Este siglo XXI, caracterizado como el siglo de la racionalidad científica y tecnológica, porque el saber cambia el mundo y la sociedad del conocimiento, la ciencia y la tecnología, que conquistan el bienestar y la salud para el hombre. El objetivo fundamental del profesional químico farmacéutico es salvaguardar el derecho de la sociedad a que se le otorgue calidad, seguridad, eficacia y dispensación responsable de los medicamentos, con estricta observancia de las normativas de la Organización Mundial de la Salud, que el profesional farmacéutico asume, en su condición de experto en el medicamento, el alimento y el tóxico.

Este siglo XXI, caracterizado como el siglo de la racionalidad científica y tecnológica, porque el saber cambia el mundo y la sociedad del conocimiento, la ciencia y la tecnología, que conquistan el bienestar y la salud para el hombre. El objetivo fundamental del profesional químico farmacéutico es salvaguardar el derecho de la sociedad a que se le otorgue calidad, seguridad, eficacia y dispensación responsable de los medicamentos, con estricta observancia de las normativas de la Organización Mundial de la Salud, que el profesional farmacéutico asume, en su condición de experto en el medicamento, el alimento y el tóxico.

In the present study were evaluated the bioactive compounds and antioxidant activity and satisfaction degree of a drink made with purple corn and stevia. Were used HPLC, ABTS, ORAC, DPPH methods and was done comparison according to the hedonic scale. Results showed close phenolic compounds to 2 mg/mL, high in anthocyanins (50,84 mg/100 mL) and antioxidant activity with ABTS (TEAC 17,96 pmol/mL), ORAC (31,01 μmol TEAC/mL) and DPPH (GAE 0,706 mg/mL). The useful life assessment revealed a synergistic effect between the purple corn anthocyanins and the active components of stevia. The satisfaction degree revealed that the formulation was described between "really like " (19%) and "like slightly " (33%) according to the hedonic scale about attributes of color, smell, taste and overall acceptability.

En la presente investigación se evaluaron los compuestos bioactivos, y se determinaron la actividad antioxidante y el grado de satisfacción de una bebida de maíz morado y estevia, mediante los métodos por HPLC, ABTS, ORAC, DPPH y por comparación según la escala hedónica. Los resultados mostraron compuestos fenólicos cercanos a 2 mg/mL, altos contenidos de antocianinas (50,84 mg/100 mL) y actividad antioxidante según ABTS (17,96 μmol TEAC/mL), ORAC (31,01 μmol TEAC/mL) y DPPH (0,706 GAE mg/mL). La evaluación de vida útil reveló un efecto sinérgico entre las antocianinas del maíz morado y los componentes activos de la estevia. El grado de satisfacción reveló que el formulado se califica entre “me gusta mucho” (19%) y “me gusta ligeramente” (33%) según escala hedónica sobre los atributos de color, olor, sabor y aceptabilidad en general.

The aim of this study was evaluate presence of flavonoids and saponins in alcoholic extracts of leaves of Desmodium molliculum HBK " manayupa " These metabolites was valuated with the aim of contributing to a better understanding of the chemical composition of this plant. The species was collected in the district of Baños del Inca City of Cajamarca, department of Cajamarca to 2776 meters. The phytochemical march revealed the presence of tannins, flavonoids, alkaloids and saponins in the alcoholic extract. Chromatographic analysis of the alcoholic extract using isoflavones genistein and daizein and vitexin flavonoid as reference standard revealed the probable presence of these isoflavonoids and the flavonoid in the alcohólic extract. UV spectroscopic analysis allowed us to confirm one of the isoflavone as genistein and 5-O-methylgenistein in addition chromatographic analysis using metha...

The Piper aduncum, also known as “Matico” in Peru, is a plant used as a healing, anti-diarrheal, anti-inflammatory, anti-cancer and other traditional uses plus1; These effects have been demonstrated as the antimicrobial2 and cytotoxic effect, the latter using bioguided fractionation, observing the effect on leaf extracts with dichloromethane on KB cells (nasopharyngeal carcinoma)3 and the essential oil on lung carcinoma, colon adenocarcinomas, adenocarcinoma of breast, amelanotic melanoma, cervical carcinoma, prostate carcinomas, chronic myeloid leukemia and normal mouse fibroblasts; in addition to oral toxicity in mice, finding null and toxic antitumor effect4. In the present study, the in vitro antiproliferative activity of the methanolic extract of Piper aduncum leaves on the AGS cell line (gastric adenocarcinoma) acquired from the ECACC (European Authenticated Cell Culture Collec...

