Resultados de Búsqueda por Autor

1

artículo

Aprendizaje Basado en Proyectos (ABPy) en la asignatura Acuicultura de la Universidad Nacional Agraria La Molina

Publicado por
Verástegui M., A. S., Arias C., J.

Publicado 2016

El objetivo del presente trabajo fue evaluar la metodología ABPy en el desarrollo de la parte práctica de la asignatura Acuicultura II de la carrera de Pesquería de la Universidad Nacional Agraria La Molina (UNALM), durante el semestre 2013-I. El desarrollo de la metodología en la asignatura fue realizado con dos grupos horarios de trabajo, donde los estudiantes condujeron dos experimentos de crianza de tilapia (Oreochromis niloticus) con similares requerimientos de dedicación académica práctica en el aspecto relacionado al manejo de los peces, desde el acondicionamiento de los tanques de crianza hasta la presentación oral de los informes finales al final del semestre académico. Los resultados de los instrumentos de medición, indican que el mayor porcentaje (62.07 por ciento) de estudiantes logró una calificación de muy bueno en el rubro de práctica, con proyectos d...

2

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Uso de la harina de Maca (Lepidium peruvianum G. Chacón) como insumo de la dieta de alevines de tilapia roja (Oreochromis spp.), criadas en condiciones de laboratorio

Publicado por
Verástegui M., Aníbal, Vargas C., Jessie, Loo K., Teddy

Publicado 2009

Enlace

En el presente trabajo se evaluó la inclusión de harina pre-gelatinizada de maca (Lepidium peruvianum G. Chacón) en términos de supervivencia, tasa de crecimiento (TC), conversión alimenticia (CA) y coeficiente de digestibilidad aparente de la materia seca de la dieta (CDA). Se formularon 4 dietas isoprotéicas e isocalóricas, las cuales fueron suplementadas con 4 niveles de harina de maca pre-gelatinizada: 0 (dieta testigo, T1), 5 (dieta T2), 10 (dieta 3), 15% (dieta 4) cada uno realizado por triplicado. El experimento se llevó a cabo en la Universidad Nacional Agraria La Molina (UNALM), en el Laboratorio de Acuicultura de la Facultad de Pesquería, en un sistema de recirculación de agua, durante 12 semanas. Se observó que con un nivel de inclusión de harina de maca pre-gelatinizada del 5% se puede mejorar la utilización del alimento en términos de crecimiento sin lle...

3

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Observaciones preliminares del efecto de la Canela (Cinnamomum zeylanicum) y Clavo de olor (Eugenia caryophyllata) en el crecimiento de juveniles de Tilapia Roja (Oreochromis spp.)...

Publicado por
Verastegui M., Aníbal, Fukushima, Manuel

Publicado 2009

Enlace

El experimento se realizó para determinar el efecto promotor del crecimiento de la canela (Cinnamomum zeylanicum) y clavo de olor (Eugenia caryophyllata) incorporados al 0.5, 1.0 y 2.0 % en base a peso, en la dieta de juveniles de tilapia roja (Oreochromis spp.). Se preparó 7 dietas experimentales, 3 tratamientos por cada prebiótico natural (canela y clavo de olor) y uno control. La alimentación se efectuó tres veces al día (8:00, 12:00 y 16:00 horas) en juveniles de 7.71 ± 0.23 g. (7.84 ± 0.09 cm. LT). El periodo experimental comprendió 8 semanas, utilizando un sistema de recirculación (RAS). Los peces alimentados con canela a un nivel del 2 % mostraron el mayor crecimiento. El tratamiento que muestra el 2do valor mas alto en crecimiento fue el que corresponde al mismo producto incluido al 1.0 % en peso. Lo que demuestra que ambos niveles de inclusión de este promotor ...

4

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Protein requirement in juveniles of the marine fish Chita Anisotremus scapularis (Tschudi, 1846) (Perciformes: Haemulidae)

Publicado por
Yanac P., Ciro, Verástegui M., Aníbal, Sotelo-Mendez, Alejandrina, Hall, Steven

Publicado 2023

Enlace

The present study was carried out to determine the protein requirement in Peruvian grunt juvenile, Anisotremus scapularis. In total, 72 fish weighing 19.95 ± 4.09 g and length of 10.31 ± 0.63 cm were used. They were randomly distributed in groups of six units per tank. The experimental diets contained protein levels of 45, 50, 55 and 58%, which were supplied to three experimental units per diet. The fish were fed three times a day to satiety for a period of 56 days. The results showed that final weight, weight gain, feed intake and feed conversion ratio were significantly (p<0.05) affected by dietary protein levels. However, the increase in size and the specific growth rate did not present significant differences (p>0.05) between treatments. The protein requirement based on weight gain using second degree polynomial regression was 56.78%, for optimal growth of Peruvian grunt juve...

