The present paper describes the case of a two-year-old filly who developed severe gastrointestinal lesions after receiving prolonged doses of phenylbutazone when treating a fracture in the right forelimb. Necropsy findings revealed severe acute diffuse catarrahhemorrhagic-ulcerative gastritis and submucosal edema and severe diffuse acute mucohemorrhagic enterocolitis and splenomegaly with severe acute passive congestion. The cause of death was due to cardio-respiratory arrest and coagulopathy with circulatory disturbance and gastric ulcerogenic process associated with iatrogenic shock by phenylbutazone.

Feline Infectious Peritonitis (PIF) is a lethal disease in cats caused by a coronavirus, which until now has no curative treatment. Two clinical cases are described: a 6-month Siamese male with apathy and anorexia and a 19-month female with short hair who was presented with abortion and decay. Both patients presented mild to severe ascites with a yellowish and protein peritoneal fluid compatible with wet form of PIF. At necropsy ascites was found and fibrin gelatinoid masses adhered to the serosa of liver, spleen, pericardium, intestine and mesentery were observed in both patients. Histopathology showed copious mixed inflammatory exudate composed of fibrin strings interspersed with neutrophils at varying degrees of degeneration and plasma cells, accompanied by severe vasculitis with cytoplasmic swelling of endothelial cells and effusion of acidophilic proteinic fluid.

El presente trabajo describe el caso de una potranca de dos años que desarrolló graves lesiones gastrointestinales después de recibir dosis prolongadas de fenilbutazona al tratar una fractura en el miembro anterior derecho. Los hallazgos de necropsia revelaron una severa gastritis catarro- hemorrágico-ulcerativa difusa aguda y edema submucoso y severa enterocolitis mucohemorrágica difusa aguda y esplenomegalia con severa congestión pasiva aguda. La causa de muerte se debió a un paro cardio-respiratorio y coagulopatía con disturbio circulatorio y proceso ulcerogénico gástrico asociado a shock iatrogénico por fenilbutazona.

Feline Infectious Peritonitis (PIF) is a lethal disease in cats caused by a coronavirus, which until now has no curative treatment. Two clinical cases are described: a 6-month Siamese male with apathy and anorexia and a 19-month female with short hair who was presented with abortion and decay. Both patients presented mild to severe ascites with a yellowish and protein peritoneal fluid compatible with wet form of PIF. At necropsy ascites was found and fibrin gelatinoid masses adhered to the serosa of liver, spleen, pericardium, intestine and mesentery were observed in both patients. Histopathology showed copious mixed inflammatory exudate composed of fibrin strings interspersed with neutrophils at varying degrees of degeneration and plasma cells, accompanied by severe vasculitis with cytoplasmic swelling of endothelial cells and effusion of acidophilic proteinic fluid.

The anatomo histopathological characteristics of the intestine in rats with acute enteritis induced by injection of one dose of indomethacine and treated with metronizadole were evaluated. Male albino Holtzman rats (n=50) were used. They were 4 months old on average and 250-275 g of body weight. Animals were equally distributed in five groups: Control (Group 1), subcutaneously injected with indomethacine (15 mg/kg) (Group 2), and injected with indomethacine plus 1, 2 or 3 doses of metronizadole (50 mg/kg) (groups 3, 4 and 5, respectively). The first dose of metronizadole was orally administered 50 minutes before the indomethacine and subsequent doses were every 5 hours. The observation of lesions was done 48 hours later. Lesions were located in the mesenteric side of the medium and distal jejune. Macroscopic lesions were characterized by thickness and dilatation of the intestinal wall, l...

A surgery technique of gastroyeyunostomy was applied in dogs with experimental pyloric stenosis to observe the transit of chyle through the new path, and to evaluate the adverse effects on the animal. Ten crossbred dogs (5 female and 5 male) of 2-3 years old were surgically pyloric stenosed and the gastroyeyunostomy was performed. Animals were clinically evaluated for 35 days, and then, contrast radiographs were taken before necropsy. The post-surgery clinical observations shown an adequate recovery by day 10 after surgery. Vomit, an important clinical sign was evaluated. Animals were grouped as “bad” for recurrent vomits, “good” for occasional vomits, and “excellent” for animals apparently normal. 33% of dogs were considered as “good” and 67% as “excellent”. An adequate outflow of contrast liquid through the anastomosed organs was observed in all animals. There were ...

