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1
artículo
Publicado 2019
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The aim of this study was to characterize the PepT1 of pirarucu Arapaima gigas through genomic and proteomic tools. A transcriptomic and proteomic analysis was made from sections of the intestine, spleen, liver and kidney of fingerlings. The transcriptomic analysis allowed obtaining two consensus sequences of 357 and 459 bp. Both fragments showed a high degree of homology (78 and 80%), mainly with nucleotide sequences coding for the PepT1 of Scleropages formosus of the same Order as A. gigas. The proteomic analysis by double mass spectrometry MALDI TOF/TOF allowed to identify 15 peptide sequences of PepT1 in pirarucu. In conclusion, PepT1 was partially characterized in the intestine of pirarucu, which could be used for further studies on the evaluation of its expression.
2
artículo
Publicado 2019
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The aim of this study was to characterize the PepT1 of pirarucu Arapaima gigas through genomic and proteomic tools. A transcriptomic and proteomic analysis was made from sections of the intestine, spleen, liver and kidney of fingerlings. The transcriptomic analysis allowed obtaining two consensus sequences of 357 and 459 bp. Both fragments showed a high degree of homology (78 and 80%), mainly with nucleotide sequences coding for the PepT1 of Scleropages formosus of the same Order as A. gigas. The proteomic analysis by double mass spectrometry MALDI TOF/TOF allowed to identify 15 peptide sequences of PepT1 in pirarucu. In conclusion, PepT1 was partially characterized in the intestine of pirarucu, which could be used for further studies on the evaluation of its expression. © 2019 Universidad Nacional Mayor de San Marcos. All rights reserved.
3
artículo
Publicado 2015
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In 65 normal pregnant protein iodine numbers are determined via the technique of chloric acid. In the first trimester of pregnancy is an average value of 8.82 ug%; in the second quarter 10 ug% in the third quarter and 11.99 ug%; These elevations Iodine protein in normal pregnancy as due to increased estrogen, in turn, cause an increase in transport capacity of the thyroid hormone binding proteins, not increasing the free thyroxine are interpreted, which explains the absence of clinical symptoms of hyperthyroidism.
4
artículo
Publicado 2015
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In 65 normal pregnant protein iodine numbers are determined via the technique of chloric acid. In the first trimester of pregnancy is an average value of 8.82 ug%; in the second quarter 10 ug% in the third quarter and 11.99 ug%; These elevations Iodine protein in normal pregnancy as due to increased estrogen, in turn, cause an increase in transport capacity of the thyroid hormone binding proteins, not increasing the free thyroxine are interpreted, which explains the absence of clinical symptoms of hyperthyroidism.
5
artículo
Publicado 2021
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The global spread and lethality of SARS-CoV-2 prompt the urgent need to develop an appropriate therapeutic strategy to control COVID-19 cases. The development of new drugs to fight this novel virus is urgent due to its rapid spread. Parallel alternatives have been proposed by using drugs already available for similar purposes. This review article describes theantiviral potential of ivermectin as well as its mechanisms of action against some viruses, and discusses its probable use to fight SARS-CoV-2.
6
artículo
Los estudios pioneros de Framingham mostraron una relación inversa entre los niveles bajos de HDL Colesterol y la morbilidad coronaria. Los ensayos con modificaciones en el estilo de vida han mostrado un muy discreto incremento en las cifras de HDL-C, y la intervención farmacológica con fibratos o acido nicotínico (niacina) no ha mostrado tampoco disminución de la mortalidad. Tratar a pacientes con medicamentos que afectan el transporte reverso de colesterol y que aumentan considerablemente la cifra de HDL tampoco ha logrado disminuir la morbilidad. La asociación inversa de cifras altas de HDL y mortalidad coronaria está siendo cuestionada en los últimos años y se ha comenzado a investigar la calidad del HDL o la de sus subfracciones como explicación a su actividad antiaterosclerótica.
