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artículo

The immunoglobulin M-Shed acute phase antigen (SAPA)-test for the early diagnosis of congenital Chagas disease in the time of the elimination goal of mother-to-child transmission

Publicado por
Castro-Sesquen Y.E., Tinajeros F., Bern C., Galdos-Cardenas G., Malaga E.S., Valencia Ayala E., Hjerrild K., Clipman S.J., Lescano A.G., Bayangos T., Castillo W., Menduiña M.C., Talaat K.R., Gilman R.H., Verastegui M., Calderon M., Chávez C., Leigue J.K., Hinojosa E., Urquizu F., Gorena M., Serrudo V., Cabrera L., Romero Y.K., Chagas Working Group in Bolivia and Peru

Publicado 2021

Enlace

The work was supported by Fondo Nacional de Desarrollo Cientifico, Tecnologico y de Innovacion Tecnologica, FONDECYT, Peru (N084-2016) to Y. E. C.-S.; the National Institutes of Health (R01-AI87776 and D43-TW010074 to R. H. G. and D43 TW007393 to A. G. L.); and InBios International, Inc to R. H. G. through the Johns Hopkins Bloomberg School of Public Health.

artículo

Use of magnetic particles in the purification of igm antibodies against taenia solium

Publicado por
Perez L.A., Castillo Y., Espinoza C., Toribio L.M., Santos Y., Martel K.S., Wilkins P.P., Bustos J.A., García H.H., Castro-Sesquen Y.E., Gilman R.H., Gonzalez A.E., Tsang V.C.W., Rodriguez S., Gonzalez I., Saavedra H., Sanchez S., Martinez M., Verastegui M., Zimic M., Santivañez S., Mayta H., Pajuelo M., Arroyo G., Lopez M.T., Gomez L., Vargas A., Gavidia C.M., Moyano L.M., Gamboa R., Muro C., Vichez P., O´neal S., Handali S., Noh J., Nash T.E., Mahanty S., Friedland J., Grupo de Trabajo en Cisticercosis en Peru

Publicado 2020

Enlace

The use of L protein coupled magnetic particles for the concentration and purification of immunoglobulin M (mIgM) monoclonal antibodies against Taenia solium was evaluated. Three concentration methods and different elution times were evaluated and the ratio of particles to the ratio of mIgM was optimized. It is demonstrated that: 1) with the use of magnetic particles, a previous concentration of mIgM is not required, which reduces the manipulation of the antibodies and improves the recovery, 2) the use of a binding buffer can be omitted, since the pH of most cell culture supernatants are neutral, and 3) longer elution times (~ 45 minutes) are needed to increase recovery to a level greater than 80%. The study demonstrates that the use of L protein-coupled magnetic particles is a simple and efficient tool for mIgM concentration and purification. © 2020, Instituto Nacional de Salud. All ri...