The aim of this study was to determine the frequency of extramedullary plasmacytomas (EMPs) in canines and felines diagnosed in the Histopathology Laboratory of Universidad Nacional Mayor de San Marcos in the period 2006-2016. Records of histopathological diagnosis related to neoplasms were located (n=1758), of which 1703 corresponded to canines and 55 to felines. Of these, 110 (6.5%) and 8 (14.5%) were EMPs in canines and felines, respectively. The frequency of EMPs was determined with respect to the species (canine/feline), sex (male/female), age (<2, ˃2-˂7,> 7 for canines, and <1, ˃1-˂7, > 7 for cats) and breed. A frequency of EMPs of 6.5% was found in canines and 14.5% for felines in relation to the total neoplasms. Likewise, it occurred in 65 and 63% male canines and felines, respectively. Regarding a...

            The aim of this study was to determine the frequency of extramedullary plasmacytomas (EMPs) in canines and felines diagnosed in the Histopathology Laboratory of Universidad Nacional Mayor de San Marcos in the period 2006-2016. Records of histopathological diagnosis related to neoplasms were located (n=1758), of which 1703 corresponded to canines and 55 to felines. Of these, 110 (6.5%) and 8 (14.5%) were EMPs in canines and felines, respectively. The frequency of EMPs was determined with respect to the species (canine/feline), sex (male/female), age (<2, ˃2-˂7,> 7 for canines, and <1, ˃1-˂7, > 7 for cats) and breed. A frequency of EMPs of 6.5% was found in canines and 14.5% for felines in relation to the total neoplasms. Likewise, it occurred in 65 and 63% male canines and felines, respectively. Regarding a...

The aim of this study was to apply the criteria of the World Health Organization (WHO) in the histopathological classification of canine lymphoma on cases diagnosed from 1996 to 2012 at the veterinary pathology laboratory of San Marcos University, Lima, Peru. A total of 1778 reports of canine neoplasia were revised and 16 cases with histopathological diagnoses of canine lymphoma were studied. The following types of lymphoma were identified: diffuse large B cell lymphoma (7/16) (immunoblastic subtype [4/16], centroblastic subtype [2/16], rich subtype T cells [1/16]), mantle cell lymphoma (3/ 16), peripheral T-cell lymphoma not otherwise specified (2/16), precursor B/T lymphoblastic lymphoma (2/16), anaplastic large cell lymphoma (1/16) and mycosis fungoides (1/16).

The present study reports a case of hydrometra in a female canine of 12 years who was taken to the Small Animals Clinic of the Faculty of Veterinary Medicine of the Universidad Nacional Mayor de San Marcos (Lima, Peru). The bitch had clinical signs of apparent gestation of 45 days, but no history of oestrus or mounts. Vital signs, uro-analysis, haematology, and blood biochemistry indicated values within normal parameters. The ultrasound examination showed compatible signs with hydrometra. An ovariohysterectomy was performed, and uterine samples were taken for histopathology, which confirmed the diagnosis, in addition to a concomitant cystic endometrial hyperplasia in one of the uterine horns.

The aim of this study was to apply the criteria of the World Health Organization (WHO) in the histopathological classification of canine lymphoma on cases diagnosed from 1996 to 2012 at the veterinary pathology laboratory of San Marcos University, Lima, Peru. A total of 1778 reports of canine neoplasia were revised and 16 cases with histopathological diagnoses of canine lymphoma were studied. The following types of lymphoma were identified: diffuse large B cell lymphoma (7/16) (immunoblastic subtype [4/16], centroblastic subtype [2/16], rich subtype T cells [1/16]), mantle cell lymphoma (3/ 16), peripheral T-cell lymphoma not otherwise specified (2/16), precursor B/T lymphoblastic lymphoma (2/16), anaplastic large cell lymphoma (1/16) and mycosis fungoides (1/16).