The aim of this study was evaluate presence of flavonoids and saponins in alcoholic extracts of leaves of Desmodium molliculum HBK " manayupa " These metabolites was valuated with the aim of contributing to a better understanding of the chemical composition of this plant. The species was collected in the district of Baños del Inca City of Cajamarca, department of Cajamarca to 2776 meters. The phytochemical march revealed the presence of tannins, flavonoids, alkaloids and saponins in the alcoholic extract. Chromatographic analysis of the alcoholic extract using isoflavones genistein and daizein and vitexin flavonoid as reference standard revealed the probable presence of these isoflavonoids and the flavonoid in the alcohólic extract. UV spectroscopic analysis allowed us to confirm one of the isoflavone as genistein and 5-O-methylgenistein in addition chromatographic analysis using metha...

The Piper aduncum, also known as “Matico” in Peru, is a plant used as a healing, anti-diarrheal, anti-inflammatory, anti-cancer and other traditional uses plus1; These effects have been demonstrated as the antimicrobial2 and cytotoxic effect, the latter using bioguided fractionation, observing the effect on leaf extracts with dichloromethane on KB cells (nasopharyngeal carcinoma)3 and the essential oil on lung carcinoma, colon adenocarcinomas, adenocarcinoma of breast, amelanotic melanoma, cervical carcinoma, prostate carcinomas, chronic myeloid leukemia and normal mouse fibroblasts; in addition to oral toxicity in mice, finding null and toxic antitumor effect4. In the present study, the in vitro antiproliferative activity of the methanolic extract of Piper aduncum leaves on the AGS cell line (gastric adenocarcinoma) acquired from the ECACC (European Authenticated Cell Culture Collec...

The purpose of the present investigation has been to observe the effect of the lipidic fractions of Leoidium meyenii Walpers, «maca» on the reproductive organs of female mice, following the method adapted by S. Garg et al (1998) The yellow maca from central andean rcgion (Puna, Junín) was used. Initially we obtained the hexanic and ethanolic éxtracts by means of saponification of the first one, fatty acids and total sterols were separated. Lipidic fractions, which were oraily administered to four groups of female mice in dosis of 200 and 100 mg/kg respectively for a period of 16 days, maintaining the control group. For the histological observation the animals were sacrificed and their avaries were fixed previous to its inclusion in paraffin.The results demostrated a significant stimulative effect on the folliclegenesis with the total sterols fraction on the ovaries of the evaluated a...

In the present investigation we have studied some fungae which produce Mycotoxins. The samples were obtained from markets in the city of Lima during the summer, autumn and spring of 1999. The method used was the expositión of the samples in agar-potatoe-dextrose and cloranfenical media. We were able to isolate eleven varieties of fungae which produce mycotoxins such as Penicillium, Aspergillus Fusarium, Altemaria, Cephalosporium, Cladosporium, Paecylomices, Phytomices, Stachybotris, Trichoderma, Trichotecium (Fig. 1,2, 3). The mast frequent ane were the ones of Penicillium gender, which were observed in the following matkets: Huamantanga, Puente de Piedra, Comas, La Victoria y San Luis (Mercado de fruta) In these we got 36 rotten apples in each one af them. The mycotoxill Patulin was identified qualitatively by thin layer chromatography. Besides, we analyzed six samples of apple juice i...

