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1
artículo
Publicado 2019
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The aim of this study was to characterize the PepT1 of pirarucu Arapaima gigas through genomic and proteomic tools. A transcriptomic and proteomic analysis was made from sections of the intestine, spleen, liver and kidney of fingerlings. The transcriptomic analysis allowed obtaining two consensus sequences of 357 and 459 bp. Both fragments showed a high degree of homology (78 and 80%), mainly with nucleotide sequences coding for the PepT1 of Scleropages formosus of the same Order as A. gigas. The proteomic analysis by double mass spectrometry MALDI TOF/TOF allowed to identify 15 peptide sequences of PepT1 in pirarucu. In conclusion, PepT1 was partially characterized in the intestine of pirarucu, which could be used for further studies on the evaluation of its expression.
2
artículo
Publicado 2021
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The global spread and lethality of SARS-CoV-2 prompt the urgent need to develop an appropriate therapeutic strategy to control COVID-19 cases. The development of new drugs to fight this novel virus is urgent due to its rapid spread. Parallel alternatives have been proposed by using drugs already available for similar purposes. This review article describes theantiviral potential of ivermectin as well as its mechanisms of action against some viruses, and discusses its probable use to fight SARS-CoV-2.
3
artículo
Most Vibrio species identification methods take many days to deliver results, therefore it is important that research can be done on rapid and accurate detection and identification techniques. MALDI-TOF MS has proven to be a simple tool to identify and differentiate pathogens. The objective of this research was to recognize the available bibliographic information on the identification of Vibrio cholerae species non 01 non 139 of environmental origin by MALDI TOF MS. The Vibrio genera includes more than thirty species found in aquatic environments, some species cause diseases in both marine and human species. One of these organisms is V. cholerae, one of the most important species due to the production of cholera toxin (CT) which interrupts the transport of ions in the cells of the intestinal epithelium. The number of case reports involving V. cholerae no 01 no 139 in extraintestinal infe...
4
artículo
Publicado 2025
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The chame Dormitator latifrons is a symbolic species associated with rural aquaculture in coastal regions of the South American Pacific. Its adaptation to stay alive out of water (anhydrobiosis) for days is beneficial for its storage and transport at room temperature. The aim of the study was to determine the tolerance of chame to anhydrous conditions. Sixty chames were distributed in containers and kept in two climate-controlled environments at 16 and 24 °C for 96 h. Survival, malondialdehyde levels, total proteins and pH in the muscles and lactate dehydrogenase (LDH) activity in the gills were monitored. Additionally, the behaviour of the fish was monitored, as well as the total number of microorganisms with a sensory evaluation. The critical period of survival occurred after 48 h, where the pH decreased, and the MDA levels increased. The number of microorganisms increased in both en...
5
tesis de maestría
Publicado 2025
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La neurocisticercosis es una enfermedad parasitaria del SNC que causa epilepsia adquirida y es generada por la forma larvaria del cestodo Taenia solium. Es de curso crónico y en pacientes sintomáticos se caracteriza por convulsiones y epilepsia. Estudios en ratas de laboratorio con NCC, reportan daño axonal caracterizado por el acúmulo de proteínas (neurofilamento, APP) en forma de hinchazones axonales, que podría estar relacionado con deterioro en el transporte axonal. Objetivo: Evaluar el patrón de inmunorreactividad de las proteínas motoras kinesina y dineína como marcadores de daño en el transporte axonal en tejido cerebral de ratas Holtzman con NCC tratadas y no tratadas con antiparasitarios; y su asociación con el daño axonal. Metodología: Se utilizó tejido cerebral de ratas con NCC tratadas y no tratadas con antiparasitarios y ratas sanas sacrificadas a diferentes ti...
6
artículo
Publicado 2022
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The aim of this study was to evaluate the sedative effect of three products: valerian extract (Valeriana officinalis), benzocaine and tricaine methanesulfonate (MS-222) on stress levels produced by a simulated transport model in tilapia fingerlings. In a first part, the sedative dose of the products was determined based on the clinical characteristics that indicate depression of the central nervous system. In the second part, sedation and subsequent simulated transport for 3 hours were performed. The fish were euthanized, and blood samples were taken from the tail vein to determine cortisol (ELISA) and glucose (portable glucometer) levels, haematological parameters and mortality. The optimal doses of sedation were 200, 35 and 75 mg/l, respectively, for valerian extract, benzocaine and MS-222. After 3 h of simulated transport, cortisol and glucose values were similar or higher compared to...
7
tesis doctoral
Publicado 2025
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Universidad Nacional Agraria La Molina. Escuela de Posgrado. Doctorado en Ciencia Animal
8
artículo
Publicado 2019
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El objetivo del estudio fue caracterizar el PepT1 del paiche Arapaima gigas mediante herramientas genómicas y proteómicas. Se realizó un análisis transcriptómico y proteómico a partir de secciones del intestino, bazo, hígado y riñón de alevines. El análisis transcriptómico permitió obtener dos secuencias consenso de 357 y 459 pb. Ambos fragmentos presentaron un alto grado de homología (78 y 80%), principalmente con secuencias de nucleótidos codificantes del PepT1 de Scleropages formosus del mismo Orden que el paiche. El análisis proteómico por espectrometría de doble masa MALDI TOF/TOF permitió identificar quince secuencias peptídicas del PepT1 en paiche. Como conclusión se logró caracterizar parcialmente el PepT1 en el intestino del paiche, las cuales podrán ser usadas para posteriores estudios sobre la evaluación de su expresión.
