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1
tesis de grado
Publicado 2020
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El informe de tesis titulado: “APLICACIÓN DE PROGRAMAS DE TALLERES “SHUTIKUNAWAN PUKLLANA” PARA MEJORAR EL NIVEL DE DESARROLLO DE LA COMPETENCIA DEL ÁREA DE COMUNICACIÓN - PRODUCE TEXTOS ESCRITOS – EN NIÑOS DE 4 AÑOS DE LA INSTITUCIÓN EDUCATIVA PARTICULAR JESÚS MAESTRO. NUEVO CHIMBOTE 2018”, tiene la finalidad de determinar el nivel de influencia la aplicación de programas de talleres, debido que durante el trabajo a diario en el aula no se presenta adecuadamente actividades, que ayude a mejorar, esto se debe por la carencia de información con respecto a cómo los niños aprenden a través del juego para su iniciación a la producción de textos escritos. La investigación se caracterizó por un tipo de estudio aplicada con diseño cuasi experimental, donde se empleó a un total poblacional compuesto por 2 aulas, posteriormente se seleccionó el grupo control y el expe...
2
tesis de maestría
Publicado 2018
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El trabajo académico, en su contenido tiene los siguientes acápites: En el primer acápite, la formulación del problema identificado, en el que se describe las características del contexto utilizando el árbol de problemas se describe las causas y se determina los efectos de este en la formulación del problema. Luego se contextualiza el problema con el enunciado: Nivel insatisfactorio de aprendizaje en la competencia produce textos escritos, del área de comunicación, en los estudiantes del IV Ciclo de Educación Básica Regular de la IE Nº 81503 de Santa Clara del distrito de Casa Grande, Ugel Ascope. En el segundo acápite, la vinculación del problema con los procesos de la institución educativa, los compromisos de gestión escolar y el Marco del Buen Desempeño del director, existe una relación muy ceñida al problema priorizado en el centro educativo. En el acápite tercero...
3
artículo
Publicado 2016
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Surfactants are amphipathic molecules which reduce stress at the interface, thereby increasing water solubility and availability of organic compounds are produced by bacteria, fungi, and yeasts. For the isolation of biosurfactant producing bacteria and lipases, was plant in inducing means 10% of sewage effluent from slaughterhouses and soils contaminated with hydrocarbons Province taps Trujillo - Peru. Isolates were seed in agar cultures lecithin and rhodamine agar for determination of lipase producers. The selected microorganisms were plate on blood agar and agar Siegmund and Wagner (SW) for preliminary selection of biosurfactants bacteria. Later it determined to those microorganisms producing surfactants by Emulsification Index (EI) and measurement of surface tension. Positive cultures these tests were select and identified by API galleries and molecular identification by DNA sequencin...
4
artículo
Publicado 2016
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Surfactants are amphipathic molecules which reduce stress at the interface, thereby increasing water solubility and availability of organic compounds are produced by bacteria, fungi, and yeasts. For the isolation of biosurfactant producing bacteria and lipases, was plant in inducing means 10% of sewage effluent from slaughterhouses and soils contaminated with hydrocarbons Province taps Trujillo - Peru. Isolates were seed in agar cultures lecithin and rhodamine agar for determination of lipase producers. The selected microorganisms were plate on blood agar and agar Siegmund and Wagner (SW) for preliminary selection of biosurfactants bacteria. Later it determined to those microorganisms producing surfactants by Emulsification Index (EI) and measurement of surface tension. Positive cultures these tests were select and identified by API galleries and molecular identification by DNA sequencin...
5
artículo
Publicado 2015
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Background: Resistance by extended-spectrum beta-lactamase (ESBL) is the most common antimicrobial resistance and of public health importance. Objectives: To describe ESBL producing bacteria characteristics in a national reference hospital. Design: Cross sectional, descriptive study. Setting: Hospital Nacional Daniel Alcides Carrión, Callao, Peru. Materials: Records of secretion cultures done in the hospital Laboratory of Microbiology during 2012. Methods: Patient data (age, sex and service from which the sample was received) and sample data (date of collection, sample type, microorganism found, sensitivity and detailed classification as ESBLproducing bacteria) were analyzed. Main outcome measures: Characteristics of infections by ESBL-producing bacterias. Results: Study included 3 149 samples, 70.9% (2 235) from female patients; 29.4% were cultures positive for ESBL-producing bacteria....
