Resultados de Búsqueda por Autor

1

artículo

Perilesional brain edema and seizure activity in patients with calcified neurocysticercosis.

Publicado por
Nash, Theodore E., Pretell, E. Javier, Lescano, Andres G., Bustos, Javier A., Gilman, Robert H., Gonzalez, Armando E., Garcia, Héctor H., The Cysticercosis Working Group in Peru

Publicado 2008

Enlace

Estudio de cohorte prospectivo con un sub estudio de control de caso anidado, destinado a evaluar la incidencia de recaídas de convulsiones y su asociación con edema perilesional en pacientes con cisticercosis calcificada. Se incluyeron 17 pacientes de 65 años, atendidos en el Instituto Nacional de Ciencias Neurológicas, en Lima, Perú, con Neurocisticercosis y una historia de convulsiones y dolores de cabeza severos recurrentes dentro de los 10 años previos.

2

artículo

Identification and culture of proliferative cells in abnormal taenia solium larvae: Role in the development of racemose neurocysticercosis

Publicado por
Orrego, Miguel A., Verastegui, Manuela R., Vasquez, Carlos M., Koziol, Uriel, Laclette, Juan P., Garcia, Hector H., Nash, Theodore E.

Publicado 2021

Enlace

This work was supported by National Council for Science, Technology, and Technological Innovation (CONCYTEC) – World Bank, through its executing unit National Fund for the Development of Science, Technology, and Technological Innovation (FONDECYT) contract [E033-01-08-2018-FONDECYT/Banco Mundial Programas de Doctorado en A´reas Estrate´gicas y Generales], Fogarty International Center-National Institutes of Health Training grant D43 TW001140, and by Intramural National Institutes of Allergy and Infectious Diseases (NIAID), National Institutes of Health. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

3

artículo

Antibody Banding Patterns of the Enzyme-Linked Immunoelectrotransfer Blot and Brain Imaging Findings in Patients with Neurocysticercosis

Publicado por
Arroyo G., Rodriguez S., Lescano A.G., Alroy K.A., Bustos J.A., Santivañez S., Pretell E.J., Garcia H.H., Gilman R.H., Gonzalez A.E., Tsang V.C.W., Gonzalez I., Saavedra H., Martinez M., Alvarado M., Verastegui M., Zimic M., Mayta H., Guerra C., Castillo Y., Castro Y., Lopez M.T., Gavidia C.M., Gomez L., Moyano L.M., Gamboa R., Muro C., Vilchez P., Nash T.E., Mahanty S., Noh J., Handali S., Friedland J.

Publicado 2018

Enlace

This work was partially supported by the Fogarty International Center/NIH (training grant numbers D43TW001140 and D43TW007393) and by the National Council for Science, Technology and Innovation of Peru (CONCYTEC).

4

artículo

Use of magnetic particles in the purification of igm antibodies against taenia solium

Publicado por
Perez L.A., Castillo Y., Espinoza C., Toribio L.M., Santos Y., Martel K.S., Wilkins P.P., Bustos J.A., García H.H., Castro-Sesquen Y.E., Gilman R.H., Gonzalez A.E., Tsang V.C.W., Rodriguez S., Gonzalez I., Saavedra H., Sanchez S., Martinez M., Verastegui M., Zimic M., Santivañez S., Mayta H., Pajuelo M., Arroyo G., Lopez M.T., Gomez L., Vargas A., Gavidia C.M., Moyano L.M., Gamboa R., Muro C., Vichez P., O´neal S., Handali S., Noh J., Nash T.E., Mahanty S., Friedland J., Grupo de Trabajo en Cisticercosis en Peru

Publicado 2020

Enlace

The use of L protein coupled magnetic particles for the concentration and purification of immunoglobulin M (mIgM) monoclonal antibodies against Taenia solium was evaluated. Three concentration methods and different elution times were evaluated and the ratio of particles to the ratio of mIgM was optimized. It is demonstrated that: 1) with the use of magnetic particles, a previous concentration of mIgM is not required, which reduces the manipulation of the antibodies and improves the recovery, 2) the use of a binding buffer can be omitted, since the pH of most cell culture supernatants are neutral, and 3) longer elution times (~ 45 minutes) are needed to increase recovery to a level greater than 80%. The study demonstrates that the use of L protein-coupled magnetic particles is a simple and efficient tool for mIgM concentration and purification. © 2020, Instituto Nacional de Salud. All ri...