9
artículo
Carbon dioxide (CO2), is found in nature in a gaseous state. It is a colorless, odorless, and is a key part of the carbon cycle, its concentration in the air is 0.03%. CO2 is one of the basic components in the homeostasis of human body and is a result of cellular metabolism. CO2 is 20 times more soluble in water than in air and according to many scientific studies do not leading to no clinically significant embolism. In the blood circulation the carbon dioxide is transported to the lungs by three routes: 65% as bicarbonate ion, 25% as Co2 bound to proteins, especially the hemoglobin and plasma CO2 dissolved in 10%. [1]. In European countries like France and Germany, from the Middle Ages, are known the healing properties of carbon dioxide on circulation problems and skin, and since 1920 has been applied subcutaneous CO2 for medical treatment [2]. Current studies demonstrate the effect of ...
10
tesis de grado
Publicado 2017
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La pulpa de Myrciaria Dubia (camu camu) fue atomizado en secador spray a temperaturas de operación de 195 °C y 200 °C conjugando estas temperaturas con diferentes diámetros de orificio de salida del aspersor que son de 2.5 mm y 4 mm y empleando maltodextrina como agente encapsulante en las concentraciones de 8 %, 10 % y 12 %. El diagrama de flujo elaborado para la investigación fue: materia prima Myrciaria. Dubia (camu camu), transporte, recepción/lavado, desinfección, lavado, pulpeado/refinado (malla N° 0.5 y refinado con malla N° 0.08), el polvo de camu camu fue envasado bolsas plásticas de alta densidad para después ser almacenado. Para el desarrollo experimental se siguió el modelo de análisis estadístico que empleó un diseño factorial 23 resultando la siguiente ecuación; donde la respuesta es la concentración final de ácido ascórbico (vitamina C). Vitamina C = 79...
11
tesis doctoral
Publicado 2024
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Universidad Nacional Agraria La Molina. Escuela de Posgrado. Doctorado en Ciencias e Ingeniería Biológicas
12
tesis de grado
Publicado 2025
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En este estudio se diseñó e implementó una planta piloto automatizada para la producción de leche de Tarwi, con capacidad para procesar 10 kilogramos de grano desamargado por lote. El propósito principal fue modernizar y estandarizar las etapas de molienda, cocción y pasteurización, incorporando sistemas de instrumentación y control que aseguren la calidad y la inocuidad del producto final. El sistema integra un Controlador Lógico Programable (PLC Siemens S7-1200), sensores de temperatura PT100, resistencias tipo banda para el calentamiento y un intercambiador de calor de placas para el enfriamiento. La regulación térmica se realizó mediante un algoritmo PID en lazo cerrado, lo que permitió mantener una curva estable y alcanzar una pasteurización efectiva a 75 °C durante 15 minutos, reduciendo la presencia de patógenos y conservando el contenido proteico del producto. El ...
13
tesis de grado
Publicado 1996
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This work is the development of coconut flakes Jl salty sweets using the hybrid - 121 (cocos nucifera l.) variety, its subsequent storage and transport. The samples obtained have the following characteristics: sweet pancakes; humidity 0.79%, fat 52.70%, proteins 6.10%, carbohydrates 35. 695), mineral 0.85%, fiber 3.87%; While the flakes salty presented the following composition: moisture or. HO ~ ~, fat 62.83%, proteins 6,78%, carbohydrates 22.27%, minerals 2. 30 ~ t, fiber 4. 92% reducing sugars no• presents in both samples. Was determined to do this the optimal parameters of flow of processing; control during and after the time of storage. The stages of the process involves the following operations: selection, pre-cooking, shell, mondado, washed, bleached, grated, pressed, first drying, preparation of solutions, mixing, second dried, cooled, packaged, stored and transport. In terms o...
14
artículo
Publicado 2025
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The article presents aging as a multifactorial, progressive biological process shaped by intersecting theories, with special focus on oxidative stress and free radical involvement. The Free Radical Theory of Aging posits that reactive oxygen species (ROS), produced both endogenously and via environmental exposures, induce cumulative molecular damage to lipids, proteins, and DNA impacting cellular and tissue integrity. This oxidative stress contributes to age-related degenerative diseases such as cancer and Alzheimer’s. Although antioxidants (both endogenous enzymes and exogenous dietary compounds) offer protective mechanisms against ROS, empirical evidence on their efficacy in extending lifespan remains inconclusive, largely due to bioavailability challenges and systemic complexity. The article also addresses mitochondrial dysfunction and genomic instability especially mitochondrial DN...
