The dosage Chorionic Gonadotropin, following the technique of Mainini Galli, is a simple and quick laboratory test, others whose clinical significance is emphasized. To perform the dosoie Chorionic Gonadotropin, following the above technique, it is necessary to know the minimum dose capable of producing positive biological response in the male toad. 1 The values ​​given in this figure, the different authors vary, and this variation is due to: a) At the low number of animals used. b) the different techniques employed. c) For the different patterns used. d) In the absence of a threshold value. We have tried to eliminate this potential error, to determine the Frog Macho (Bufo spinolosus limensis) of Lima and vicinity, the minimum dose capable of producing positive biological response using 420 animals, using as standards all commercial products Gonadotropin Chorionic that there are exac...