In the present study were evaluated the antioxidant, anti-inflammatory and immunomodulatory activity of chloroform extract from the leaves of Chuquiraga lessing "huamanpinta" and was performed the identification and structure elucidation of flavonoid secondary metabolites, antioxidant activity was evaluated by the method of difenilpycrilhidrazyl (DPPH) and was measured the activity of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT) and malondialdehyde (MDA). To analize) antiinflammatory activity was used subplantar edema method according Winter; and to immunomodulatory activity the method of speed of clearing of ink. Flavonoids, triterpenes, steroids, alkaloids, tannins, phenolic compounds, sesquiterpene lactones were identified. With UV spectroscopic analysis the following flavonoids were elucidated: 5,6,7-trihidroxi-4’-metoxiflavona; 3’,5,6,7-tetrahidroxi-4�...

In the present study were evaluated the antioxidant, anti-inflammatory and immunomodulatory activity of chloroform extract from the leaves of Chuquiraga lessing "huamanpinta" and was performed the identification and structure elucidation of flavonoid secondary metabolites, antioxidant activity was evaluated by the method of difenilpycrilhidrazyl (DPPH) and was measured the activity of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT) and malondialdehyde (MDA). To analize) antiinflammatory activity was used subplantar edema method according Winter; and to immunomodulatory activity the method of speed of clearing of ink. Flavonoids, triterpenes, steroids, alkaloids, tannins, phenolic compounds, sesquiterpene lactones were identified. With UV spectroscopic analysis the following flavonoids were elucidated: 5,6,7-trihidroxi-4’-metoxiflavona; 3’,5,6,7-tetrahidroxi-4�...

Stevia rebaudiana Bertoni is an specie with sweetening properties, and a product used as alternative to sugar and artificial sweeteners. The objective of the research was to evaluate the hypoglycemic effect of a tablet made with the atomized extract of leaves of Stevia rebaudiana Bertoni. The hypoglycemic effect was determined using the glucose tolerance test, for which it was used 05 male rats, holtzman strain, average weight 210 ± 10g, divided in 5 groups with 6 specimens each, applying to each group a different treatment. The change in glucose levels versus time were evaluated, the area under the curve (AUC) of the different treatments was calculated. The tablet produced from the atomized stevia extract showed an AUC of 210,68 ± 57, similar to insulin with an AUC of 247,43 ± 24,2. From multiple comparisons of mean AUC, using the Duncan test at 95% confidence level, it was determine...

La Stevia rebaudiana Bertoni es una especie con propiedades edulcorantes, y utilizada como un producto alternativo al azúcar y los endulzantes artificiales. El objetivo de la investigación fue evaluar la acción hipoglicemiante de una tableta elaborada con el extracto atomizado de hojas de Stevia rebaudiana Bertoni. El efecto hipoglicemiante se determinó utilizando el test de tolerancia oral a la glucosa, para el cual se ratas macho, cepa holtzman, con peso promedio de 210 ± 10g, divididas en 5 grupos de a 6 individuos cada uno, aplicándose a cada grupo un tratamiento diferente. Se evaluó la variación de los niveles de glucosa en función del tiempo, se calculó el área bajo la curva (AUC) de los diferentes tratamientos. La tableta elaborada a base del extracto atomizado de Stevia presentó un AUC de 210,68 ± 57, similar al de insulina que tiene un AUC de 247,43 ± 24,2. De las ...

The presence of ochratoxin A (OTA), a mycotoxin with carcinogenic properties, was analyzed in the beans of Theobroma cacao L. "trinitario cacao" from the collection center of Pisana-Tingo Maria, of the San Martin Region, Peru. The samples were collected in four stages of production: harvesting, fermentation, drying and storage. The OTA was extracted using 60 % acetonitrile and purified by immunoaffinity columns and subsequent quantification by high performance liquid chromatography (HPLC). The recovery percentage of the method used was evaluated by using samples fortified with OTA, showing a recovery percentage greater than 95 %. The beans evaluated in the different stages of production did not show the presence of ochratoxin A and in the mycotoxigenic analysis the presence of fungi of the genus Fusarium, Penicillium and yeasts was evidenced.

Objective. To assess the effect of Banisteriopsis caapi and Psychotria viridis ‘binomio ayahuasca’ ingestion in rat hippocampus. Design. Experimental, descriptive, analytical, and cross-sectional study. Institution. Research Institute of Pharmaceutical Sciences and Natural Resources, Faculty of Pharmacy and Biochemistry, Universidad Nacional Mayor de San Marcos, Lima, Peru. Biological material. Rats. Interventions. Orogastric administration of ‘binomio ayahuasca’ in adult Rattus norvegicus albinus Holtzman strain. Seven groups of five animals with an average weight of 240 ± 30 g were administered: (GI) placebo, (GII) 2.5 mL of diazepam (GIII) 0.7 mL of Banisteriopsis caapi solution, (GIV) 0,7 mL of Psychotria viridis solution. Groups GV, GVI and GVII received 0.7 mL, 3.5 mL and 7.0 mL of binomio ayahuasca solution, respectively. Main outcome measures. Macroscopically: reflex mov...

