Resultados de Búsqueda por Autor

1

artículo

Neurocysticercosis: unraveling the nature of the single cysticercal granuloma

Publicado por
García, H.H., Gonzalez, A.E., Rodriguez, S., Tsang, V.C.W., Pretell, E.J., Gonzales, I., Gilman, R.H.

Publicado 2010

Una sola lesión realzada en el parénquima cerebral, también llamado granuloma inflamatorio, es un diagnóstico neurológico frecuente. Una de las causas más comunes de esta lesión es la neurocisticercosis humana, la infección por las larvas de la tenia porcina, Taenia solium. Luego de la demostración de que los quistes de cisticercosis viables sobreviven en buenas condiciones durante varios años en el cerebro humano, los granulomas de cisticerco individual se han interpretado de manera sistemática como una degeneración tardía de un parásito de larga data. Sobre la base de la evidencia epidemiológica, clínica y de laboratorio detallada en este artículo, planteamos la hipótesis de que, en la mayoría de los casos, estas lesiones inflamatorias se corresponden con parásitos que mueren en los primeros pasos de la infección, probablemente como resultado natural de la inmunida...

2

artículo

Screening for cystic echinococcosis in an endemic region of Peru using portable ultrasonography and the enzyme-linked immunoelectrotransfer blot (EITB) assay

Publicado por
Moro P.L., Garcia H.H., Gonzales A.E., Bonilla J.J., Verastegui M., GilmanMD R.H.

Publicado 2005

Enlace

Financial support for this study was received from Consejo Nacional de Ciencia y Tecnología (CONCYTEC), Lima, Peru. The methods used in the present study comply with current Peruvian law governing the participation of human subjects in biomedical research.

3

revisión

Addressing post-stroke care in rural areas with Peru as a case study. Placing emphasis on evidence-based pragmatism

Publicado por
Miranda J.J., Moscoso M.G., Yan L.L., Diez-Canseco F., Málaga G., Garcia H.H., Ovbiagele B.

Publicado 2017

Enlace

Stroke is a major cause of death and disability, with most of its burden now affecting low- and middle-income countries (LMIC). People in rural areas of LMIC who have a stroke receive very little acute stroke care and local healthcare workers and family caregivers in these regions lack the necessary knowledge to assist them. Intriguingly, a recent rapid growth in cell-phone use and digital technology in rural areas has not yet been appropriately exploited for health care training and delivery purposes. What should be done in rural areas, at the community setting-level, where access to healthcare is limited remains a challenge. We review the evidence on improving post-stroke outcomes including lowering the risks of functional disability, stroke recurrence, and mortality, and propose some approaches, to target post-stroke care and rehabilitation, noting key challenges in designing suitable...

4

artículo

Changes in inflammatory gene expression in brain tissue adjacent and distant to a viable cyst in a rat model for neurocysticercosis

Publicado por
Carmen-Orozco R.P., Dávila-Villacorta D.G., Delgado-Kamiche A.D., Celiz R.H., Trompeter G., Sutherland G., Gavídia C., Garcia H.H., Gilman R.H., Verástegui M.R., Cysticercosis Working Group in Peru

Publicado 2021

Enlace

Background The parasite Taenia solium causes neurocysticercosis (NCC) in humans and is a common cause of adult-onset epilepsy in the developing world. Hippocampal atrophy, which occurs far from the cyst, is an emerging new complication of NCC. Evaluation of molecular pathways in brain regions close to and distant from the cyst could offer insight into this pathology. Methods Rats were inoculated intracranially with T. solium oncospheres. After 4 months, RNA was extracted from brain tissue samples in rats with NCC and uninfected controls, and cDNA was generated. Expression of 38 genes related to different molecular pathways involved in the inflammatory response and healing was assessed by RT-PCR array. Results Inflammatory cytokines IFN-?, TNF-?, and IL-1, together with TGF-? and ARG-1, were overexpressed in tissue close to the parasite compared to non-infected tissue. Genes for IL-1A, CS...