6
artículo
Publicado 2020
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Objetive. Communicate the first report of Klebsiella pneumoniae producing carbapenemases. The study: Microbiological diagnosis using phenotypic methods: double disc synergy, modified Hodge test, immunochromatography and carbapenemic inactivation. Findings: Four isolates (urine 3 and blood 1) of carbapenemase-producing K. pneumoniae were confirmed, which also presented a MIC of 4 and> = 8 for ertapenem and> = 16 µg / mL at imipenem and meropenem. Conclusion: Standardized phenotypic methods are useful in the confirmation of carbapenemases and with it an alternative for the best taking of therapeutic options and epidemiological surveillance.
7
artículo
Publicado 2018
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Objective: To determine the clonality pattern assessed by ERIC-PCR and REP-PCR in extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae isolates from patients with urinary tract infections at the Hospital Regional Lambayeque (HRL) from July to November 2015. Materials and methods: A total of 30 ESBL-producing E. coli and K. pneumoniae clinical isolates collected from the HRL’s emergency, medicine, surgery and pediatrics services were assessed. The clonal relationship was determined using the ERIC-PCR and REP-PCR markers. For clusters, UPGMA algorithm with Bio Rad Quantity One 1-D analysis software was used, thus generating dendrograms through the union of the electrophoretic profiles obtained by both molecular markers. Results: From the molecular analysis, three predominant clonal patterns were found in E. coli and two in K. pneumoniae. Conclusion...
8
artículo
Publicado 2024
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The valorization and use of lignocellulosic biomass from food processing to obtain value-added products is crucial to improve sustainability and reduce waste management costs. This potential for cost reduction is a reason for optimism, as it allows agro-industrial waste to be transformed into valuable resources, contributing to the circular economy. This study focused on the compositional and structural characterization of eleven types of lignocellulosic biomass (LCB) to evaluate their potential in producing nanopolysaccharides and polyhydroxyalkanoates. Parameters such as humidity, ash, proteins, extractives, structural carbohydrates, and lignin were analyzed in passion fruit peels, artichoke bracts, asparagus peels, lemon peels, orange peels, avocado seed, potato peels, cassava peels, sugar cane bagasse, rice husk, and rice straw. The results showed that fruit peels and other waste had...
9
artículo
Publicado 2012
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The aim of this study was to characterize halotolerant bacteria producing hydrolases of biotechnological interest isolated from hot springs from Tarapoto. For that, water samples were collected and plated on trypticase soy agar containing 5% sodium chloride at 37 °C for 24 h. 14 aerobic bacterial isolates were selected to show colonies of different size, colour and consistency, then morphological and physiological properties and hydrolytic capacity to tween 80, starch, gelatin, carboximetilcellulose, lactose were determined. The 14 isolates were grouped according to substrate hydrolyzation profiles in groups: I (starch), II (lactose), III (Tween 80 and gelatin) , IV (tween 80 and starch ), V (starch, gelatin, lactose), VI (tween 80, starch and gelatin) corresponding in number of bacteria 1; 1; 1; 5; 1,5; respectively. 86% (12/14) of isolates hydrolyzed more than one substrate, but none ...
10
artículo
Publicado 2012
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The aim of this study was to characterize halotolerant bacteria producing hydrolases of biotechnological interest isolated from hot springs from Tarapoto. For that, water samples were collected and plated on trypticase soy agar containing 5% sodium chloride at 37 °C for 24 h. 14 aerobic bacterial isolates were selected to show colonies of different size, colour and consistency, then morphological and physiological properties and hydrolytic capacity to tween 80, starch, gelatin, carboximetilcellulose, lactose were determined. The 14 isolates were grouped according to substrate hydrolyzation profiles in groups: I (starch), II (lactose), III (Tween 80 and gelatin) , IV (tween 80 and starch ), V (starch, gelatin, lactose), VI (tween 80, starch and gelatin) corresponding in number of bacteria 1; 1; 1; 5; 1,5; respectively. 86% (12/14) of isolates hydrolyzed more than one substrate, but none ...
