The aim of this study was to characterize the PepT1 of pirarucu Arapaima gigas through genomic and proteomic tools. A transcriptomic and proteomic analysis was made from sections of the intestine, spleen, liver and kidney of fingerlings. The transcriptomic analysis allowed obtaining two consensus sequences of 357 and 459 bp. Both fragments showed a high degree of homology (78 and 80%), mainly with nucleotide sequences coding for the PepT1 of Scleropages formosus of the same Order as A. gigas. The proteomic analysis by double mass spectrometry MALDI TOF/TOF allowed to identify 15 peptide sequences of PepT1 in pirarucu. In conclusion, PepT1 was partially characterized in the intestine of pirarucu, which could be used for further studies on the evaluation of its expression.

El objetivo del estudio fue caracterizar el PepT1 del paiche Arapaima gigas mediante herramientas genómicas y proteómicas. Se realizó un análisis transcriptómico y proteómico a partir de secciones del intestino, bazo, hígado y riñón de alevines. El análisis transcriptómico permitió obtener dos secuencias consenso de 357 y 459 pb. Ambos fragmentos presentaron un alto grado de homología (78 y 80%), principalmente con secuencias de nucleótidos codificantes del PepT1 de Scleropages formosus del mismo Orden que el paiche. El análisis proteómico por espectrometría de doble masa MALDI TOF/TOF permitió identificar quince secuencias peptídicas del PepT1 en paiche. Como conclusión se logró caracterizar parcialmente el PepT1 en el intestino del paiche, las cuales podrán ser usadas para posteriores estudios sobre la evaluación de su expresión.

Tilapia Lake Virus (TiLV) is a pathogen that causes massive mortalities in both cultured and wild tilapia populations around the world. The development of an effective vaccine against this emerging pathogen is imperative to prevent economic losses. In this work an expression vector was designed and evaluated as a potential DNA vaccine against this virus. Initially, a threading analysis was done to predict the three-dimensional structures and functions of the TiLV proteins. Structural homologies were found between the TiLV proteins corresponding to the genomic segment 1 and the genomic segment 4, with the RNA-dependent RNA polymerase proteins of the influenza B virus (56%) and the neuraminidase protein belonging to the influenza A virus capsid (12%), respectively. The PCR product of the viral neuraminidase gene was inserted into the expression plasmid vector pCMV. Finally, the plasmid con...

Tilapia Lake Virus (TiLV) is a pathogen that causes massive mortalities in both cultured and wild tilapia populations around the world. The development of an effective vaccine against this emerging pathogen is imperative to prevent economic losses. In this work an expression vector was designed and evaluated as a potential DNA vaccine against this virus. Initially, a threading analysis was done to predict the three-dimensional structures and functions of the TiLV proteins. Structural homologies were found between the TiLV proteins corresponding to the genomic segment 1 and the genomic segment 4, with the RNA-dependent RNA polymerase proteins of the influenza B virus (56%) and the neuraminidase protein belonging to the influenza A virus capsid (12%), respectively. The PCR product of the viral neuraminidase gene was inserted into the expression plasmid vector pCMV. Finally, the plasmid con...

Como consecuencia de las grandes pérdidas económicas ocasionadas en el sector camaronero por el brote de enfermedades, la producción de lotes de camarones SPR ha empezado a tomar auge. Es por ello que varios trabajos de investigación han identificado diferentes efectores relacionados con la respuesta inmune celular y humoral, incluyendo el sistema ARNi, a fin de establecer marcadores moleculares aplicables dentro de los programas GAS. El estudio permitió determinar el patrón de expresión de dos de los principales genes (Dicer-2 y Argonaute-2) relacionados con el sistema ARNi como respuesta inmune antiviral en Litopenaeus vannamei. A partir de aplicación de dos inmunoestimulantes (LPS + Poly I:C) los niveles de expresión del gen Dicer-2 se incrementaron gradualmente durante las primeras 09H00 post-inducción, entre 6.5 (03H00), 8.8 (06H00) y 10.7 (09H00) veces mayores en comparac...

Como consecuencia de las grandes pérdidas económicas ocasionadas en el sector camaronero por el brote de enfermedades, la producción de lotes de camarones SPR ha empezado a tomar auge. Es por ello que varios trabajos de investigación han identificado diferentes efectores relacionados con la respuesta inmune celular y humoral, incluyendo el sistema ARNi, a fin de establecer marcadores moleculares aplicables dentro de los programas GAS. El estudio permitió determinar el patrón de expresión de dos de los principales genes (Dicer-2 y Argonaute-2) relacionados con el sistema ARNi como respuesta inmune antiviral en Litopenaeus vannamei. A partir de aplicación de dos inmunoestimulantes (LPS + Poly I:C) los niveles de expresión del gen Dicer-2 se incrementaron gradualmente durante las primeras 09H00 post-inducción, entre 6.5 (03H00), 8.8 (06H00) y 10.7 (09H00) veces mayores en comparac...

The aim of this study was to characterize the microbiota of the intestinal tract of robalo (Centropomus sp), from natural environment and captivity, and to isolate and identify bacteria with probiotic potential. The intestinal microbiota was characterized by metagenomics directed to the 16S rRNA gene. In addition, samples of intestinal mucus were cultured in MRS medium for bacterial isolation. Molecular identification was done by sequencing the 16S rRNA gene. Probiotic capabilities were evidenced by proteolytic and bacterial antagonism assays against Plesiomona shigelloides, Aeromonas hydrophila, Aeromonas veronii and Vibrio harveyi. The most abundant bacterial genera were Clostridium, Photobacterium, Cetobacterium and Rubritepida. Bacterial species, including Klebsiella sp, two strains of Weisiella cibaria and Lactococcus sp were isolated on MRS agar from the intestinal tract. W. cibari...

The aim of this study was to characterize the microbiota of the intestinal tract of robalo (Centropomus sp), from natural environment and captivity, and to isolate and identify bacteria with probiotic potential. The intestinal microbiota was characterized by metagenomics directed to the 16S rRNA gene. In addition, samples of intestinal mucus were cultured in MRS medium for bacterial isolation. Molecular identification was done by sequencing the 16S rRNA gene. Probiotic capabilities were evidenced by proteolytic and bacterial antagonism assays against Plesiomona shigelloides, Aeromonas hydrophila, Aeromonas veronii and Vibrio harveyi. The most abundant bacterial genera were Clostridium, Photobacterium, Cetobacterium and Rubritepida. Bacterial species, including Klebsiella sp, two strains of Weisiella cibaria and Lactococcus sp were isolated on MRS agar from the intestinal tract. W. cibari...