Objectives: To determine the effect of Jatropha macrantha Müll. Arg 'huanarpo macho' methanol extract in rats with induced erectiledysfunction. Design: Experimental. Setting: Pharmacology Laboratory, Faculty of Medicine, Universidad Nacional Mayor de San Marcos,Lima, Peru; Pharmacy Laboratory, Universidad Nacional de San Cristobal de Huamanga, Ayacucho, Peru. Biological material:Plant collected from Ayacucho, wistar male rats 300+/-50 g. Interventions: Plant collection was done in Vilcas Huaman Province - Ayacucho.Sexual behavior, nitric oxide concentration and vasorelaxant effect were determined in isolated rats penis corpus cavernosumdistributed in the following groups: group 1 water 10 mL/kg, group 2 sildenafil 5 mg/kg, groups 3, 4 and 5 respectively methanol extract100, 200 and 300 mg/kg. Organs were isolated in groups receiving 50, 100 and 300 ug/mL of extract, L-arginine 300 uM, 3...

The purpose of the present investigation has been to observe the effect of the lipidic fractions of Leoidium meyenii Walpers, «maca» on the reproductive organs of female mice, following the method adapted by S. Garg et al (1998) The yellow maca from central andean rcgion (Puna, Junín) was used. Initially we obtained the hexanic and ethanolic éxtracts by means of saponification of the first one, fatty acids and total sterols were separated. Lipidic fractions, which were oraily administered to four groups of female mice in dosis of 200 and 100 mg/kg respectively for a period of 16 days, maintaining the control group. For the histological observation the animals were sacrificed and their avaries were fixed previous to its inclusion in paraffin.The results demostrated a significant stimulative effect on the folliclegenesis with the total sterols fraction on the ovaries of the evaluated a...

In the present investigation we have studied some fungae which produce Mycotoxins. The samples were obtained from markets in the city of Lima during the summer, autumn and spring of 1999. The method used was the expositión of the samples in agar-potatoe-dextrose and cloranfenical media. We were able to isolate eleven varieties of fungae which produce mycotoxins such as Penicillium, Aspergillus Fusarium, Altemaria, Cephalosporium, Cladosporium, Paecylomices, Phytomices, Stachybotris, Trichoderma, Trichotecium (Fig. 1,2, 3). The mast frequent ane were the ones of Penicillium gender, which were observed in the following matkets: Huamantanga, Puente de Piedra, Comas, La Victoria y San Luis (Mercado de fruta) In these we got 36 rotten apples in each one af them. The mycotoxill Patulin was identified qualitatively by thin layer chromatography. Besides, we analyzed six samples of apple juice i...

In the present study were evaluated the antioxidant, anti-inflammatory and immunomodulatory activity of chloroform extract from the leaves of Chuquiraga lessing "huamanpinta" and was performed the identification and structure elucidation of flavonoid secondary metabolites, antioxidant activity was evaluated by the method of difenilpycrilhidrazyl (DPPH) and was measured the activity of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT) and malondialdehyde (MDA). To analize) antiinflammatory activity was used subplantar edema method according Winter; and to immunomodulatory activity the method of speed of clearing of ink. Flavonoids, triterpenes, steroids, alkaloids, tannins, phenolic compounds, sesquiterpene lactones were identified. With UV spectroscopic analysis the following flavonoids were elucidated: 5,6,7-trihidroxi-4’-metoxiflavona; 3’,5,6,7-tetrahidroxi-4�...

Introduction: Bidens pilosa species belonging to the Asteraceae family, known in Peru as love dry bur, is credited with anti-inflammatory, diuretic, hepatoprotective effects. Objectives: To determine the protective effect of phenolic compounds and flavonoids extracted from Bidens pilosa L whole plant on breast cancer induced in rats by 7,12-dimethylbenz (A) anthracene (DMBA). Design: Experimental. Setting: Laboratory of Pharmacology, Faculty of Medicine, Universidad Nacional Mayor de San Marcos, Lima, Peru. Biological material: Bidens pilosa L whole plant collected in Santiago de Cao, Ascope, La Libertad, and female rats. Interventions: Phenolic compounds and flavonoids were obtained by rapid column chromatography with solvents of increasing polarity; by thin layer chromatography on a preparative scale four phenolic compounds were isolated and elucidated by UV-Visible spectroscopy with s...