11
artículo
Publicado 2020
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Objective: To determine the frequency of extended spectrum β-lactamases (ESBL) in clinical infections caused by Escherichia coli y Klebsiella pneumoniae and the frequency of CTX-M among them at the Instituto Nacional de Salud del Niño-Breña, Lima, Peru. Methods: ESBL producing strains of E. coli and K. pneumoniae collected from August 2012 and January 2013 were analyzed; a total of 724 E. coli and 181 K. pneumoniae consecutive, non- repeated isolates from community and hospital acquired infections were included. Identification was performed by conventional biochemistry, ESBL phenotype was detected following the Jarlier´s method and PCR was used to detect CTX-M. Results: overall prevalence of ESBL was 31% (281 strains); 207/724 (28.6%) E. coli and 74/181 (40.9%) K. pneumoniae. The bla gene was detected in 256 of ESBL producing strains (91.1%). Conclusions: The CTX-M phe...
12
artículo
The dosage Chorionic Gonadotropin, following the technique of Mainini Galli, is a simple and quick laboratory test, others whose clinical significance is emphasized. To perform the dosoie Chorionic Gonadotropin, following the above technique, it is necessary to know the minimum dose capable of producing positive biological response in the male toad. 1 The values given in this figure, the different authors vary, and this variation is due to: a) At the low number of animals used. b) the different techniques employed. c) For the different patterns used. d) In the absence of a threshold value. We have tried to eliminate this potential error, to determine the Frog Macho (Bufo spinolosus limensis) of Lima and vicinity, the minimum dose capable of producing positive biological response using 420 animals, using as standards all commercial products Gonadotropin Chorionic that there are exac...
13
tesis de grado
Publicado 2017
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Objetivo: Demostrar que el uso previo de antibiótico es factor asociado para Infección del Tracto Urinario por enterobacterias productoras de BLEE en hospitalizados en el servicio de medicina del Hospital Regional Docente de Trujillo en el periodo de Enero 2016 a Diciembre 2016. Material y Métodos: Se llevó a cabo un estudio analítico, observacional, retrospectivo, de casos y controles. La muestra de la población de estudio estuvo constituida por 102 pacientes adultos que cumplieron con los criterios de inclusión y exclusión, distribuidos en dos grupos: 34 casos con urocultivos positivos para enterobacterias productoras de BLEE y 68 controles con urocultivos positivo para enterobacterias NO productoras de BLEE. Resultados: Se observó que la antibioticoterapia previa es factor asociado para ITU por enterobacterias productoras de BLEE mostrando un resultado significativo (OR=9,02 ...
14
artículo
Publicado 2013
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In this work the principal advances have been checked reached in the last twenty years, in the study of the fermentation of strains of Kluyveromyces marxianus for the production of the enzyme inulinase from inulin pure and sources of inulina and the determination of the properties and characteristics of the enzyme. With the aim of gathering and clarifying the greater knowledge of this one area, the investigation of this matter has been divided into three principal parts; substrate sources of inulin; microbial production of inulinase and characterization and properties of the inulinase. There is discussed the existing information about the microorganisms capable of producing inulinase those who have been brought by information of performance and especially the conditions of fermentation of strains of Kluyveromyces marxianus for the production of inulinase from several vegetable sources of...
15
artículo
Publicado 2013
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In this work the principal advances have been checked reached in the last twenty years, in the study of the fermentation of strains of Kluyveromyces marxianus for the production of the enzyme inulinase from inulin pure and sources of inulina and the determination of the properties and characteristics of the enzyme. With the aim of gathering and clarifying the greater knowledge of this one area, the investigation of this matter has been divided into three principal parts; substrate sources of inulin; microbial production of inulinase and characterization and properties of the inulinase. There is discussed the existing information about the microorganisms capable of producing inulinase those who have been brought by information of performance and especially the conditions of fermentation of strains of Kluyveromyces marxianus for the production of inulinase from several vegetable sources of...