5

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Human hydatidosis in the central Andes of Peru: Evolution of the disease over 3 years

Publicado por
Moro P.L., Gilman R.H., Verastegui M., Bern C., Silva B., Bonilla J.J.

Publicado 1999

Enlace

This study was funded in part by the National Institutes of Health (grant no. U01-A135894, awarded to The Johns Hopkins University), the RG-ER Fund, and Consejo Nacional de Ciencia y Tecnologı´a (CONCYTEC; Lima, Peru).

6

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Screening for cystic echinococcosis in an endemic region of Peru using portable ultrasonography and the enzyme-linked immunoelectrotransfer blot (EITB) assay

Publicado por
Moro P.L., Garcia H.H., Gonzales A.E., Bonilla J.J., Verastegui M., GilmanMD R.H.

Publicado 2005

Enlace

Financial support for this study was received from Consejo Nacional de Ciencia y Tecnología (CONCYTEC), Lima, Peru. The methods used in the present study comply with current Peruvian law governing the participation of human subjects in biomedical research.

7

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Changes in inflammatory gene expression in brain tissue adjacent and distant to a viable cyst in a rat model for neurocysticercosis

Publicado por
Carmen-Orozco R.P., Dávila-Villacorta D.G., Delgado-Kamiche A.D., Celiz R.H., Trompeter G., Sutherland G., Gavídia C., Garcia H.H., Gilman R.H., Verástegui M.R., Cysticercosis Working Group in Peru

Publicado 2021

Enlace

Background The parasite Taenia solium causes neurocysticercosis (NCC) in humans and is a common cause of adult-onset epilepsy in the developing world. Hippocampal atrophy, which occurs far from the cyst, is an emerging new complication of NCC. Evaluation of molecular pathways in brain regions close to and distant from the cyst could offer insight into this pathology. Methods Rats were inoculated intracranially with T. solium oncospheres. After 4 months, RNA was extracted from brain tissue samples in rats with NCC and uninfected controls, and cDNA was generated. Expression of 38 genes related to different molecular pathways involved in the inflammatory response and healing was assessed by RT-PCR array. Results Inflammatory cytokines IFN-?, TNF-?, and IL-1, together with TGF-? and ARG-1, were overexpressed in tissue close to the parasite compared to non-infected tissue. Genes for IL-1A, CS...

8

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In vitro model of postoncosphere development, and in vivo infection abilities of Taenia solium and Taenia saginata

Publicado por
Palma S., Chile N., Carmen-Orozco R.P., Trompeter G., Fishbeck K., Cooper V., Rapoport L., Bernal-Teran E.G., Condori B.J., Gilman R.H., Verastegui M.R., for the Cysticercosis Working Group in Peru

Publicado 2018

Enlace

Taenia solium is known to cause human cysticercosis while T. saginata does not. Comparative in vitro and in vivo studies on the oncosphere and the postoncospheral (PO) forms of T. solium and T. saginata may help to elucidate why cysticercosis can occur from one and not the other. The aim of this study was to use in vitro culture assays and in vivo models to study the differences in the development of the T. solium and T. saginata oncosphere. Furthermore, this study aimed to evaluate the expression of cytokines and metalloproteinases (MMPs) in human peripheral blood mononuclear cells (PBMCs), which were stimulated by these oncospheres and PO antigens. T. solium and T. saginata activated oncospheres (AO) were cultured in INT-407 and HCT-8 intestinal cells for 180 days. The T. solium began to die while the T. saginata grew for 180 days and developed to cysticerci in INT-407 cells. Rats were...

9

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Blood–brain barrier disruption and angiogenesis in a rat model for neurocysticercosis

Publicado por
Carmen-Orozco R.P., Dávila-Villacorta D.G., Cauna Y., Bernal-Teran E.G., Bitterfeld L., Sutherland G.L., Chile N., Céliz R.H., Ferrufino-Schmidt M.C., Gavídia C.M., Sterling C.R., García H.H., Gilman R.H., Verástegui M.R.