In the present study were evaluated the antioxidant, anti-inflammatory and immunomodulatory activity of chloroform extract from the leaves of Chuquiraga lessing "huamanpinta" and was performed the identification and structure elucidation of flavonoid secondary metabolites, antioxidant activity was evaluated by the method of difenilpycrilhidrazyl (DPPH) and was measured the activity of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT) and malondialdehyde (MDA). To analize) antiinflammatory activity was used subplantar edema method according Winter; and to immunomodulatory activity the method of speed of clearing of ink. Flavonoids, triterpenes, steroids, alkaloids, tannins, phenolic compounds, sesquiterpene lactones were identified. With UV spectroscopic analysis the following flavonoids were elucidated: 5,6,7-trihidroxi-4’-metoxiflavona; 3’,5,6,7-tetrahidroxi-4�...

Were studied the antioxidant effect and toxicity of the hot springs of San Antonio de Putina-Puno. Were used methods of lipid antiperoxidation, malondialdehyde (expressed as TBARS), in vivo oral acute toxicity at fixed dose according to 423 OECD and the physical chemistry composition by inductively coupled plasma source mass spectrometry (ICP-MS). The antioxidant effect presented significant difference between control group (0,0527 ŋm) and the study group (0,0390 ŋm) and malondialdehyde (p<0,05) and in vivo oral acute toxicity at fixed-dose treatment. Not evident signs of intoxication, moreover, in the composition of these hot springs were found: chloride 71,9%, sodium 80,3 % and sulphate 22,7% meq. Was concluded that the thermal mineral hot springs, are not toxic, have antioxidant effect and are chlorinated, sulphate and sodium type.

Se estudiaron el efecto antioxidante y la toxicidad de las aguas termales de San Antonio de Putina-Puno, por los métodos de antiperoxidación lipídica, malondialdehido (expresado como TBARS), la toxicidad aguda oral in vivo a dosis fijas según la OECD 423 y la composición fisicoquímica por espectrometría de pesos con fuente de plasma de acoplamiento inductivo (ICP-MS). El efecto antioxidante presentó diferencia significativa entre el grupo control (0,0527 ŋm) y el grupo estudio (0,0390 ŋm) malondialdehido (p<0,05) y en la toxicidad aguda oral in vivo a dosis fijas, después del tratamiento, no se evidenciaron signos de intoxicación, además en la composición de dichas aguas termales se encontraron cloruro 71,9%, sodio 80,3% y sulfato 22,7% meq. Se concluye que las aguas termales mineromedicinales, son atóxicas, tienen efecto antioxidante y son de tipo clorurada, sulfatada ...

Objective: To evaluate acute oral toxicity by the method of the decoction classes of the aqueous extract of leaves and stems of Desmodium molliculum (H.B.K.) D.C., manayupa (EAM), at a maximum concentration of 2000 mg / kg body weight. Biological material: Leaves and stems of Desmodium molliculum, manayupa and Mus musculus strain Balb / c / CNPB, adult male and female mice. Methodology: The leaves and stems of Desmodium molliculum, manayupa collected in La Encañada-Cajamarca were desiccated and crushed to 1.5 mm, subjected to a hot aqueous extraction, filtered and concentrated by lyophilization. The acute oral toxicity study was performed using doses of 500, 1000 and 2000 mg / kg bw, respectively. The observations made were recorded that were aimed at the determination of: signs and symptoms of toxicity, weight, death and time of occurrence of the same on days 1, 7 and 14. Results: Unde...

Objetives: To determine the gastroprotector effect of Copaifera officinalis oil using indomethacin and pyloric ligature in rats. Design: Preclinical study. Setting: Faculties of Medicine, Pharmacy and Biochemistry, National University of San Marcos, Lima, Peru. Biological material: Rats and copaiba oil. Interventions: Copaiba oil was collected in Ucayali, Pucallpa. Cytoprotection was tested with indomethacin considering a normal control group, and indomethacin, copaiba and omeprazole groups. Using visual analogue scale mucosa gastric injuries were referred as those compatible with local necrosis (unviable tissue), hyperemia, flushing, and hemorrhage, and ulcers according to the modified Macallister’s scale. The anti-secretion trial used the pyloric ligature model. Twenty-four albino rats were randomized in three groups: control, copaiba oil 40 mg/kg and omeprazole 10 mg/kg, respectivel...