15
artículo
Publicado 2025
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The article presents aging as a multifactorial, progressive biological process shaped by intersecting theories, with special focus on oxidative stress and free radical involvement. The Free Radical Theory of Aging posits that reactive oxygen species (ROS), produced both endogenously and via environmental exposures, induce cumulative molecular damage to lipids, proteins, and DNA impacting cellular and tissue integrity. This oxidative stress contributes to age-related degenerative diseases such as cancer and Alzheimer’s. Although antioxidants (both endogenous enzymes and exogenous dietary compounds) offer protective mechanisms against ROS, empirical evidence on their efficacy in extending lifespan remains inconclusive, largely due to bioavailability challenges and systemic complexity. The article also addresses mitochondrial dysfunction and genomic instability especially mitochondrial DN...
16
artículo
Publicado 2019
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Se analizaron las características de una cepa de Bacillus amyloliquefaciens aislada del intestino de Arapaima gigas, seleccionada por su capacidad para generar inhibición de múltiples bacterias patógenas de peces, mediante el uso de herramientas moleculares como PCR y espectrometría de masas - MALDI TOF/TOF. Los resultados mostraron a través de PCR que esta cepa cuenta con genes claves para la generación de péptidos antimicrobianos como bmyB (bacillomycin L synthetase B), fenD (fengycin sintetasa), srfAA (subunidad 1 surfactin sintetasa), bacA (proteína de biosíntesis de bacilysin) e iturin (iturin A). Además, mediante el análisis por espectrometría de masas se detectaron bacteriocinas (plipastatin, gramicidin, fengycin, surfactin), proteínas de fijación al intestino (like-enolase), proteinas transportadoras de péptidos antimicrobianos (ABC-transporters) y proteínas de e...
17
artículo
Publicado 2019
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The characteristics of a strain of Bacillus amyloliquefaciens isolated from the intestine of Arapaima gigas, selected for its ability to generate inhibition of multiple pathogenic bacteria of fishes were analyzed by using molecular tools such as PCR and mass spectrometry - MALDI TOF / TOFM. The results showed through PCR that this strain has key genes for the generation of antimicrobial peptides such as bmyB (bacillomycin L synthetase B), fenD (fengycin synthetase), srfAA (subunit 1 surfactin synthetase), bacA (bacilysin biosynthesis protein) and iturin (iturin A). In addition, by mass spectrometry analysis were detected bacteriocins (plipastatin, gramicidin, fengycin, surfactin), intestine binding proteins (like-enolase), antimicrobial peptide transport proteins (ABC-transporters) and immune system stimulation proteins like flagelin. Collagenase, chitinases and xylose-isomerases protein...
18
artículo
Publicado 2023
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The empirical use of alpaca rennet in the production of artisanal cheeses with cow’s milk is widespread in the high Andean communities of Perú and Bolivia, and has not been scientifically reported. The objective of the present study was to characterize alpaca rennet and evaluate its coagulating activity. Ready-to-use alpaca curds were collected directly from dairies in four neighborhoods of Viscapalca (Huancavelica-Perú, 4003 masl), which were transported under refrigeration and kept frozen at -20°C. The Crude Enzymatic Extract (CEE) was obtained by centrifugation at 560 x g for 10 min at 4°C, from which proteins were determined by Bradford, SDS-PAGE electrophoretic profile, coagulation time and proteolytic activity. The traditional management of alpaca rennet is also described. CEE was found to contain 29.80 to 52.91 µg/ml of protein. The SDS-PAGE electrophoretic profile revealed...
19
artículo
Publicado 2023
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The empirical use of alpaca rennet in the production of artisanal cheeses with cow’s milk is widespread in the high Andean communities of Perú and Bolivia, and has not been scientifically reported. The objective of the present study was to characterize alpaca rennet and evaluate its coagulating activity. Ready-to-use alpaca curds were collected directly from dairies in four neighborhoods of Viscapalca (Huancavelica-Perú, 4003 masl), which were transported under refrigeration and kept frozen at -20°C. The Crude Enzymatic Extract (CEE) was obtained by centrifugation at 560 x g for 10 min at 4°C, from which proteins were determined by Bradford, SDS-PAGE electrophoretic profile, coagulation time and proteolytic activity. The traditional management of alpaca rennet is also described. CEE was found to contain 29.80 to 52.91 µg/ml of protein. The SDS-PAGE electrophoretic profile revealed...
20
Publicado 2022
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El objetivo de este trabajo de investigación fue la digestión de proteínas inicia con la ingesta de alimentos por medio de la boca en el que se mezcla con la segregación de saliva de las glándulas salivales, recorre el esófago para posteriormente ser degradas por las enzimas del estómago que desnaturaliza las proteínas, continuando con el recorrido en el intestino delgado por acción de las enzimas intestinales y enzimas pancreáticas generan un producto final de aminoácidos libres, dipéptidos y tripéptidos, iniciando la absorción de aminoácidos. En primera instancia los aminoácidos libres son absorbidos por los transportadores secundario de los enterocitos mediante la difusión facilitada y llevada a la sangre, además este proceso es impulsado por la bomba de sodio y potasio (ciclo de Krebs). Los péptidos y tripéptidos ingresan por un transportador, en esa zona del ente...
