Infectious Coriza (IC) is a disease of the upper respiratory tract of commerdal birds, is caused by Haemophilus paragallinarum, prevented through vaccines and treated by several antimicrobials. With the objective of determining the sensitivity of most used-anti Infectious Coriza antimicrobials, nineteen strains of H. paragallinarum isolated from beeders layer hens and broiler chickens with typical symptoms of IC, from different avian regions of Peru were used. The minimal inhibitory concentrations to trimethoprim, sulfamethoxazole, amoxicillin, gentamicin and enrofloxacin were determined through the microdilution method reconunended by the National Committee for Clinical Laboratory Standards. All strains of H paragallinarum were resistant to sulfamethoxazole; intermediate sensitivity and / or resistant to trimethoprimand and trimethoprim-sulfamethoxazole, gentamicin and amoxicillin, but ...

Infectious Coriza (IC) is a disease of the upper respiratory tract of commerdal birds, is caused by Haemophilus paragallinarum, prevented through vaccines and treated by several antimicrobials. With the objective of determining the sensitivity of most used-anti Infectious Coriza antimicrobials, nineteen strains of H. paragallinarum isolated from beeders layer hens and broiler chickens with typical symptoms of IC, from different avian regions of Peru were used. The minimal inhibitory concentrations to trimethoprim, sulfamethoxazole, amoxicillin, gentamicin and enrofloxacin were determined through the microdilution method reconunended by the National Committee for Clinical Laboratory Standards. All strains of H paragallinarum were resistant to sulfamethoxazole; intermediate sensitivity and / or resistant to trimethoprimand and trimethoprim-sulfamethoxazole, gentamicin and amoxicillin, but ...

The study aimed to quantify the presence of micotoxins ochratoxine A and toxin T-2 in two common feedstuffs used in poultry diets (yellow corn and soybean meal) from different origins. Samples received for analysis from 2007 to 2011 were included in the study: 139 samples of corn and 64 of soybean meal were analyzed for ochratoxine A and 193 samples of corn and 144 of soybean were analized for toxin T-2. Results showed 66.2 and 67.4% positive samples of maize to ochratoxine A and toxin T-2 respectively, and 71.9 y 88.9% positive samples of soybean for ochratoxine A and toxin T-2 respectively. None of the samples surpassed the accepted limits recommended by the European Commission (2006/576/EC).

El estudio tuvo como objetivo cuantificar la presencia de las micotoxinas ocratoxina A y toxina T-2 en dos ingredientes alimenticios comunes en las dietas avícolas de procedencia diversa. Se analizaron 139 muestras de maíz y 64 de torta de soya para el contenido de ocratoxina A y 193 muestras de maíz y 144 de torta de soya para el contenido de toxina T-2, las mismas que fueron recibidas para análisis entre 2007 y 2011. Los resultados indicaron 66.2 y 67.4% de muestras positivas de maíz para ocratoxina A y toxina T-2, respectivamente. Asimismo, 71.9 y 88.9% muestras positivas de torta de soya para ocratoxina A y toxina T-2, respectivamente. Ninguna muestra llegó a sobrepasar los límites de ocratoxina A y T-2 permitidos, según las recomendaciones de la Comisión Europea (2006/576/EC).

The aim of the study was to identify biovars of Pasteurella multocida and Gallibacterium anatis in poultry with respiratory signs. These bacteria were isolated from samples of secretions and organs of affected broilers, layers and ducks from various poultry farms in the coast and the tropics of Peru. Out of 25 isolates obtained, 13 strains of P. multocida and 12 of G. anatis were identified based on culture characteristics, morphology and biochemical tests (oxidase, catalase, indole, urease). These strains were subjected to micro-well fermentation tests with 10 carbohydrates and one amino acid for typification. Results showed that eight strains of P. multocida corresponded to biovar 1 and the others to biovars 3, 4, 6, 10 and 11, while 11 strains of G. anatis corresponded to biovar haemolytica and one strain to biovar anatis.

The aim of the study was to identify biovars of Pasteurella multocida and Gallibacterium anatis in poultry with respiratory signs. These bacteria were isolated from samples of secretions and organs of affected broilers, layers and ducks from various poultry farms in the coast and the tropics of Peru. Out of 25 isolates obtained, 13 strains of P. multocida and 12 of G. anatis were identified based on culture characteristics, morphology and biochemical tests (oxidase, catalase, indole, urease). These strains were subjected to micro-well fermentation tests with 10 carbohydrates and one amino acid for typification. Results showed that eight strains of P. multocida corresponded to biovar 1 and the others to biovars 3, 4, 6, 10 and 11, while 11 strains of G. anatis corresponded to biovar haemolytica and one strain to biovar anatis.