El presente estudio reporta un caso de hidrometra en un canino hembra de 12 años que fue llevada a la Clínica de Animales Menores de la Facultad de Medicina Veterinaria de la Universidad Nacional Mayor de San Marcos (Lima, Perú). La perra presentaba signos clínicos de gestación aparente de 45 días, pero sin historia de celo o monta. Las constantes vitales, uroanálisis, hematología y bioquímica sanguínea indicaron valores dentro de los parámetros normales. En el examen ecográfico se observaron signos compatibles con hidrometra. Se llevó a cabo una ovariohisterectomía y se tomaron muestras uterinas para histopatología, lo cual confirmó el diagnóstico; además de una hiperplasia endometrial quística concomitante en uno de los cuernos uterinos.

The objective of the present study was to monitor the detection of the Epizootic Hematopoietic Necrosis virus (EHNv) in trouts (Oncorhynchus mykiss) in fish farms of the highlands of Peru. Samples were taken from 111 diseased fish (liver, spleen, anterior kidney) from fish farms in the regions of Ancash, Junín and Huancavelica, Peru. The tissue samples were processed immediately for bacterial isolation and another group of samples were stored at -196 °C for PCR. The DNA of each tissue was extracted by the Trizol method and then purified with silica membranes (PureLink Genomic DNA kit). The PCR was performed using the MCP-1 primers specific for the EHNv, following the methodology proposed by the OIE and with the commercial kit VetPCRTM EHNV Detection for confirmation. Bacterial isolation was carried out by culturing on TSA and Anacker- Ordal agar (Cytophaga agar) of each tissue, and the...

The objective of the present study was to monitor the detection of the Epizootic Hematopoietic Necrosis virus (EHNv) in trouts (Oncorhynchus mykiss) in fish farms of the highlands of Peru. Samples were taken from 111 diseased fish (liver, spleen, anterior kidney) from fish farms in the regions of Ancash, Junín and Huancavelica, Peru. The tissue samples were processed immediately for bacterial isolation and another group of samples were stored at -196 °C for PCR. The DNA of each tissue was extracted by the Trizol method and then purified with silica membranes (PureLink Genomic DNA kit). The PCR was performed using the MCP-1 primers specific for the EHNv, following the methodology proposed by the OIE and with the commercial kit VetPCRTM EHNV Detection for confirmation. Bacterial isolation was carried out by culturing on TSA and Anacker- Ordal agar (Cytophaga agar) of each tissue, and the...

This study aimed to determine the possible presence ofPiscirickettsia salmonis in farmed rainbow trout (Oncorhynchus mykiss) in the Mantaro river basin, Junin, Peru, by the indirect inmunofluorescence test (IFAT). Kidney and spleen samples were collected from 180 trout from three fish farms. All samples resulted negative to P. salmonis; however, some individuals showed clinical sings of disease as skin ulcer, melanosis, abdominal swelling and exophthalmoses. Also, in the internal organs was found petechial hemorrhage in liver, pyloric caeca and spleen, splenomegaly and pale liver.

The aim of this study was to determine the presence of infectious agents by microbiological and histopathological studies in the neon tetra (Paracheirodon innesi), an Amazonian ornamental fish. A total of 168 fish were randomly collected from the aquarium of an ornamental fish retail collector in Iquitos, Peru. For the microbiological study, kidney and spleen samples were collected during the necropsy of 84 specimens, and bacteria were isolated in trypticase soy agar (TSA), Citophaga (AO) and Middlebrook 7H9, while identification was done through Gram staining and biochemical analysis. The histopathological study was conducted in the remaining 84 fish. Bacteria isolated were of genera Flavobacterium, Staphylococcus, Carnobacterium, Pseudomonas, Enterococcus, Bacillus and of the Enterobacteriaceae family. In gills was observed laminar hyperplasia, fusion of lamellae and Mixosporidia spore...