This study aimed to characterize the chemical components of essential oil of Citrus sinensis L., determine its antibacterial activity and make dosageforms, mainly for local use. The essential oil was obtained from the dried leaves of the selected species, using a hydrodistillation system with water steam sweep; tests were conducted miscibility and determination of physical constants, such as specific gravity, refractive index and optical rotation. The chemical composition was determined by Gas Chromatography/Mass Spectrometry (CG/EM), standing out in the sugiero: chromatogram spectral signals in the elucidating: Limonene, beta-linalool, decanal and 2 (10)-pinene(1S,5S)-(-)-. The determination of antibacterial activity in vitro was tested by agar diffusion method and the formation of inhibition halos against strains of Gram (+), as Staphylococcus aureus ATCC 25,933 and Staphylococcus epid...

This study aimed to characterize the chemical components of essential oil of Citrus sinensis L., determine its antibacterial activity and make dosageforms, mainly for local use. The essential oil was obtained from the dried leaves of the selected species, using a hydrodistillation system with water steam sweep; tests were conducted miscibility and determination of physical constants, such as specific gravity, refractive index and optical rotation. The chemical composition was determined by Gas Chromatography/Mass Spectrometry (CG/EM), standing out in the sugiero: chromatogram spectral signals in the elucidating: Limonene, beta-linalool, decanal and 2 (10)-pinene(1S,5S)-(-)-. The determination of antibacterial activity in vitro was tested by agar diffusion method and the formation of inhibition halos against strains of Gram (+), as Staphylococcus aureus ATCC 25,933 and Staphylococcus epid...

The aim of the study was to evaluate the antioxidant and antiphotoaging effects of extracts of brown alga Macrocystis integrifolia Bory and develop a dermocosmetic form. From this extracts, was designed the dermocosmetic form in concentrations of 1, 3 and 5%, on a base made of lard with incorporation of beeswax and sesame oil to a pH of 6,5. The antioxidant activity was determined by in vitro methods as CDP and ABTS. The evaluation of antiphotoaging effect in vivo was performed using 25 albino female mice of Mus musculus specie, strain Bald / C53, who were divided into five groups of five mice each; except the group that served as blank, control and intervention groups were depilated hair back and subjected to UVB irradiation for 7 days, morning and night. The in vitro antioxidant activity assay showed that between extracts from stem, foliage and bulb of this alga, there are differences ...

El objetivo del estudio fue evaluar el efecto antioxidante y antifotoenvejecimiento de extractos de la alga parda Macrocystis integrifolia Bory y elaborar una forma dermocosmética. Obtenidos los extractos, se diseñó una forma dermocosmética en concentraciones de 1, 3 y 5%, elaboradas sobre una base de manteca de cerdo con incorporación de cera de abejas y aceite de sésamo a un pH de 6,5. La actividad antioxidante in vitro se determinó por lo métodos CDP y ABTS. La evaluación del efecto antifotoenvejecimiento in vivo se realizó empleando 25 ratones albinos hembra de la especie Mus musculus, cepa Bald/C53, que fueron distribuidos en cinco grupos de cinco ratones cada uno; a excepción del grupo que sirvió de blanco, el grupo control y los grupos de intervención fueron depilados en el lomo y se sometieron a la irradiación UVB durante 7 días, mañana y noche. El ensayo in vitro...

Was determinated chemical composition of essential oil from Caesalpinia spinosa, obtained by stripping with steam method, with a yield of 0,125% v/w, as well as its antioxidant capacity and antibacterial effect against Streptococcus mutans ATCC 35668. To identify chemical constituents were employed Gas Chromatography and Mass Spectrometry (GC/MS), finding 23 compounds, being in greater quantity: pelargonaldehído (11,21%); dihydro-neoclovene-(i) (5,51%); naphthalene,1,2,3,5,6,8a-hexahydro-4,7-dimethyl-1-(1-methylethyl)-,(15-CIS)- (4,87%) y dihydro-cis-alfa-copaene-8-ol (4,13%). Evaluation of antioxidant activity was performed using the methods of 2,2-diphenyl-1-picrylhydrazyl (DPPH) and acid radical 2,2'-azino-bis- (3-etilbenzotiazolin-6-sulfonic acid) (ABTS—⁺), determining that IC50 to both methods was > 200 µL/mL, using as captation reference trolox®, which had IC50 3,8 µg/mL....

Se determinó la composición química del aceite esencial de Caesalpinia spinosa, obtenido por el método de destilación por arrastre con vapor de agua con un rendimiento de 0,125% v/p, así como su capacidad antioxidante y actividad antibacteriana frente a Streptococcus mutans ATCC 35668. Para la identificación de los constituyentes químicos se emplearon Cromatografía de Gas y Espectrometría de Masas (CG/EM), encontrándose 23 compuestos, de los cuales destacan en mayor porcentaje: pelargonaldehído (11,21%); dihydro-neoclovene-(i) (5,51%); naphthalene,1,2,3,5,6,8a-hexahydro-4,7-dimethyl-1-(1-methylethyl)-,(15-CIS)- (4,87%) y dihydro-cis-alfa-copaene-8-ol (4,13%). La evaluación de la actividad antioxidante se realizó aplicando los métodos de 2,2–difenil-1-picrilhidracilo (DPPH) y del radical ácido 2,2’-azino-bis-(3-etilbenzotiazolin-6-sulfónico) (ABTS—⁺), determinándo...