This experimental study was to evaluate the effect of prednisone on the ratio of the length of tooth movement in rats (Rattus norvegicus). The evaluation of this relationship was performed in 20 male Wistar rats 12 weeks of age, weighing 225 ± 25 g, to which adapted a orthodontic breaker in their upper central incisors, were then randomly divided into two groups: control (physiological saline to 2 mL/kg) and experimental (prednisone 20 mg/kg ip), the treatment began five days before placement of the device and then to the first, third and fifth day. With a gauge, was measured the interincisisor distance to finish placing the device, as so as to the first, third, fifth and seventh day. On the 1st day of testing, the control group and the experimental group had a interincisiva distance 1,13 ± 0,18 mm and 0,24 ± 0,09 mm and the 7th day was 1,76 ± 0,14 mm and 1,56 ± 0,20 mm, respectivel...

This study aimed to demonstrate the estrogenic properties of the hydroalcoholic extract of Medicago sativa L (alfalfa) in albino rats ovariectomized (OVX). Were used 48 albino Sprague Dawley rats of 200 to 250 g, 8 weeks of age, of whom 40 underwent surgical removal of both ovaries, following the technique of bilateral ovariectomy on the flank. After the post-operative were divided into six groups and hydroalcoholic extract was administered orally under the following scheme of work: Group 1 negative control: (OVX), vehicle of the extract 2mL/kg; Group 2 positive control: (OVX), estradiol subcutaneous 3 µg/kg; Group 3, 4 y 5: (OVX) more extract 100, 500 and 1000 mg/kg, respectively; and Group 6: not OVX control the surgical procedure (SHAM). The treatment lasted 14 days and was evaluated the body weight, uterine weight, changes of oestrus by vaginal smears and hormonal profile analysis. ...

This experimental study was to evaluate the effect of prednisone on the ratio of the length of tooth movement in rats (Rattus norvegicus). The evaluation of this relationship was performed in 20 male Wistar rats 12 weeks of age, weighing 225 ± 25 g, to which adapted a orthodontic breaker in their upper central incisors, were then randomly divided into two groups: control (physiological saline to 2 mL/kg) and experimental (prednisone 20 mg/kg ip), the treatment began five days before placement of the device and then to the first, third and fifth day. With a gauge, was measured the interincisisor distance to finish placing the device, as so as to the first, third, fifth and seventh day. On the 1st day of testing, the control group and the experimental group had a interincisiva distance 1,13 ± 0,18 mm and 0,24 ± 0,09 mm and the 7th day was 1,76 ± 0,14 mm and 1,56 ± 0,20 mm, respectivel...

El presente estudio tuvo como objetivo demostrar las propiedades estrogénicas del extracto hidroalcohólico de Medicago sativa L (alfalfa) en ratas albinas ovariectomizadas (OVX). Se utilizaron 48 ratas albinas Sprague Dawley, hembras, de 200 a 250 g, de 8 semanas de edad, de las cuales 40 fueron sometidas a extirpación quirúrgica de ambos ovarios siguiendo la técnica de ovariectomía bilateral por el flanco. Luego del post-operatorio se dividieron en seis grupos y se administró el extracto hidroalcohólico por vía oral bajo el siguiente esquema de trabajo: Grupo 1 control negativo: (OVX), vehículo del extracto 2mL/kg; Grupo 2 control positivo: (OVX), estradiol subcutáneo 3µg/kg; Grupo 3, 4 y 5: (OVX) más extracto a 100, 500 y 1000 mg/kg, respectivamente; y Grupo 6: No OVX, control del procedimiento quirúrgico (SHAM). El tratamiento duró 14 días y se evaluó el peso corporal...

Objective of this experimental study was to determine the antihypertensive and toxic activities of methanolic extract from Calceolaria myriophylla (zapatilla). Male albino mice Mus musculus BALB/c of two months, 25±5 g weigth, were used for induction of hypertension with N-nitro-L-arginine methyl ester (L-NAME) administered at doses of 130 mg/kg/day, orally for seven days, measuring baseline and post induction blood pressure. The mice were divided into six groups of ten animals: negative control (distilled water), positive control (L-NAME), experimental groups at doses of 200, 400 and 600 mg/kg of methanolic extract, and enalapril group to doses 75 mg/kg. The treatment was administered for 30 days continuous and blood pressure was measured every five days. The study of chronic toxicity was determined to repeated doses for 60 days in normotensive rats, divided into three groups of six an...