7
artículo
Most Vibrio species identification methods take many days to deliver results, therefore it is important that research can be done on rapid and accurate detection and identification techniques. MALDI-TOF MS has proven to be a simple tool to identify and differentiate pathogens. The objective of this research was to recognize the available bibliographic information on the identification of Vibrio cholerae species non 01 non 139 of environmental origin by MALDI TOF MS. The Vibrio genera includes more than thirty species found in aquatic environments, some species cause diseases in both marine and human species. One of these organisms is V. cholerae, one of the most important species due to the production of cholera toxin (CT) which interrupts the transport of ions in the cells of the intestinal epithelium. The number of case reports involving V. cholerae no 01 no 139 in extraintestinal infe...
8
artículo
Los estudios pioneros de Framingham mostraron una relación inversa entre los niveles bajos de HDL Colesterol y la morbilidad coronaria. Los ensayos con modificaciones en el estilo de vida han mostrado un muy discreto incremento en las cifras de HDL-C, y la intervención farmacológica con fibratos o acido nicotínico (niacina) no ha mostrado tampoco disminución de la mortalidad. Tratar a pacientes con medicamentos que afectan el transporte reverso de colesterol y que aumentan considerablemente la cifra de HDL tampoco ha logrado disminuir la morbilidad. La asociación inversa de cifras altas de HDL y mortalidad coronaria está siendo cuestionada en los últimos años y se ha comenzado a investigar la calidad del HDL o la de sus subfracciones como explicación a su actividad antiaterosclerótica.
9
artículo
Publicado 2025
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The chame Dormitator latifrons is a symbolic species associated with rural aquaculture in coastal regions of the South American Pacific. Its adaptation to stay alive out of water (anhydrobiosis) for days is beneficial for its storage and transport at room temperature. The aim of the study was to determine the tolerance of chame to anhydrous conditions. Sixty chames were distributed in containers and kept in two climate-controlled environments at 16 and 24 °C for 96 h. Survival, malondialdehyde levels, total proteins and pH in the muscles and lactate dehydrogenase (LDH) activity in the gills were monitored. Additionally, the behaviour of the fish was monitored, as well as the total number of microorganisms with a sensory evaluation. The critical period of survival occurred after 48 h, where the pH decreased, and the MDA levels increased. The number of microorganisms increased in both en...
10
tesis de maestría
Publicado 2025
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La neurocisticercosis es una enfermedad parasitaria del SNC que causa epilepsia adquirida y es generada por la forma larvaria del cestodo Taenia solium. Es de curso crónico y en pacientes sintomáticos se caracteriza por convulsiones y epilepsia. Estudios en ratas de laboratorio con NCC, reportan daño axonal caracterizado por el acúmulo de proteínas (neurofilamento, APP) en forma de hinchazones axonales, que podría estar relacionado con deterioro en el transporte axonal. Objetivo: Evaluar el patrón de inmunorreactividad de las proteínas motoras kinesina y dineína como marcadores de daño en el transporte axonal en tejido cerebral de ratas Holtzman con NCC tratadas y no tratadas con antiparasitarios; y su asociación con el daño axonal. Metodología: Se utilizó tejido cerebral de ratas con NCC tratadas y no tratadas con antiparasitarios y ratas sanas sacrificadas a diferentes ti...
11
libro
Publicado 2005
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RESUMEN Athrough your brain, voracious killers are coursing through your veins, and corrosive chemicals sizzle in bubbles from your head to your toes. In fact, your entire body is like an electrical company, chemical factory, transportation grid, communications network, detoxification facility, hospital, and battlefield all rolled into one. The workers in each of these industries are your cells. Cells are the smallest form of life—the functional and structural units of all living things. Your body contains trillions of cells, organized into more than 200 major types.
12
Publicado 2019
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El objetivo de este trabajo de investigación fue las características estructurales de la célula en general son la individualidad, la sistematicidad y la tridimensionalidad. Las células contienen citosol y material genético y poseen enzimas y diversas proteínas. La membrana celular es una estructura formada por una bicapa lipídica que envuelve y delimita a la célula en su conjunto. El núcleo celular es un orgánulo membranoso en forma de esfera, localizado por lo general en la zona central de las células eucariotas. Contiene la mayor parte de la carga genética celular bajo la forma de moléculas muy largas de ADN. Estructuralmente, el núcleo celular consta de envoltura nuclear, poros nucleares, nucléolo, lámina nuclear, cromatina y cromosoma, nucleoplasma, otros cuerpos subnucleares (cuerpos de Cajal, PIKA, cuerpos PML, paraspeckles, speckles, cuerpos de escisión, cuerpos D...
