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artículo
PRODUCCIÓN IN VITRO DE BRADIZOÍTOS DE NEOSPORA CANINUM #
Publicado por
Risco Castillo y Cols., Verónica
Publicado 2019 Enlace
Neospora caninum is a parasite identified as a major cause of abortion in cattle and neurological disease in various animal species. It is closely related to Toxoplasma gondii, sharing the ability to persist indefinitely in latent stage within the host as a tissue cyst containing slow-dividing bradyzoites. Here we compared different stress methods to induce in vitro bradyzoite conversion, using MARC-145 cells infected with Nc-Liverpool isolate. The tachyzoite-to-bradyzoite conversion rate was monitored at days 3, 5, and 7 post-stress in a double immunofluorescence assay using a mAb against the tachyzoite antigen SAG1 (aSAG1) and a rabbit serum directed to the intracytoplasmatic bradyzoite antigen BAG1 (aBAG1). Seven-day treatment with sodium nitroprusside 70 µM, offered the highest bradyzoite transformation rate and the best yield of total parasitophorous vacuoles observed. In the prese...
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2
artículo
FORMACIÓN DE UN COMPUTER SECURITY INCIDENT RESPONSE TEAM EN LA UNIVERSIDAD NACIONAL DE INGENIERIA CSIRT-UNI
Publicado por
Borda Castillo y Cols., Erik J.
Publicado 2019 Enlace
The present work consists of proposing the formation of a Computer Security Incident Response Team in the National University of Engineering, which had to respond of effective and opportune way to determined incident of computer security of our university and some sectors of the society. Also, this denominated initiative CSIRT-UNI looks for to impel actively and to comprise of the Peruvian’s Coordination Center / Computer Emergency Response Team denominated PERUCERT/CC.
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3
artículo
Quantification of the Carbon and Biomass Content of a Remaining Forest of Polylepis Spp. In the Peruvian Andes.
Publicado por
Cordova Cordova, O.R, Castañeda Tinco. M.I., Alomia Lucero, J.M., Rivera Campano, Milko Raul, Alvarez Montalvan, C.E., Rojas Castillo, Y.
Publicado 2024 Enlace
The objective of this research was to quantify the contents of aboveground biomass and carbon of Polylepis spp. “queñoa”, in a relict forest of the Muylaque countryside community in the department of Moquegua between 3,700 and 4,300 m.a.s.l. Under a forest inventory, dasometric parameters (stem diameter, crown diameter, total tree height) were recorded in 20 × 20 m plots, following MINAM (Peruvian Ministry of the Environment) guidelines, to then calculate biomass through the application of an allometric equation for Polylepis and the conversion factor obtained from samples of 20 individuals with the photometric method to find the stored carbon. The laboratory results showed that the conversion factor from biomass to stored carbon was 0.58. In addition, using the information per plot in the forest inventory and applying the allometric equation, the expansion factor per hectare a...
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4
artículo
Quantification of the Carbon and Biomass Content of a Remaining Forest of Polylepis Spp. In the Peruvian Andes.
Publicado por
Cordova Cordova, O.R, Castañeda Tinco. M.I., Alomia Lucero, J.M., Rivera Campano, Milko Raul, Alvarez Montalvan, C.E., Rojas Castillo, Y.
Publicado 2024 Enlace
The objective of this research was to quantify the contents of aboveground biomass and carbon of Polylepis spp. “queñoa”, in a relict forest of the Muylaque countryside community in the department of Moquegua between 3,700 and 4,300 m.a.s.l. Under a forest inventory, dasometric parameters (stem diameter, crown diameter, total tree height) were recorded in 20 × 20 m plots, following MINAM (Peruvian Ministry of the Environment) guidelines, to then calculate biomass through the application of an allometric equation for Polylepis and the conversion factor obtained from samples of 20 individuals with the photometric method to find the stored carbon. The laboratory results showed that the conversion factor from biomass to stored carbon was 0.58. In addition, using the information per plot in the forest inventory and applying the allometric equation, the expansion factor per hectare a...
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5
artículo
Antibody Banding Patterns of the Enzyme-Linked Immunoelectrotransfer Blot and Brain Imaging Findings in Patients with Neurocysticercosis
Publicado por
Arroyo G., Rodriguez S., Lescano A.G., Alroy K.A., Bustos J.A., Santivañez S., Pretell E.J., Garcia H.H., Gilman R.H., Gonzalez A.E., Tsang V.C.W., Gonzalez I., Saavedra H., Martinez M., Alvarado M., Verastegui M., Zimic M., Mayta H., Guerra C., Castillo Y., Castro Y., Lopez M.T., Gavidia C.M., Gomez L., Moyano L.M., Gamboa R., Muro C., Vilchez P., Nash T.E., Mahanty S., Noh J., Handali S., Friedland J.
Publicado 2018 Enlace
This work was partially supported by the Fogarty International Center/NIH (training grant numbers D43TW001140 and D43TW007393) and by the National Council for Science, Technology and Innovation of Peru (CONCYTEC).
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6
artículo
Use of magnetic particles in the purification of igm antibodies against taenia solium
Publicado por
Perez L.A., Castillo Y., Espinoza C., Toribio L.M., Santos Y., Martel K.S., Wilkins P.P., Bustos J.A., García H.H., Castro-Sesquen Y.E., Gilman R.H., Gonzalez A.E., Tsang V.C.W., Rodriguez S., Gonzalez I., Saavedra H., Sanchez S., Martinez M., Verastegui M., Zimic M., Santivañez S., Mayta H., Pajuelo M., Arroyo G., Lopez M.T., Gomez L., Vargas A., Gavidia C.M., Moyano L.M., Gamboa R., Muro C., Vichez P., O´neal S., Handali S., Noh J., Nash T.E., Mahanty S., Friedland J., Grupo de Trabajo en Cisticercosis en Peru
Publicado 2020 Enlace
The use of L protein coupled magnetic particles for the concentration and purification of immunoglobulin M (mIgM) monoclonal antibodies against Taenia solium was evaluated. Three concentration methods and different elution times were evaluated and the ratio of particles to the ratio of mIgM was optimized. It is demonstrated that: 1) with the use of magnetic particles, a previous concentration of mIgM is not required, which reduces the manipulation of the antibodies and improves the recovery, 2) the use of a binding buffer can be omitted, since the pH of most cell culture supernatants are neutral, and 3) longer elution times (~ 45 minutes) are needed to increase recovery to a level greater than 80%. The study demonstrates that the use of L protein-coupled magnetic particles is a simple and efficient tool for mIgM concentration and purification. © 2020, Instituto Nacional de Salud. All ri...
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