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Pacientes diagnosticados con Fibrosis Quística provenientes del Instituto de Salud del Niño del Ministerio de Salud del Perú y del Hospital IV E. Rebagliatti Martins (Seguridad Social), fueron evaluados durante el segundó semestre del 2013 con la finalidad de detectar mutaciones del Gen C.F.T.R. (Regulador de la Conductancia Transmembrana de la Fibrosis Quística). Mediante el método de la A.R.M.S-P.C.R (Reacción en Cadena de la Polimerasa- Sistema de amplificación de la Mutación Refractaria), se analizaron 28 muestras, encontrándose que en el 21,42% estaban presente dos mutaciones y el 32,21% una mutación. La prevalencia de DF508 en Perú, es relativamente baja (31,30%) en comparación con el promedio latinoamericano (43,54%). Al contrario los pacientes peruanos presentan una tasa importante de G542X (7,40%) en comparación con los de Latinoamérica (5,10%).

Pacientes diagnosticados con Fibrosis Quística provenientes del Instituto de Salud del Niño del Ministerio de Salud del Perú y del Hospital IV E. Rebagliatti Martins (Seguridad Social), fueron evaluados durante el segundó semestre del 2013 con la finalidad de detectar mutaciones del Gen C.F.T.R. (Regulador de la Conductancia Transmembrana de la Fibrosis Quística). Mediante el método de la A.R.M.S-P.C.R (Reacción en Cadena de la Polimerasa- Sistema de amplificación de la Mutación Refractaria), se analizaron 28 muestras, encontrándose que en el 21,42% estaban presente dos mutaciones y el 32,21% una mutación. La prevalencia de DF508 en Perú, es relativamente baja (31,30%) en comparación con el promedio latinoamericano (43,54%). Al contrario los pacientes peruanos presentan una tasa importante de G542X (7,40%) en comparación con los de Latinoamérica (5,10%).

Tilapia Lake Virus (TiLV) is a pathogen that causes massive mortalities in both cultured and wild tilapia populations around the world. The development of an effective vaccine against this emerging pathogen is imperative to prevent economic losses. In this work an expression vector was designed and evaluated as a potential DNA vaccine against this virus. Initially, a threading analysis was done to predict the three-dimensional structures and functions of the TiLV proteins. Structural homologies were found between the TiLV proteins corresponding to the genomic segment 1 and the genomic segment 4, with the RNA-dependent RNA polymerase proteins of the influenza B virus (56%) and the neuraminidase protein belonging to the influenza A virus capsid (12%), respectively. The PCR product of the viral neuraminidase gene was inserted into the expression plasmid vector pCMV. Finally, the plasmid con...

Tilapia Lake Virus (TiLV) is a pathogen that causes massive mortalities in both cultured and wild tilapia populations around the world. The development of an effective vaccine against this emerging pathogen is imperative to prevent economic losses. In this work an expression vector was designed and evaluated as a potential DNA vaccine against this virus. Initially, a threading analysis was done to predict the three-dimensional structures and functions of the TiLV proteins. Structural homologies were found between the TiLV proteins corresponding to the genomic segment 1 and the genomic segment 4, with the RNA-dependent RNA polymerase proteins of the influenza B virus (56%) and the neuraminidase protein belonging to the influenza A virus capsid (12%), respectively. The PCR product of the viral neuraminidase gene was inserted into the expression plasmid vector pCMV. Finally, the plasmid con...

Estudio para determinar la frecuencia de las diez mutaciones más comúnmente reportadas en América Latina del gen CFTR mediante Sistema de Mutación Refractario a la amplificación por PCR (ARMS-PCR) en los pacientes con fibrosis quística (FQ) del Hospital Nacional Edgardo Rebagliati Martins y el Instituto Nacional de Salud del Niño, en Lima, Perú, durante el año 2014.

Antimicrobial peptides are small molecules that exhibit strong antagonistic activity against pathogenic microorganisms and are synthesized by a wide variety or organisms, including probiotic bacteria. The aim of the study was to identify peptides with antimicrobial activity in the Enterococcus faecium IP5-2a strain previously isolated from the intestinal tract of Nile tilapia (Oreochromis niloticus). Cellular and extracelullar proteins extraction was performed, followed by 1D SDS-PAGE gel electrophoresis and MALDI-TOF/TOF mass spectrometry. The results revealed peptides with antimicrobial activity, including P54 (SagA) protein, a bacteriocin-like protein and a lantibiotic. These proteins play an important role in the antagonistic capacity of the E. faecium IP52a strain, allowing it to be considered as a potential probiotic in the aquaculture industry.