In order to standardize a rapid method for detecting C. jejuni in chickens, it was taken cloacal swabs from 50 chickens of seven-week lifetime belonging to several markets of Lima's city. DNA was extracted by using organic solvents and the presence of C. jejuni was detected by means of Nested-PCR using specific primers for 16S ribosomal and hipO genes. In parallel with this rapid DNA-based approach, it was isolated C. jejuni by conventional filtration bacteriological methods and selective culture mediums. The rapid and conventional methods used in this study, detected C jejuni in 48/50 (96%) and 29/50 (58%) respectively. These findings indicate than the rapid method has a higher sensitivity lhan the conventional one.

In order to standardize a rapid method for detecting C. jejuni in chickens, it was taken cloacal swabs from 50 chickens of seven-week lifetime belonging to several markets of Lima's city. DNA was extracted by using organic solvents and the presence of C. jejuni was detected by means of Nested-PCR using specific primers for 16S ribosomal and hipO genes. In parallel with this rapid DNA-based approach, it was isolated C. jejuni by conventional filtration bacteriological methods and selective culture mediums. The rapid and conventional methods used in this study, detected C jejuni in 48/50 (96%) and 29/50 (58%) respectively. These findings indicate than the rapid method has a higher sensitivity lhan the conventional one.

El objetivo del presente estudio fue determinar la presencia de factores de virulencia y patrones de resistencia antibiótica en 46 cepas de Escherichia coli aisladas en aves de corral (Pollos de carne - Broiler). Las 46 cepas de Escherichia coli fueron resistentes a ciprofloxacina, enrofloxacina, colistina, amoxicilina, florfenicol, doxiciclina, oxitetraciclina, fosfomicina y sulfatrimetropin. Análisis molecular de REP-PCR y BOXA1R confirmó la presencia de 46 cepas genéticamente diferentes obtenidas a partir de los aislados clínicos. Toxinotipificación mediante PCR para los genes de Toxina Shiga 1 (stx1), Toxina Shiga 2 (stx2) encontró solo la presencia de genes productores de Toxina Shiga 2 en 6 cepas de Escherichia coli (6/46; 13,04 %). El presente estudio constituye uno de los primeros reportes de Escherichia coli productora de shigatoxina 2 en sistemas de producción de pollos...

The purpose of this study was to determine the genetic variability of strains of Gallibacterium anatis isolated in commercial poultry with respiratory symptoms from Arequipa (2), Ica (3), La Libertad (27), Lima (62), Madre de Dios (1) and Ucayali (1), collected from 2007 to 2011. Strains were typified by carbohydrate fermentation tests and sensitivity to nine antimicrobials. The genetic variability of these strains was determined by ERIC-PCR, after identification by PCR using specific primers. The analysis of carbohydrate fermentation profiles corresponded to 10 biovars, all of which were found in Lima and 45.8% of the strains belonged to biovar 4. Concerning antimicrobial susceptibility, 40.8% of strains were resistant to all antimicrobials studied, while 94.8 and 95.8% were resistant to enrofloxacin and ciprofloxacin. By ERIC-PCR 24 DNA profiles associated with biovars were obtained, i...

El propósito del estudio fue determinar la variabilidad genética de 96 cepas de Gallibacterium anatis aisladas de aves comerciales con sintomatología respiratoria, provenientes de Arequipa (2), Ica (3), La Libertad (27), Lima (62), Madre de Dios (1) y Ucayali (1), y recolectadas desde el 2007 al 2011. Las cepas se tipificaron mediante pruebas de fermentación de carbohidratos y sensibilidad a nueve antimicrobianos. La variabilidad genética de las cepas de G. anatis se determinó mediante ERIC-PCR, previa identificación por PCR usando cebadores específicos. Del análisis de fermentación de carbohidratos se obtuvieron perfiles que correspondieron a 10 biovares, todos los cuales se encontraron en Lima y el 45.8% de las cepas pertenecieron al biovar 4. Con respecto a la sensibilidad antimicrobiana, el 40.8% de las cepas fueron resistentes a todos los antimicrobianos estudiados, en tan...