The aim of this study was to characterize the microbiota of the intestinal tract of robalo (Centropomus sp), from natural environment and captivity, and to isolate and identify bacteria with probiotic potential. The intestinal microbiota was characterized by metagenomics directed to the 16S rRNA gene. In addition, samples of intestinal mucus were cultured in MRS medium for bacterial isolation. Molecular identification was done by sequencing the 16S rRNA gene. Probiotic capabilities were evidenced by proteolytic and bacterial antagonism assays against Plesiomona shigelloides, Aeromonas hydrophila, Aeromonas veronii and Vibrio harveyi. The most abundant bacterial genera were Clostridium, Photobacterium, Cetobacterium and Rubritepida. Bacterial species, including Klebsiella sp, two strains of Weisiella cibaria and Lactococcus sp were isolated on MRS agar from the intestinal tract. W. cibari...

The aim of this study was to characterize the microbiota of the intestinal tract of robalo (Centropomus sp), from natural environment and captivity, and to isolate and identify bacteria with probiotic potential. The intestinal microbiota was characterized by metagenomics directed to the 16S rRNA gene. In addition, samples of intestinal mucus were cultured in MRS medium for bacterial isolation. Molecular identification was done by sequencing the 16S rRNA gene. Probiotic capabilities were evidenced by proteolytic and bacterial antagonism assays against Plesiomona shigelloides, Aeromonas hydrophila, Aeromonas veronii and Vibrio harveyi. The most abundant bacterial genera were Clostridium, Photobacterium, Cetobacterium and Rubritepida. Bacterial species, including Klebsiella sp, two strains of Weisiella cibaria and Lactococcus sp were isolated on MRS agar from the intestinal tract. W. cibari...

Un evento de mortalidad natural de tilapia nilótica en la laguna El Sauce, región San Martin, Perú, fue observado en el mes de noviembre del 2018. Una cepa bacteriana (L3-1 PB) fue aislada a partir de las lesiones en la piel de dos ejemplares de tilapia. Basados en la secuenciación del gen 16S ADNr y análisis filogenético, el aislado fue identificado como Lactococcus garvieae, y confirmado mediante la reacción en cadena de la polimerasa PCR utilizando cebadores específicos. Para demostrar la patogenicidad de la cepa, se realizó una infección experimental mediante inyección intraperitoneal con la bacteria (4.8106 UFC/ml), causando una mortalidad acumulada del 50 %. Por otro lado, mediante la técnica de espectrometría de doble masa MALDI TOF-TOF se identificó secuencias de aminoácidos correspondientes a diversos factores de virulencia. Estos resultados sugieren que L. garvie...

El Objetivo general del presente trabajo de suficiencia profesional es el diagnosticar mediante técnicas moleculares las enfermedades virales y bacterianas en el cultivo de tilapia en la empresa Pezbiotec SAC.