Se evaluaron las características anatómicas e histopatológicas del intestino de ratas con enteritis aguda inducida con una dosis de indometacina y tratadas con metronidazol. Se usaron 50 ratas albinas machos de la raza Holtzman, de 4 meses de edad promedio y de 250-275 g de peso vivo. Los animales se distribuyeron por igual en 5 grupos. El Lote 1 fue el lote control, el Lote 2 recibió únicamente la indometacina (15 mg/kg p.v. por vía sucutánea), y los lotes 3, 4 y 5 recibieron la indometacina, además de una, dos y tres dosis de metronizadol (50 mg/kg p.v. por vía oral), respectivamente. La primera dosis de metronizadol se administró 50 minutos previos a la indometacina, y las demás dosis con intervalos de 5 horas. La observación de las lesiones se realizó a las 48 horas. Todas las lesiones fueron halladas en el lado mesentérico del yeyuno medio y distal. Las lesiones macros...

Se expone la técnica quirúrgica de gastroyeyunostomía en caninos con estenosis pilórica experimental para observar el tránsito de quilo a través de esta vía alterna y evaluar los posibles efectos adversos que ocasione en el animal. Se utilizaron 10 caninos mestizos (5 hembras y 5 machos) de 2 a 3 años de edad, a los que se les estenosó el píloro utilizando una ligadura de seda y luego se les realizó la técnica quirúrgica de gastroyeyunostomía. Se evaluaron por 35 días post-operatorios, y al final se les tomó radiografías de contraste y se les practicó la necropsia. La observación clínica mostró que los animales se recuperaron dentro de los 10 días de la cirugía. El vómito, signo cardinal en los casos de estenosis pilórica fue evaluado, y los animales se agruparon por el análisis en base a este signo como “malos” si el vómito era recurrente, “buenos” si ...

The objective of the present study was to study the cytoarchitectural structure of the cervical and thoracic segments of thymus in alpaca (Lama pacos) fetuses. For this purpose, 10 fetuses between 40-190 days of gestation were collected form the local slaughterhouse. At day 40, the developing thymus has acinar cytoarchitectural morphology, infiltrated by a few lymphoid cells surrounded by mesenchymal cells. At day 60, the cervical segment looks like a «follicular» lymphoid, densely populated by thymocytes, whereas the thoracic region has a cystic appearance with short trabecular projections. Cortex and medulla differentiation starts in the posterior cervical and thoracic segments by day 100 and in the anterior cervical segments by day 130, and is well established by day 165. The thymic capsule remained as a double cellular layer of lax connective tissue. Subcapsular and trabecular bloo...

Fifteen Holtzman male rats weighing 250 to 275 g were distributed in four groups. Group 1 was subcutaneously injected with 5% NaHCO3 (n=20). Groups 2, 3 and 4 received 15 mg/kg of indomethacin subcutaneously (n=10 rats/group). Macroscopic and microscopic evaluation of small intestine (jejunum-ileum) were carried out at first, second and third day after drug administration. Intestinal injury were observed along the mesenteric side of the middle and distal jejunum. Macroscopic lesions consisted of bowel thickening and dilatation, absence of the mucosa, hyperemia and multiple longitudinal and round ulcers. These lesions were observed at 24 h after drug injection and were present by 72 h. There were no statistical differences (p>0.05) between groups 2, 3 and 4, relating to gross lesions. Microscopically, lesions were characterized by erosion of the mucosa, fibrinous and bacterial infiltra...

