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artículo
Publicado 2020
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The use of L protein coupled magnetic particles for the concentration and purification of immunoglobulin M (mIgM) monoclonal antibodies against Taenia solium was evaluated. Three concentration methods and different elution times were evaluated and the ratio of particles to the ratio of mIgM was optimized. It is demonstrated that: 1) with the use of magnetic particles, a previous concentration of mIgM is not required, which reduces the manipulation of the antibodies and improves the recovery, 2) the use of a binding buffer can be omitted, since the pH of most cell culture supernatants are neutral, and 3) longer elution times (~ 45 minutes) are needed to increase recovery to a level greater than 80%. The study demonstrates that the use of L protein-coupled magnetic particles is a simple and efficient tool for mIgM concentration and purification. © 2020, Instituto Nacional de Salud. All ri...
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The genus Pleurotus presents a multivariate species diversity due to its ability to grow in different substrates and environments. Whether wild or cultivated, they are edible mushrooms, as they present a high nutritional value and are medicinal due to their bioactive compounds with positive health effects. The aim of this review is to highlight the importance of the genus Pleurotus, since it is a cosmopolitan mushroom, and its properties can be used in different industrial applications and be a functional alternative for our future. Due to their saprophytic nature, they produce enzymes that act on the substrate in which they grow, degrading lignocellulosic material such as wood, forest and agricultural residues, hardwoods, wood by-products, cereal straw, bagasse, etc., and thanks to this degradative capacity, their enzymes are used in a wide range of biotechnological and environmental ap...
3
artículo
Publicado 2018
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Dr. J. Rodriguez, E. Gushiken por el modelamiento del VHH-ES1. El vector pHEN2 fue donado por el Dr. D. Baty y Dr. D. Kastelic (Protein Biotechnology Group, Babraham Bioscience Technologies, UK). TB realizó una estadía en el Instituto Pasteur, Francia bajo la supervisión del Dr. P. Lafaye. Fuentes de financiamiento: El trabajo fue financiado por el Programa Nacional de Innovación para la Competitividad y Productividad (Innóvate Perú), contrato 123-FINCyT-IA-2013, «Generación de anticuerpos de dominio-único de alpacas para el diagnóstico y tratamiento de enfermedades hepáticas». YA recibió financiamiento por el proyecto Postdoctoral FONDECYT «Generación de Anticuerpos de dominio único de alpacas para el diagnóstico y tratamiento de enfermedades hepáticas», EF015-2013.
