The production of tilapia (Oreochromis sp) is being affected by outbreaks of lake tilapia virus (TiLV) with high mortality in the main breeding regions in Peru. The aim of this study was to determine the presence of TiLV in two tilapia fish farms in Piura and San Martín, which presented mortalities greater than 60%. Samples (liver and brain) were taken from 70 fish from different stages of culture that showed lethargy, loss of appetite, eye lesions and skin erosions. The detection and confirmation of TiLV was determined by semi-nested RT-PCR and subsequent sequencing of the amplified product of segment 3 of the virus. Products of the expected weight (250 bp) corresponding to samples from the two production centres were found. The phylogenetic analysis showed a high homology among the isolates and an identity greater than 90% with the Israeli reference strain (KU751816.1).

The production of tilapia (Oreochromis sp) is being affected by outbreaks of lake tilapia virus (TiLV) with high mortality in the main breeding regions in Peru. The aim of this study was to determine the presence of TiLV in two tilapia fish farms in Piura and San Martín, which presented mortalities greater than 60%. Samples (liver and brain) were taken from 70 fish from different stages of culture that showed lethargy, loss of appetite, eye lesions and skin erosions. The detection and confirmation of TiLV was determined by semi-nested RT-PCR and subsequent sequencing of the amplified product of segment 3 of the virus. Products of the expected weight (250 bp) corresponding to samples from the two production centres were found. The phylogenetic analysis showed a high homology among the isolates and an identity greater than 90% with the Israeli reference strain (KU751816.1).

La tilapia es la segunda especie acuícola más importante en la acuicultura mundial. En los últimos años, este cultivo se ha visto amenazado por la aparición de un nuevo patógeno viral conocido como virus de la tilapia del lago (TiLV), que causa altas tasas de mortalidad tanto a tilapias silvestres como de cultivo. El presente estudio reportó la identificación del TiLV en diferentes provincias del Perú, tanto de medios naturales como piscigranjas comerciales. Los peces enfermos exhibieron letargia, inapetencia, lesiones oculares, erosiones en la piel y tasas de mortalidad mayores al 60%. La presencia de TiLV fue confirmada en 14 (51,85%) de las 27 áreas muestreadas mediante seminested RT-PCR y confirmadas posteriormente mediante secuenciamiento del producto amplificado perteneciente al segmento 3. Los resultados revelaron una alta homología entre aislados del Perú (Piura, San ...

En especies de alta producción como la tilapia y trucha, la presencia de enfermedades infecciosas puede impactar significativamente la acuicultura. La escasez de datos sobre frecuencias de patógenos en los lugares de crianza crea un vacío de información que podría limitar respuestas oportunas ante futuros brotes. El objetivo del estudio fue determinar la frecuencia de agentes bacterianos y virales en trucha, tilapia y paiche cultivados, a partir de informes de un laboratorio privado de Piura, durante el periodo 2020-2023. Se registraron datos de los informes de muestras procesadas (especie, estadio, procedencia, estacionalidad, tipo de notificación) y el resultado de diagnóstico (agente etiológico). En total se realizaron 1348 pruebas moleculares, de las cuales 80 resultaron positivas. De estas, el 58.8% (47/80) correspondieron a etiología bacteriana y el 41.3% (33/80) a etiolog...

The aim of this study was to characterize the microbiota of the intestinal tract of robalo (Centropomus sp), from natural environment and captivity, and to isolate and identify bacteria with probiotic potential. The intestinal microbiota was characterized by metagenomics directed to the 16S rRNA gene. In addition, samples of intestinal mucus were cultured in MRS medium for bacterial isolation. Molecular identification was done by sequencing the 16S rRNA gene. Probiotic capabilities were evidenced by proteolytic and bacterial antagonism assays against Plesiomona shigelloides, Aeromonas hydrophila, Aeromonas veronii and Vibrio harveyi. The most abundant bacterial genera were Clostridium, Photobacterium, Cetobacterium and Rubritepida. Bacterial species, including Klebsiella sp, two strains of Weisiella cibaria and Lactococcus sp were isolated on MRS agar from the intestinal tract. W. cibari...

The aim of this study was to characterize the microbiota of the intestinal tract of robalo (Centropomus sp), from natural environment and captivity, and to isolate and identify bacteria with probiotic potential. The intestinal microbiota was characterized by metagenomics directed to the 16S rRNA gene. In addition, samples of intestinal mucus were cultured in MRS medium for bacterial isolation. Molecular identification was done by sequencing the 16S rRNA gene. Probiotic capabilities were evidenced by proteolytic and bacterial antagonism assays against Plesiomona shigelloides, Aeromonas hydrophila, Aeromonas veronii and Vibrio harveyi. The most abundant bacterial genera were Clostridium, Photobacterium, Cetobacterium and Rubritepida. Bacterial species, including Klebsiella sp, two strains of Weisiella cibaria and Lactococcus sp were isolated on MRS agar from the intestinal tract. W. cibari...