El presente estudio experimental tuvo como objetivo determinar las actividades antihipertensiva y tóxica del extracto metanólico de Calceolaria myriophylla (zapatilla). Se utilizaron ratones albinos machos Mus musculus BALB/c, de dos meses con peso de 25±5 gramos para la inducción de hipertensión arterial con la administración de N-nitro-L-arginina metíl eter (L-NAME) a dosis de 130 mg/kg/día, vía oral durante siete días, midiéndose la presión arterial basal, y post inducción. Los ratones fueron divididos en seis grupos de diez animales: grupo control negativo (agua destilada), control positivo (L-NAME), grupos experimentales a dosis de 200, 400 y 600 mg/kg del extracto metanólico, y enalapril a 75 mg/kg. Se administró el tratamiento durante 30 días continuos, midiendo la presión arterial cada cinco días. El estudio de toxicidad crónica se determinó a dosis repetidas ...

This study aimed to determine the effect of ethanol extract of Calceolaria myriophylla "shoe" in the metabolic syndrome induced by fructose in rats. Was made qualitative phytochemical analysis and used 48 male albino rats of race Holtzmann 2 months old with a weight fluctuated between 180 and 200 g, was performed induction of metabolic syndrome in rats by oral administration of fructose to 60% for 90 days and work according to the following design: six groups of eight animals each group divided into control group, fructose group, fructose group more ethanolic extract (03 groups at doses of 200, 400 and 600 mg/kg, respectively and fructose more drugs (atorvastatin 20 mg/kg group and 20 mg captopril mg/kg in combination), treatments were administered orally for 90 days. Were observed decreased levels glucose, lipid profile (total cholesterol, HDL-cholesterol and triglycerides), systolic an...

The present study aimed to determine the effect of the essential oil of oregano (Origanum vulgare L.) on the external otitis in dogs by Malassezia spp. Were used 38 animals, 22 had bilateral otitis and 16 unilateral (60 ears). Were collected the ear discharge of both ears using sterile swabs and samples were evaluated by performing yeast count per field, after had been subjected to Wright staining. Were made stable dilutions of essential oil of oregano in mineral oil at 1, 2 and 5%. The evaluation of the antifungal effect in vivo was determined by comparing the three dilutions with clotrimazole 1%, used as positive control, and with pure mineral oil in placebo group. The effectiveness of the effect of the dilutions was compared by counting yeasts visual field to the 3rd and 8th day of treatment. Got Better effect to the 8th day with the essential oil of oregano (AEO) 5% with 75% of ears ...

El presente estudio tuvo como objetivo determinar el efecto del extracto etanólico de Calceolaria myriophylla “zapatilla” en el síndrome metabólico inducido por fructosa en ratas. Se realizó el análisis fitoquímico cualitativo y se utilizó 48 ratas albinas machos de raza Holtzmann de 2 meses de edad con un peso que fluctuó entre 180 a 200 g. Se realizó la inducción del síndrome metabólico en ratas mediante la administración, vía oral, de fructosa al 60% durante 90 días y se trabajo según el siguiente diseño: seis grupos de ocho animales cada uno, divididos en grupo control, grupo fructosa, grupo fructosa más extracto etanólico (03 grupos a dosis de 200, 400 y 600 mg/kg, respectivamente y fructosa más medicamentos (atorvastatina 20 mg/kg y captopril 20 mg/kg en combinación); los tratamientos se administraron vía oral por 90 días. Se observo una disminución de l...

El presente estudio tuvo como objetivo determinar el efecto del aceite esencial de orégano (Origanum vulgare L.) sobre la otitis externa por Malassezia spp. en perros. Se emplearon 38 animales, 22 de los cuales presentaron otitis bilateral y 16 otitis unilateral (60 orejas). Se colectó la secreción ótica de ambos oídos con ayuda de hisopos estériles y las muestras se evaluaron efectuando el conteo de levaduras por campo visual después de haber sido sometidas a tinción Wright. Se realizaron diluciones estables del aceite esencial de orégano en aceite mineral al 1, 2 y 5%. La evaluación del efecto antifúngico in vivo se realizó comparando las tres diluciones con clotrimazol al 1% −utilizado como control positivo− y con aceite mineral puro en el grupo placebo. La eficacia del efecto de las diluciones se comparó mediante el conteo de levaduras por campo visual al 3er y 8vo d...

This study was designed in three phases: 1. Preliminary assessment of the extract of calyces. 2. Formulation and assessment of gels incorporating the extract. 3. Assessment of the anti-inflammatory activity of the gel. Objective. To evaluate the anti-inflammatory activity of a gel based on the lyophilized ethanolic extract of calyces of Physalis peruviana “goldenberry”. Methodology. Calyces of goldenberry collected from a processing company in the department of Cuzco were subject of a reflux extraction with petroleum ether and, from the residue, it was prepared an ethanolic extract, by maceration, which was lyophilized and incorporated at concentration of 1 % in different formulations of gel base of sodium carboxymethylcellulose (CMC), polyacrylamide and carbomer. Those preparations that presented better physical compatibility were subjected to preliminary stability. For the assessme...

