Antimicrobial peptides are small molecules that exhibit strong antagonistic activity against pathogenic microorganisms and are synthesized by a wide variety or organisms, including probiotic bacteria. The aim of the study was to identify peptides with antimicrobial activity in the Enterococcus faecium IP5-2a strain previously isolated from the intestinal tract of Nile tilapia (Oreochromis niloticus). Cellular and extracelullar proteins extraction was performed, followed by 1D SDS-PAGE gel electrophoresis and MALDI-TOF/TOF mass spectrometry. The results revealed peptides with antimicrobial activity, including P54 (SagA) protein, a bacteriocin-like protein and a lantibiotic. These proteins play an important role in the antagonistic capacity of the E. faecium IP52a strain, allowing it to be considered as a potential probiotic in the aquaculture industry.

Probiotic bacteria in aquaculture represent a control alternative and prevention of pathogens, in addition, reduce the use of antibiotics. However, it is necessary to search for other groups of bacteria with similar or better properties, obtained even from the same source. The aim of the study was to identify molecularly lactic acid bacteria with probiotic properties isolated from the posterior intestine of Nile tilapia (Oreochromis niloticus). The study was carried out from November 2017 to March 2018 evaluating the antagonistic, proteolytic, amylolytic, and antibiotic sensitivity activities, and antagonistic to the following pathogens: Aeromonas hydrophila, Aeromonas veronii, Pseudomonas aeruginosa, Pseudomonas putida and Plesiomonas shigelloides. It was identified by sequencing the 16S rRNA gene that Enterococcus faecium (IP5-2a) and Pedioc...

Probiotic bacteria in aquaculture represent a control alternative and prevention of pathogens, in addition, reduce the use of antibiotics. However, it is necessary to search for other groups of bacteria with similar or better properties, obtained even from the same source. The aim of the study was to identify molecularly lactic acid bacteria with probiotic properties isolated from the posterior intestine of Nile tilapia (Oreochromis niloticus). The study was carried out from November 2017 to March 2018 evaluating the antagonistic, proteolytic, amylolytic, and antibiotic sensitivity activities, and antagonistic to the following pathogens: Aeromonas hydrophila, Aeromonas veronii, Pseudomonas aeruginosa, Pseudomonas putida and Plesiomonas shigelloides. It was identified by sequencing the 16S rRNA gene that Enterococcus faecium (IP5-2a) and Pedioc...

Probiotic bacteria in aquaculture represent a control alternative and prevention of pathogens, in addition, reduce the use of antibiotics. However, it is necessary to search for other groups of bacteria with similar or better properties, obtained even from the same source. The aim of the study was to identify molecularly lactic acid bacteria with probiotic properties isolated from the posterior intestine of Nile tilapia (Oreochromis niloticus). The study was carried out from November 2017 to March 2018 evaluating the antagonistic, proteolytic, amylolytic, and antibiotic sensitivity activities, and antagonistic to the following pathogens: Aeromonas hydrophila, Aeromonas veronii, Pseudomonas aeruginosa, Pseudomonas putida and Plesiomonas shigelloides. It was identified by sequencing the 16S rRNA gene that Enterococcus faecium (IP5-2a) and Pedioc...

Probiotic bacteria in aquaculture represent a control alternative and prevention of pathogens, in addition, reduce the use of antibiotics. However, it is necessary to search for other groups of bacteria with similar or better properties, obtained even from the same source. The aim of the study was to identify molecularly lactic acid bacteria with probiotic properties isolated from the posterior intestine of Nile tilapia (Oreochromis niloticus). The study was carried out from November 2017 to March 2018 evaluating the antagonistic, proteolytic, amylolytic, and antibiotic sensitivity activities, and antagonistic to the following pathogens: Aeromonas hydrophila, Aeromonas veronii, Pseudomonas aeruginosa, Pseudomonas putida and Plesiomonas shigelloides. It was identified by sequencing the 16S rRNA gene that Enterococcus faecium (IP5-2a) and Pedioc...