5

artículo

Blood–brain barrier disruption and angiogenesis in a rat model for neurocysticercosis

Publicado por
Carmen-Orozco R.P., Dávila-Villacorta D.G., Cauna Y., Bernal-Teran E.G., Bitterfeld L., Sutherland G.L., Chile N., Céliz R.H., Ferrufino-Schmidt M.C., Gavídia C.M., Sterling C.R., García H.H., Gilman R.H., Verástegui M.R.

Publicado 2019

Enlace

Neurocysticercosis (NCC) is a helminth infection affecting the central nervous system caused by the larval stage (cysticercus) of Taenia solium. Since vascular alteration and blood–brain barrier (BBB) disruption contribute to NCC pathology, it is postulated that angiogenesis could contribute to the pathology of this disease. This study used a rat model for NCC and evaluated the expression of two angiogenic factors called vascular endothelial growth factor (VEGF-A) and fibroblast growth factor (FGF2). Also, two markers for BBB disruption, the endothelial barrier antigen and immunoglobulin G, were evaluated using immunohistochemical and immunofluorescence techniques. Brain vasculature changes, BBB disruption, and overexpression of angiogenesis markers surrounding viable cysts were observed. Both VEGF-A and FGF2 were overexpressed in the tissue surrounding the cysticerci, and VEGF-A was o...

6

artículo

Antibody Banding Patterns of the Enzyme-Linked Immunoelectrotransfer Blot and Brain Imaging Findings in Patients with Neurocysticercosis

Publicado por
Arroyo G., Rodriguez S., Lescano A.G., Alroy K.A., Bustos J.A., Santivañez S., Pretell E.J., Garcia H.H., Gilman R.H., Gonzalez A.E., Tsang V.C.W., Gonzalez I., Saavedra H., Martinez M., Alvarado M., Verastegui M., Zimic M., Mayta H., Guerra C., Castillo Y., Castro Y., Lopez M.T., Gavidia C.M., Gomez L., Moyano L.M., Gamboa R., Muro C., Vilchez P., Nash T.E., Mahanty S., Noh J., Handali S., Friedland J.

Publicado 2018

Enlace

This work was partially supported by the Fogarty International Center/NIH (training grant numbers D43TW001140 and D43TW007393) and by the National Council for Science, Technology and Innovation of Peru (CONCYTEC).

7

artículo

Use of magnetic particles in the purification of igm antibodies against taenia solium

Publicado por
Perez L.A., Castillo Y., Espinoza C., Toribio L.M., Santos Y., Martel K.S., Wilkins P.P., Bustos J.A., García H.H., Castro-Sesquen Y.E., Gilman R.H., Gonzalez A.E., Tsang V.C.W., Rodriguez S., Gonzalez I., Saavedra H., Sanchez S., Martinez M., Verastegui M., Zimic M., Santivañez S., Mayta H., Pajuelo M., Arroyo G., Lopez M.T., Gomez L., Vargas A., Gavidia C.M., Moyano L.M., Gamboa R., Muro C., Vichez P., O´neal S., Handali S., Noh J., Nash T.E., Mahanty S., Friedland J., Grupo de Trabajo en Cisticercosis en Peru

Publicado 2020

Enlace

The use of L protein coupled magnetic particles for the concentration and purification of immunoglobulin M (mIgM) monoclonal antibodies against Taenia solium was evaluated. Three concentration methods and different elution times were evaluated and the ratio of particles to the ratio of mIgM was optimized. It is demonstrated that: 1) with the use of magnetic particles, a previous concentration of mIgM is not required, which reduces the manipulation of the antibodies and improves the recovery, 2) the use of a binding buffer can be omitted, since the pH of most cell culture supernatants are neutral, and 3) longer elution times (~ 45 minutes) are needed to increase recovery to a level greater than 80%. The study demonstrates that the use of L protein-coupled magnetic particles is a simple and efficient tool for mIgM concentration and purification. © 2020, Instituto Nacional de Salud. All ri...