Phospholipases A2 (PLA2) are enzymes that belong to a great protein family, at the moment classified in 12 groups; that they share similar enzymatic function and structure. In agreement with the biochemical characteristics and the cellular origin, phospholipases are classified like: citosolic (cPCLA2), secretory (sPLA2) and intracellular (iPLA2). The relation structure-activity, of this protein group is a challenge for biochemists, molecular biologists, toxicologists, pharmacologists and physiologists. PLA2 has been identified in tissues of mammals, mainly in diverse snake venoms and some bacteria and plants. Numerous physiological and physiopathological functions without toxicity have been attributed to PLA2 of mammals. In oppsosite, PLA2 of venoms are toxic and induce pharmacological effects that are half-full probably by membrane receivers, diverse isoenzimas of PLA2 of venoms are inv...

In order to standardize a rapid method for detecting C. jejuni in chickens, it was taken cloacal swabs from 50 chickens of seven-week lifetime belonging to several markets of Lima's city. DNA was extracted by using organic solvents and the presence of C. jejuni was detected by means of Nested-PCR using specific primers for 16S ribosomal and hipO genes. In parallel with this rapid DNA-based approach, it was isolated C. jejuni by conventional filtration bacteriological methods and selective culture mediums. The rapid and conventional methods used in this study, detected C jejuni in 48/50 (96%) and 29/50 (58%) respectively. These findings indicate than the rapid method has a higher sensitivity lhan the conventional one.

Phospholipases A2 (PLA2) are enzymes that belong to a great protein family, at the moment classified in 12 groups; that they share similar enzymatic function and structure. In agreement with the biochemical characteristics and the cellular origin, phospholipases are classified like: citosolic (cPCLA2), secretory (sPLA2) and intracellular (iPLA2). The relation structure-activity, of this protein group is a challenge for biochemists, molecular biologists, toxicologists, pharmacologists and physiologists. PLA2 has been identified in tissues of mammals, mainly in diverse snake venoms and some bacteria and plants. Numerous physiological and physiopathological functions without toxicity have been attributed to PLA2 of mammals. In oppsosite, PLA2 of venoms are toxic and induce pharmacological effects that are half-full probably by membrane receivers, diverse isoenzimas of PLA2 of venoms are inv...

In order to standardize a rapid method for detecting C. jejuni in chickens, it was taken cloacal swabs from 50 chickens of seven-week lifetime belonging to several markets of Lima's city. DNA was extracted by using organic solvents and the presence of C. jejuni was detected by means of Nested-PCR using specific primers for 16S ribosomal and hipO genes. In parallel with this rapid DNA-based approach, it was isolated C. jejuni by conventional filtration bacteriological methods and selective culture mediums. The rapid and conventional methods used in this study, detected C jejuni in 48/50 (96%) and 29/50 (58%) respectively. These findings indicate than the rapid method has a higher sensitivity lhan the conventional one.