A rapid detection of Campylobacter jejuni in chickens by nested polymerase chain reaction

• Autores: Jiménez, Karim L., Zavaleta, Amparo I., lzaguirre, Victor, Koga, Ysabel, Soto, Javier
• Formato: artículo
• Fecha de Publicación: 2007
• Institución: Universidad Nacional Mayor de San Marcos
• Repositorio: Revista UNMSM - Ciencia e Investigación
• Lenguaje: español
• OAI Identifier: oai:ojs.csi.unmsm:article/5634
• Enlace del recurso: https://revistasinvestigacion.unmsm.edu.pe/index.php/farma/article/view/5634
• Nivel de acceso: acceso abierto
• Materia: C. jejuni; Nested-PCR; 16S ribosomal genes; hipO gen; chickens; C. jejun; PCR anidada; genes ribosómicos 16S; gen hipo; pollos

In order to standardize a rapid method for detecting C. jejuni in chickens, it was taken cloacal swabs from 50 chickens of seven-week lifetime belonging to several markets of Lima's city. DNA was extracted by using organic solvents and the presence of C. jejuni was detected by means of Nested-PCR using specific primers for 16S ribosomal and hipO genes. In parallel with this rapid DNA-based approach, it was isolated C. jejuni by conventional filtration bacteriological methods and selective culture mediums. The rapid and conventional methods used in this study, detected C jejuni in 48/50 (96%) and 29/50 (58%) respectively. These findings indicate than the rapid method has a higher sensitivity lhan the conventional one.