The aim of this study was to determine the presence of bacterial agents and to characterize the histopathological lesions in ornamental tetra bleeding heart (Hyphessobrycon erytrhostigma) fish from a commercial aquarium in the city of Iquitos, Peru. For the microbiological study 60 fish were used, samples were taken from spleen and kidney. Isolation was done on MacConkey agar, Trypticase Soy agar, Pseudomonas Aeromonas selective agar (GSP) and Cytophaga agar and the genus identification was done by Gram staining and biochemical tests. Another 60 fish were used for the histopathological study where the skin, eye, gills, stomach, intestine, liver, spleen, muscle, kidney and peritoneum tissues were analyzed. Seven bacterial genera were isolated: Pseudomonas, Aeromonas, Lactobacillus, Bacillus, Flavobacterium, Staphylocccus and Escherichia. Spores of Mixosporidium sp, hyperplasia and lamella...

The aim of this study was to identify the angiotensin II receptor antigens in the umbilical artery of the alpaca (Vicugna pacos). Six gravid uteri of alpacas from community farms located at 4000 m above the sea level in Cusco, Peru were used. The gestation time was estimated by the biparietal diameter of the foetus. Histological sections of 5 µm thickness were obtained from each umbilical artery. The Avidin-Biotin-peroxidase immunohistochemistry (IHQ) technique was applied, using IHQ reagents and polyclonal anti-Angiotensin II antibodies. The slides were analysed with a Leica DM750 binocular microscope with digital camera attached and LAS 4.7 Leica Macrosystems software. The intensity of marking was classified qualitatively (0: negative; 1: weak; 2: moderate; 3: strong labelling). For the positive control, ovine umbilical artery samples were used. The angiotensin II receptor antigens we...

El objetivo de este estudio fue determinar la posible presencia de Piscirickettsia salmonis en truchas de cultivo (Oncorhynchus mykiss) en la cuenca del río Mantaro, Junín, Perú, mediante la prueba de inmunofluorescencia indirecta (IFI). Se obtuvieron 180 truchas en forma aleatoria de tres piscigranjas, se beneficiaron, se les realizó la necropsia y se recolectaron muestras de riñón y bazo. Los tejidos fueron sometidos a la prueba de inmunofluorescencia indirecta (IFI). Todas las muestras resultaron negativas a P. salmonis; sin embargo, se encontraron peces con signos clínicos de enfermedad como úlceras en la piel, melanosis, abultamiento del abdomen y exoftalmia; asimismo, en órganos internos se encontraron hemorragias petequiales en hígado, ciegos pilóricos y grasa abdominal, esplenomegalia y palidez hepática.

The aim of this study was to determine the presence of infectious agents by microbiological and histopathological studies in the neon tetra (Paracheirodon innesi), an Amazonian ornamental fish. A total of 168 fish were randomly collected from the aquarium of an ornamental fish retail collector in Iquitos, Peru. For the microbiological study, kidney and spleen samples were collected during the necropsy of 84 specimens, and bacteria were isolated in trypticase soy agar (TSA), Citophaga (AO) and Middlebrook 7H9, while identification was done through Gram staining and biochemical analysis. The histopathological study was conducted in the remaining 84 fish. Bacteria isolated were of genera Flavobacterium, Staphylococcus, Carnobacterium, Pseudomonas, Enterococcus, Bacillus and of the Enterobacteriaceae family. In gills was observed laminar hyperplasia, fusion of lamellae and Mixosporidia spore...

The aim of this study was to determine the presence of bacterial agents and to characterize the histopathological lesions in ornamental tetra bleeding heart (Hyphessobrycon erytrhostigma) fish from a commercial aquarium in the city of Iquitos, Peru. For the microbiological study 60 fish were used, samples were taken from spleen and kidney. Isolation was done on MacConkey agar, Trypticase Soy agar, Pseudomonas Aeromonas selective agar (GSP) and Cytophaga agar and the genus identification was done by Gram staining and biochemical tests. Another 60 fish were used for the histopathological study where the skin, eye, gills, stomach, intestine, liver, spleen, muscle, kidney and peritoneum tissues were analyzed. Seven bacterial genera were isolated: Pseudomonas, Aeromonas, Lactobacillus, Bacillus, Flavobacterium, Staphylocccus and Escherichia. Spores of Mixosporidium sp, hyperplasia and lamella...