The aim of the study was to determine the frequency of neoplasms in the mammary gland of dogs. In this retrospective study (period of 1994-2006), records from the Patology Laboratory of the Veterinary Medicine Faculty, San Marcos University were used. There were a total of 4496 canine protocols of which 1128 were corresponded to neoplasms (25 ± 1.2%). Among them, 204 cases were in the mammary gland (18.1 ± 2.3%). Mammary neoplasms gland mainly occurred in females (94%) and in crossbred animals (38.3%). Dogs older than 10 years of age were most affected (46.4%). The inguinal pair was also the most affected (32.6%). Malignant neoplasms were more frequent (78%) than benign neoplasms. The adenocarcinoma was the most common neoplasm affecting the mammary gland (53.4%).

The aim of the present study was to determine the spleen cytoarchitecture in alpacafetuses of different stages of development. Sixteen alpaca fetuses were obtained fromfemale alpacas slaughtered in the central highlands of Peru. The fetuses were distributedin six groups according to their fetal age (55, 100, 120, 150, 180, and 200 days). Spleens were dissected and samples were stained with hematoxylin-eosin (cellular distribution),Masson ́s trichrome (collagen and muscle fibers), and periodic acid Schiff for argenticimpregnation (reticular fibers) for histological evaluation. Macroscopically, the spleen at55 days of age is slightly outlined, at 120 days is lobed with a triangular shape and at 150days had the appearance of the mature animal. Microscopically, at 55 days there is apredominance of transitional embryonic connective tissue to reticular tissue and somelymphoid cells are locate...

The aim of the present study was to determine the spleen cytoarchitecture in alpacafetuses of different stages of development. Sixteen alpaca fetuses were obtained fromfemale alpacas slaughtered in the central highlands of Peru. The fetuses were distributedin six groups according to their fetal age (55, 100, 120, 150, 180, and 200 days). Spleens were dissected and samples were stained with hematoxylin-eosin (cellular distribution),Masson ́s trichrome (collagen and muscle fibers), and periodic acid Schiff for argenticimpregnation (reticular fibers) for histological evaluation. Macroscopically, the spleen at55 days of age is slightly outlined, at 120 days is lobed with a triangular shape and at 150days had the appearance of the mature animal. Microscopically, at 55 days there is apredominance of transitional embryonic connective tissue to reticular tissue and somelymphoid cells are locate...

El objetivo del presente trabajo fue el estudio citoarquitectural de los segmentos cervicales y torácicos del timo en fetos de alpaca (Lama pacos). Se recolectaron 10 fetos entre 40 y 190 días de desarrollo para ser sometidos a estudio histológico. A los 40 días, el esbozo del timo tiene una morfología citoarquitectural de tipo acinar infiltrado por escasas células linfocíticas y rodeado por células mesenquimales. A los 60 días, el segmento cervical se presenta como un “folículo” linfoide densamente poblado por timocitos, mientras que la región torácica tiene una apariencia cavitaria con proyecciones trabeculares cortas. La diferenciación de corteza y médula se inicia en los segmentos cervicales posteriores y torácicos a los 100 días y en los segmentos cervicales anteriores a los 130 días. Todos los segmentos presentan disposición lobulillar bien definidos en cortez...

Se reprodujo un modelo experimental de enteritis aguda con una dosis subletal de indometacina en ratas albinas para caracterizar las lesiones macro y microscópicas en el yeyuno-íleon. Se utilizaron 50 ratas machos Holtzman, con un peso de 250-275 g distribuidas en 4 lotes. El lote 1 (control, n=20) recibió una inyección subcutánea de NaHCO3 al 5% y a los lotes 2, 3 y 4 (n=10 ratas/lote) se les aplicó indometacina (15 mg/kg de peso vivo), vía subcutánea. La evaluación macro y microscópica del intestino delgado (yeyunoíleon) se hizo el primer, segundo y tercer día de inyectada la droga. Las lesiones intestinales se ubicaron en el lado mesentérico del yeyuno medio y distal. Macroscópicamente se observó engrosamiento y dilatación de la pared intestinal, pérdida de la mucosa, hiperemia y múltiples úlceras lineales y circulares, que fueron evidentes dentro de las primeras 24...

