5 and 2.0 M, the temperatures and pH values enabling growth were 4 to 37 ºC and 5 to 9, respectively. The m...

Las bacterias halófilas producen una gran variedad de metabolitos y enzimas con interesantes aplicaciones bíotecnológicas. Con la finalidad de aislar bacterias halófilas moderadas de las minas salinas de Atacocha - Ayacucho, se recolectaron muestras de sal y se preenriquecieron en medio agua de sales al 5% suplementado con extracto de levadura al 0.5% durante una semana y luego se sembraron en medio sólido. De 40 aislados se seleccionaron 10 por presentar características morfológicas y culturales muy diferenciadas. A los aislados seleccionados se les determinó el rango de tolerancia al NaCl, temperatura, pH y sus características bioquímicas y nutricionales. Se encontró que los aislados toleraban cantidades de sal diferentes entre 0.1 y 4.0 M de NaCl, pero sus óptimos de NaCl se situaron entre 1.5 y 2.0 M, los rangos de temperaturas estuvieron entre 4 y 37 ºC y los de pH entr...

The CYP2D6 gene metabolizes approximately 25 % of drugs of clinical use and exhibits a marked genetic variation, thus according to the allelic variants carried by each individual three types of metabolism can be observed: poor (PM), extensive (EM) or ultrarapid (UM). There is also interethnic variation in the frequencies of PM, EM and UM individuals. Knowledge of the individual CYP2D6 metabolic status may be clinically and economically important and could provide the basis for a rational approach to drug prescription.

El gen CYP2D6 metaboliza aproximadamente el 25% de fármacos de uso clínico y exhibe una marcada variación genética, por lo que como resultado de las variantes alélicas que porte cada individuo se pueden presentar tres tipos de metabolismo: lento (PM), rápido (EM) o ultrarrápido (UM). Dependiendo del origen étnico de la población se han observado diferencias en las frecuencias de individuos PM, EM y UM. El conocimiento individual del fenotipo CYP2D6 es clínica y económicamente importante, ya que disminuye el riesgo a reacciones adversas y proporciona las bases para una mejor prescripción farmacológica.