The aim of this study was to identify the angiotensin II receptor antigens in the umbilical artery of the alpaca (Vicugna pacos). Six gravid uteri of alpacas from community farms located at 4000 m above the sea level in Cusco, Peru were used. The gestation time was estimated by the biparietal diameter of the foetus. Histological sections of 5 µm thickness were obtained from each umbilical artery. The Avidin-Biotin-peroxidase immunohistochemistry (IHQ) technique was applied, using IHQ reagents and polyclonal anti-Angiotensin II antibodies. The slides were analysed with a Leica DM750 binocular microscope with digital camera attached and LAS 4.7 Leica Macrosystems software. The intensity of marking was classified qualitatively (0: negative; 1: weak; 2: moderate; 3: strong labelling). For the positive control, ovine umbilical artery samples were used. The angiotensin II receptor antigens we...

The objective of the study was to determine the frequency of hepatic lipidosis in rainbow trout (Oncorhynchus mykiss) of juvenile phase based on anatomical and histopathological lesions in the liver. The specimens (n=58) were obtained from a commercial fish farm in the central highlands of Peru. The fish were classified as healthy (n=23) and ill (n=35) according to the darkening of the skin and fin erosion. Liver samples were collected, fixed in 10% formalin, and stained with hematoxylin eosin. Results showed that 70.6% (41/58) of the fish had lesions compatible with hepatic lipidosis, where grade 3 had the largest incidence (27.6%). The highest frequency of hepatic lipidosis in grade 3 and 4 was observed in healthy animals.

The purpose of this study was to determine the frequency of canine rhabdomyosarcoma based on its histopathological classification, that were diagnosed in the histopathology laboratory of the Veterinary Faculty of San Marcos University, Lima, Peru in the period of January 2000 to December 2008. A total of 63 cases of canine rhabdomyosarcoma were diagnosed out of 1125 canine neoplasic tumors (5.6 ± 1.3%). Among this, only 47 cases were possible to classify where 27 cases (57.4%) corresponded to embryonal rhabdomyosarcoma, 19 (40.4%) to alveolar rhabdomyosarcoma, and one case (2.1%) to pleomorphic rhabdomyosarcoma. The most frequent region of the body affected by the embryonal type was the head and neck, where 6 to 10 year-old animals were the most affected.

Persistent Mullerian duct (PMDS) is a type of hereditary male pseudohermaphroditism observed in several dog breeds. Males have testes and XY chromosomes in addition to oviduct cranial vagina, uterus and bilateral Müllerian duct derivatives. In the present study is described the case of a 8-year Schnauzer dog, phenotypically male, with a history of anorexia, lethargy and tenesmus during the last two days. The pet had male genitalia but evident right lateral cryptorchidism; however, ultrasound examination revealed that the animal had remnants of the duct Muller (uterine body and horns) with an infectious process compatible with pyometra. The blood smear showed polymorphonuclear cells without corpuscles of Barr. A tumor mass was found cranial to the right uterine horn and corresponded to a testicular neoplasia of Sertoli cells. The left testicle was located in the scrotal sac and was hypop...

El objetivo del estudio fue determinar la frecuencia de lipidosis hepática en la trucha arcoíris (Oncorhynchus mykiss) de fase juvenil en base a lesiones anátomohistopatológicas en el hígado. Se trabajó con 58 peces de una piscigranja comercial de la sierra central del Perú. Los peces fueron clasificados como sanos (n=23) y enfermos (n=35) según el oscurecimiento de la piel y erosión de las aletas. Se colectaron muestras de hígado, que se fijaron en formol al 10% y fueron coloreadas con Hematoxilina-eosina. El 70.6% (41/58) de los peces presentó lesiones compatibles con lipidosis hepática, siendo el grado 3 el de mayor presentación (27.6%). Las mayores frecuencias de lipidosis hepática en grados 3 y 4 se observaron en animales sanos.