16
tesis de grado
Publicado 2023
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Determinar los factores de riesgo para infección de tracto urinario (ITU) por gérmenes productores de betalactamasas de espectro extendido (BLEE) en pacientes pediátricos en el Hospital Belén de Trujillo, en el periodo enero 2014 a febrero 2020. MATERIAL Y MÉTODOS: Investigación observacional, analítica de caso control, retrospectiva, de nivel básico. La muestra comprendió 123 pacientes pediátricos hospitalizados, de ellos 41 casos (con ITU causado por gérmenes productores de BLEE) y 82 controles (con ITU causado por gérmenes no productores de BLEE). Se realizó la prueba Chi-cuadrado, se calculó el Odds Ratio (OR). Se utilizó el programa SPSS 25. RESULTADOS: El Sexo femenino (OR=2.727 [1.207-6.163], p=0.014) y la procedencia urbana (OR=2.852 [1.312-6.20], p=0.007) resultaron factores epidemiológicos de riesgo de ITU por gérmenes productores de BLEE; en lo referente a los...
17
tesis de grado
Publicado 2020
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El incremento de la frecuencia de enterobacterias productoras de β-lactamasas de espectro extendido (BLEE) representan una amenaza grave tanto para la práctica clínica veterinaria como para la salud pública. En este contexto, se sugiere que los perros son reservorios y potenciales transmisores de estos patógenos al humano. El objetivo de este estudio es determinar la presencia de Escherichia coli, Klebsiella spp. y Enterobacter spp. BLEE en perros sanos de la Clínica Veterinaria Docente Cayetano Heredia (San Martín de Porres, Lima). Muestras fecales de perros sanos (n=58) fueron sembradas en agar de cultivo cromogénico HiCrome™ ESBL. Las cepas aisladas fueron sometidas a pruebas bioquímicas y sembradas en agar McConkey. Posteriormente, la confirmación de cepas productoras de BLEE se realizó mediante la técnica de sinergia de doble disco (DDST) descrita por “El Comité Eur...
18
artículo
The aim of this study was to characterize moderately halophilic bacteria producing hydrolases of biotechnological interest. For that, 32 aerobic bacterial isolates were selected from salt environments such as Pilluana, Huacho, Maras, Chilca, Paracas and Ventanilla. The morphological and physiological properties and hydrolytic capacity to various substrates were determined for all isolates. Genotypic characterization was performed by amplification of bacterial 16S ribosomal gene and cutting with the Hae III enzyme. The isolates 23, 2, 24, 19, 2 and 6 showed hydrolytic activity for Tween 80, olive oil, starch, casein, lactose and DNA, respectively. Isolates L3CH and L2PAR hydrolyzed the most substrates, except for lactose and DNA, in contrast isolates P2RI-17 and M17 which only hydrolyze Tween 80. Twelve genetic profiles were obtained from the restriction analysis of amplified 16S ribosoma...
19
artículo
The aim of this study was to characterize moderately halophilic bacteria producing hydrolases of biotechnological interest. For that, 32 aerobic bacterial isolates were selected from salt environments such as Pilluana, Huacho, Maras, Chilca, Paracas and Ventanilla. The morphological and physiological properties and hydrolytic capacity to various substrates were determined for all isolates. Genotypic characterization was performed by amplification of bacterial 16S ribosomal gene and cutting with the Hae III enzyme. The isolates 23, 2, 24, 19, 2 and 6 showed hydrolytic activity for Tween 80, olive oil, starch, casein, lactose and DNA, respectively. Isolates L3CH and L2PAR hydrolyzed the most substrates, except for lactose and DNA, in contrast isolates P2RI-17 and M17 which only hydrolyze Tween 80. Twelve genetic profiles were obtained from the restriction analysis of amplified 16S ribosoma...
20
artículo
Publicado 2013
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Hay pocos estudios regionales haciendo una relación del tipo de magma y sus respectivas ocurrencias metálicas. Durante la formación de magmas se desarrollan rocas productoras (“fértiles”) y no productoras (“estériles”). Es importante entonces, determinar qué controló las variaciones en las firmas o huellas geoquímicas de estas rocas, interpretar eventos magmáticos en tiempo-espacio para de esta forma focalizar la mineralogía en la zona estudiada En el Proyecto Esperanza en base de la caracterización geoquímica de los eventos ígneos representados por el stock monzodíoritico, diques y lavas andesíticas, stock y diques dacíticos se concluye que son de ambiente geotectónico de arco volcánico en zonas de subducción. La monzodiorita es la de más antigüedad, la que presenta mayor grado de oxidación y proveniente de magmas húmedos y la...