El objetivo del estudio fue determinar la frecuencia de presentación de neoplasias en la glándula mamaria de caninos, diagnosticadas histopatológicamente en el Laboratorio Patología de la Facultad de Medicina Veterinaria de la Universidad de San Marcos, Lima, a través de un estudio retrospectivo que cubrió un periodo de 13 años (1994-2006). Se tuvieron 4496 protocolos para la especie canina, de los cuales 1128 correspondieron a procesos neoplásicos (25 ± 1.2%). Dentro de estos, 204 casos fueron de neoplasia en glándula mamaria (18.1 ± 2.3%). Las neoplasias mamarias se presentaron principalmente en hembras (94%) y en animales mestizos (38.3%). Los caninos mayores de 10 años de edad fueron los más afectados (46.4%). El par mamario más comprometido fue el inguinal (32.6%). Las neoplasias malignas fueron más frecuentes (78%) que las benignas. El adenocarcinoma fue el tipo de n...

The study aimed to determine the frequency of neoplasmas that were histopathologically diagnosed at the Pathology Laboratory of School of Veterinary Medicine, San Marcos University in the period 1996-2007. The retrospective study included 73 cases of neoplasms. Animals older than 9 years of age (49.3%) and females (72.9%) were the most affected. Tumors of epithelial origin were the most common type (53.5%), being mammary adenocarcinoma (21.1%) and lymphoma (16.9%) the most frequent.

A total of 48 intestinal samples of new born alpacas which died due to diarrhea processes were evaiuated. Samples collected during the dry season (June-August, 2002) were from animals belonging to the Experimental Research Statiori of IVITA, La Raya, Cnsco, and &ose collected during the pamtntion scason (Febmary, 2003) were from a fanners community inNuiioa, Puno. Topographic intestinal samples were tjken, fixed in 15% fomalinen solution and stained with H-E. Thiriy seven cases were compatible with bacteria1 omcesses. where 30 of k m were due to enterotoxemia and the other 7 were processes complicated with colibacilosis. Only two of these cases wereassociated with Cqptosporidirim sp. Four cases were related with Eimeria macusaniensis and enterotoxemia and ?cases wirh E. maarsanensis and E. prrnoensis. OnIy 4 cases were observed in association witb E. larnae. This is the first report ...

The aim of the study was to evaluate an immunohistochemical technique for the diagnosis of canine mixed mammary tumors (CMMT) using anti-cytokeratin AE1/AE3 and anti-vimentin monoclonal antibodies that are routinely used in the diagnosis of human mammary tumors. Twenty paraffin tissue blocks of canine mixed mammary tumors, previously diagnosed by hematoxilin-eosin (HE) histopathology were used. Three slides sets, 4 μm thick, were obtained per viability of the tissue, and the other two were used for the immunohistochemistry through the Streptavidin-Biotin-labeled method. Epithelial cells positively reacted against the anti-cytokeratin AE1/AE3 showing a brown colour at the cytoplasma level. Fibroblasts, connective tissue, myoepithelial cells, cartilage cells and bone areas showed a brown colour against the anti-vimentin antibody. All benign tumors reacted positively to both antibodies, wh...

Canine histopathological records from de Pathology Laboratory of the Universidad Nacional Mayor de San Marcos, Lima, during the period of 1995-2006 were evaluated for determining the frequency of neoplasias in relation to sex, age, breed, cellular origin, and localization. Out of 4438 canine records, 1092 corresponded to neoplasia (24.6%). Higher tumoral frequency was found in age groups of 5 to <9 (37.1%) and ≥9 (35.6%) years old. None differences were found due to sex, but the Boxer breed was the most affected (12.1%). Malignant neoplasias were most frequent (64.9%) than benign ones, where the transmissible venereal tumor was the most frequent benign tumor and the mammary adenocarcinoma was the most frequent malign tumor. Skin and subcutaneous tissue (39.5%) and mammary gland (16.7%) were the most common sites for neoplasias.