Resultados de Búsqueda por Autor

1

artículo

Blood–brain barrier disruption and angiogenesis in a rat model for neurocysticercosis

Publicado por
Carmen-Orozco R.P., Dávila-Villacorta D.G., Cauna Y., Bernal-Teran E.G., Bitterfeld L., Sutherland G.L., Chile N., Céliz R.H., Ferrufino-Schmidt M.C., Gavídia C.M., Sterling C.R., García H.H., Gilman R.H., Verástegui M.R.

Publicado 2019

Enlace

Neurocysticercosis (NCC) is a helminth infection affecting the central nervous system caused by the larval stage (cysticercus) of Taenia solium. Since vascular alteration and blood–brain barrier (BBB) disruption contribute to NCC pathology, it is postulated that angiogenesis could contribute to the pathology of this disease. This study used a rat model for NCC and evaluated the expression of two angiogenic factors called vascular endothelial growth factor (VEGF-A) and fibroblast growth factor (FGF2). Also, two markers for BBB disruption, the endothelial barrier antigen and immunoglobulin G, were evaluated using immunohistochemical and immunofluorescence techniques. Brain vasculature changes, BBB disruption, and overexpression of angiogenesis markers surrounding viable cysts were observed. Both VEGF-A and FGF2 were overexpressed in the tissue surrounding the cysticerci, and VEGF-A was o...

2

artículo

Antibody Banding Patterns of the Enzyme-Linked Immunoelectrotransfer Blot and Brain Imaging Findings in Patients with Neurocysticercosis

Publicado por
Arroyo G., Rodriguez S., Lescano A.G., Alroy K.A., Bustos J.A., Santivañez S., Pretell E.J., Garcia H.H., Gilman R.H., Gonzalez A.E., Tsang V.C.W., Gonzalez I., Saavedra H., Martinez M., Alvarado M., Verastegui M., Zimic M., Mayta H., Guerra C., Castillo Y., Castro Y., Lopez M.T., Gavidia C.M., Gomez L., Moyano L.M., Gamboa R., Muro C., Vilchez P., Nash T.E., Mahanty S., Noh J., Handali S., Friedland J.

Publicado 2018

Enlace

This work was partially supported by the Fogarty International Center/NIH (training grant numbers D43TW001140 and D43TW007393) and by the National Council for Science, Technology and Innovation of Peru (CONCYTEC).

3

artículo

Use of magnetic particles in the purification of igm antibodies against taenia solium

Publicado por
Perez L.A., Castillo Y., Espinoza C., Toribio L.M., Santos Y., Martel K.S., Wilkins P.P., Bustos J.A., García H.H., Castro-Sesquen Y.E., Gilman R.H., Gonzalez A.E., Tsang V.C.W., Rodriguez S., Gonzalez I., Saavedra H., Sanchez S., Martinez M., Verastegui M., Zimic M., Santivañez S., Mayta H., Pajuelo M., Arroyo G., Lopez M.T., Gomez L., Vargas A., Gavidia C.M., Moyano L.M., Gamboa R., Muro C., Vichez P., O´neal S., Handali S., Noh J., Nash T.E., Mahanty S., Friedland J., Grupo de Trabajo en Cisticercosis en Peru

Publicado 2020

Enlace

The use of L protein coupled magnetic particles for the concentration and purification of immunoglobulin M (mIgM) monoclonal antibodies against Taenia solium was evaluated. Three concentration methods and different elution times were evaluated and the ratio of particles to the ratio of mIgM was optimized. It is demonstrated that: 1) with the use of magnetic particles, a previous concentration of mIgM is not required, which reduces the manipulation of the antibodies and improves the recovery, 2) the use of a binding buffer can be omitted, since the pH of most cell culture supernatants are neutral, and 3) longer elution times (~ 45 minutes) are needed to increase recovery to a level greater than 80%. The study demonstrates that the use of L protein-coupled magnetic particles is a simple and efficient tool for mIgM concentration and purification. © 2020, Instituto Nacional de Salud. All ri...