Este estudio se diseñó en tres etapas: 1. Evaluación preliminar del extracto de cálices. 2. Formulación y evaluación de geles incorporando el extracto. 3. Evaluación de la actividad antiinflamatoria del gel. Objetivo. Evaluar la actividad antiinflamatoria de un gel a base de extracto etanólico liofilizado de cálices de Physalis peruviana “aguaymanto”. Metodología. Cálices de aguaymanto, obtenidos de una empresa procesadora en Cuzco fueron sometidos a extracción por reflujo con éter de petróleo y, a partir del residuo se preparó un extracto etanólico por maceración, que fue liofilizado e incorporado a concentración de 1 % en diversas formulaciones en gel base de carboximetilcelulosa sódica (CMC), poliacrilamida y carbómero. Aquellas preparaciones que presentaron mejor compatibilidad física fueron sometidas a estabilidad preliminar. Para la evaluación de la activi...

Were studied the antioxidant effect and toxicity of the hot springs of San Antonio de Putina-Puno. Were used methods of lipid antiperoxidation, malondialdehyde (expressed as TBARS), in vivo oral acute toxicity at fixed dose according to 423 OECD and the physical chemistry composition by inductively coupled plasma source mass spectrometry (ICP-MS). The antioxidant effect presented significant difference between control group (0,0527 ŋm) and the study group (0,0390 ŋm) and malondialdehyde (p<0,05) and in vivo oral acute toxicity at fixed-dose treatment. Not evident signs of intoxication, moreover, in the composition of these hot springs were found: chloride 71,9%, sodium 80,3 % and sulphate 22,7% meq. Was concluded that the thermal mineral hot springs, are not toxic, have antioxidant effect and are chlorinated, sulphate and sodium type.

Se estudiaron el efecto antioxidante y la toxicidad de las aguas termales de San Antonio de Putina-Puno, por los métodos de antiperoxidación lipídica, malondialdehido (expresado como TBARS), la toxicidad aguda oral in vivo a dosis fijas según la OECD 423 y la composición fisicoquímica por espectrometría de pesos con fuente de plasma de acoplamiento inductivo (ICP-MS). El efecto antioxidante presentó diferencia significativa entre el grupo control (0,0527 ŋm) y el grupo estudio (0,0390 ŋm) malondialdehido (p<0,05) y en la toxicidad aguda oral in vivo a dosis fijas, después del tratamiento, no se evidenciaron signos de intoxicación, además en la composición de dichas aguas termales se encontraron cloruro 71,9%, sodio 80,3% y sulfato 22,7% meq. Se concluye que las aguas termales mineromedicinales, son atóxicas, tienen efecto antioxidante y son de tipo clorurada, sulfatada ...

The objective of the study was to determine the healing effect of Colletia spinosissima (tacsana) shampoo based on induced injuries in mouse skin. Were used 36 albino mice, weighing between 30-35 g; to which was depilated in the dorsal area, applying lidocaine gel 4% in the depilated area to induce lesions (wounds) on the skin. The animals were allocated into five groups, each group consisting of six mice. The experimental design was composed as follows: G1: negative control, G2: positive control; G3, G4 and G5 formulated 10%; 20%; and 30%, respectively; G6: Pantene®. The liquid shampoo was administered twice daily for fourteen consecutive days. Results shows that in groups 4 and 5 was reduced the injury to the skin on the fourth day, the animals of group 5 exhibited a process of scabs and skin repair, with collagen formation in the histological study. It is concluded that liquid based ...

El objetivo del estudio fue determinar el efecto cicatrizante del champú a base de Colletia spinosissima (tacsana) en lesiones inducidas en piel del ratón. Se utilizaron 36 ratones albinos, de peso entre 30-35 g, a los que se depiló en la zona dorsal, aplicándose lidocaína gel al 4% en la zona depilada para inducir lesiones (heridas) en la piel. Los animales fueron asignados en cinco grupos, cada uno constituido por seis ratones. El diseño experimental estuvo conformado de la siguiente manera: G1: Control negativo; G2: Control positivo; G3, G4 y G5: formulado al 10%; 20%; y 30%, respectivamente; G6: Pantene®. Se administró el champú líquido dos veces al día, por catorce días consecutivos. En los resultados se observó en los grupos 4 y 5 disminución de la lesión sobre la piel al cuarto día, los animales del grupo 5 presentaron un proceso de costras y reparación de la piel...