13
artículo
Publicado 2022
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The aim of this study was to evaluate the sedative effect of three products: valerian extract (Valeriana officinalis), benzocaine and tricaine methanesulfonate (MS-222) on stress levels produced by a simulated transport model in tilapia fingerlings. In a first part, the sedative dose of the products was determined based on the clinical characteristics that indicate depression of the central nervous system. In the second part, sedation and subsequent simulated transport for 3 hours were performed. The fish were euthanized, and blood samples were taken from the tail vein to determine cortisol (ELISA) and glucose (portable glucometer) levels, haematological parameters and mortality. The optimal doses of sedation were 200, 35 and 75 mg/l, respectively, for valerian extract, benzocaine and MS-222. After 3 h of simulated transport, cortisol and glucose values were similar or higher compared to...
14
tesis doctoral
Publicado 2025
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Universidad Nacional Agraria La Molina. Escuela de Posgrado. Doctorado en Ciencia Animal
15
artículo
Publicado 2019
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El objetivo del estudio fue caracterizar el PepT1 del paiche Arapaima gigas mediante herramientas genómicas y proteómicas. Se realizó un análisis transcriptómico y proteómico a partir de secciones del intestino, bazo, hígado y riñón de alevines. El análisis transcriptómico permitió obtener dos secuencias consenso de 357 y 459 pb. Ambos fragmentos presentaron un alto grado de homología (78 y 80%), principalmente con secuencias de nucleótidos codificantes del PepT1 de Scleropages formosus del mismo Orden que el paiche. El análisis proteómico por espectrometría de doble masa MALDI TOF/TOF permitió identificar quince secuencias peptídicas del PepT1 en paiche. Como conclusión se logró caracterizar parcialmente el PepT1 en el intestino del paiche, las cuales podrán ser usadas para posteriores estudios sobre la evaluación de su expresión.
16
tesis doctoral
Publicado 2020
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Descargue el texto completo en el repositorio institucional de la Universidade Estadual de Campinas: https://hdl.handle.net/20.500.12733/1641647
17
tesis de maestría
Publicado 2019
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Universidad Nacional Agraria La Molina. Escuela de Posgrado. Maestría en Nutrición
18
artículo
The aim of this study was to determine the postnatal development of Islets of Langerhans (IL) in alpacas of 0 to 45 days of age. The animals were divided in nine age groups with range of five days. The mumber of IL varied from 3.03 to 4.46 in 10x fields (with an ocular of 10x), the IL diameter varied from 67.89 to 72.55 μm, the number of β cells in the IL from 23.79 to 47.08 and other cells from 18.15 to 39.93. Moreover, the insulin receptor and the transport protein GLUT-2 were observed by immunohistochemistry on the first day of life. It is concluded that the number and size of IL increased with age (p<0.05) while β cells do not show the same trend.
19
artículo
The aim of this study was to determine the postnatal development of Islets of Langerhans (IL) in alpacas of 0 to 45 days of age. The animals were divided in nine age groups with range of five days. The mumber of IL varied from 3.03 to 4.46 in 10x fields (with an ocular of 10x), the IL diameter varied from 67.89 to 72.55 μm, the number of β cells in the IL from 23.79 to 47.08 and other cells from 18.15 to 39.93. Moreover, the insulin receptor and the transport protein GLUT-2 were observed by immunohistochemistry on the first day of life. It is concluded that the number and size of IL increased with age (p<0.05) while β cells do not show the same trend.
20
tesis de grado
Publicado 2021
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Universidad Nacional Agraria La Molina. Facultad de Zootecnia. Departamento Académico de Producción Animal