Publicado 2019

Enlace

Neurocysticercosis (NCC) is a helminth infection affecting the central nervous system caused by the larval stage (cysticercus) of Taenia solium. Since vascular alteration and blood–brain barrier (BBB) disruption contribute to NCC pathology, it is postulated that angiogenesis could contribute to the pathology of this disease. This study used a rat model for NCC and evaluated the expression of two angiogenic factors called vascular endothelial growth factor (VEGF-A) and fibroblast growth factor (FGF2). Also, two markers for BBB disruption, the endothelial barrier antigen and immunoglobulin G, were evaluated using immunohistochemical and immunofluorescence techniques. Brain vasculature changes, BBB disruption, and overexpression of angiogenesis markers surrounding viable cysts were observed. Both VEGF-A and FGF2 were overexpressed in the tissue surrounding the cysticerci, and VEGF-A was o...

10

artículo

The immunoglobulin M-Shed acute phase antigen (SAPA)-test for the early diagnosis of congenital Chagas disease in the time of the elimination goal of mother-to-child transmission

Publicado por
Castro-Sesquen Y.E., Tinajeros F., Bern C., Galdos-Cardenas G., Malaga E.S., Valencia Ayala E., Hjerrild K., Clipman S.J., Lescano A.G., Bayangos T., Castillo W., Menduiña M.C., Talaat K.R., Gilman R.H., Verastegui M., Calderon M., Chávez C., Leigue J.K., Hinojosa E., Urquizu F., Gorena M., Serrudo V., Cabrera L., Romero Y.K., Chagas Working Group in Bolivia and Peru

Publicado 2021

Enlace

The work was supported by Fondo Nacional de Desarrollo Cientifico, Tecnologico y de Innovacion Tecnologica, FONDECYT, Peru (N084-2016) to Y. E. C.-S.; the National Institutes of Health (R01-AI87776 and D43-TW010074 to R. H. G. and D43 TW007393 to A. G. L.); and InBios International, Inc to R. H. G. through the Johns Hopkins Bloomberg School of Public Health.

11

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Antibody Banding Patterns of the Enzyme-Linked Immunoelectrotransfer Blot and Brain Imaging Findings in Patients with Neurocysticercosis

Publicado por
Arroyo G., Rodriguez S., Lescano A.G., Alroy K.A., Bustos J.A., Santivañez S., Pretell E.J., Garcia H.H., Gilman R.H., Gonzalez A.E., Tsang V.C.W., Gonzalez I., Saavedra H., Martinez M., Alvarado M., Verastegui M., Zimic M., Mayta H., Guerra C., Castillo Y., Castro Y., Lopez M.T., Gavidia C.M., Gomez L., Moyano L.M., Gamboa R., Muro C., Vilchez P., Nash T.E., Mahanty S., Noh J., Handali S., Friedland J.

Publicado 2018

Enlace

This work was partially supported by the Fogarty International Center/NIH (training grant numbers D43TW001140 and D43TW007393) and by the National Council for Science, Technology and Innovation of Peru (CONCYTEC).

12

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Use of magnetic particles in the purification of igm antibodies against taenia solium

Publicado por
Perez L.A., Castillo Y., Espinoza C., Toribio L.M., Santos Y., Martel K.S., Wilkins P.P., Bustos J.A., García H.H., Castro-Sesquen Y.E., Gilman R.H., Gonzalez A.E., Tsang V.C.W., Rodriguez S., Gonzalez I., Saavedra H., Sanchez S., Martinez M., Verastegui M., Zimic M., Santivañez S., Mayta H., Pajuelo M., Arroyo G., Lopez M.T., Gomez L., Vargas A., Gavidia C.M., Moyano L.M., Gamboa R., Muro C., Vichez P., O´neal S., Handali S., Noh J., Nash T.E., Mahanty S., Friedland J., Grupo de Trabajo en Cisticercosis en Peru

Publicado 2020

Enlace

The use of L protein coupled magnetic particles for the concentration and purification of immunoglobulin M (mIgM) monoclonal antibodies against Taenia solium was evaluated. Three concentration methods and different elution times were evaluated and the ratio of particles to the ratio of mIgM was optimized. It is demonstrated that: 1) with the use of magnetic particles, a previous concentration of mIgM is not required, which reduces the manipulation of the antibodies and improves the recovery, 2) the use of a binding buffer can be omitted, since the pH of most cell culture supernatants are neutral, and 3) longer elution times (~ 45 minutes) are needed to increase recovery to a level greater than 80%. The study demonstrates that the use of L protein-coupled magnetic particles is a simple and efficient tool for mIgM concentration and purification. © 2020, Instituto Nacional de Salud. All ri...