Pneumocystis jirovecii es el hongo oportunista a nivel pulmonar más común en personas con VIH/sida. El diagnóstico se basa tradicionalmente en la identificación microscópica de los quistes y trofozoítos en secreciones respiratorias. Sin embargo, con la introducción de la Reacción en Cadena de la Polimerasa (PCR) anidada el diagnóstico puede hacerse incluso en secreciones respiratorias obtenidas por métodos no invasivos (1). La PCR anidada en el caso de Pneumocystis amplifica el ADN mitocondrial a través de la detección del gen mtLSU rRNA que es una región altamente conservada. Esta es una prueba altamente sensible y reproducible pudiendo ser usada en diferentes muestras biológicas

Este es el reporte de un caso inusual de colitis pseudomembranosa con un desenlace fatal en el que se demostró la coexistencia de C. difficile y citomegalovirus en un paciente peruano con SIDA y compromiso gastrointestinal por una micobacteria.

Informe IREMAR; n° 19, 1963, .58 p.

Informe IREMAR; n° 7, 1963, 41 p.

Bacillus strains isolated from several stages of white asparagus production/processing before/after thermal treatment were characterized molecularly by BOX–PCR amplification and 16S ribosomal gene sequencing.

Perú boasts a wide diversity of fine aroma cocoa that merits quantifying its total phenols, anthocyanins, and characterize sensory the liquor and nibs of cacao, in two fermentation systems: traditional and micro-fermentation. The studying samples were “Chuncho”, Señorita variety, Achoccha, Pamuco and Común, and SHU-1 variety. For the quantification of total phenols was prepared a hydroalcoholic extract (100mg/mL). To the sensory characterization were chosen thirteen attributes whose results were subjected to multivariate analysis using the principal components method. The amount of total phenols was lower in the liquor compared to cocoa nibs, except in the microfermentation of Señorita and Achoccha variety, and in the traditional fermentation of Señorita variety; being the anthocyanin content higher compared to nibs. The cocoa liquor of Achoccha, Común and Señorita varieties i...

Perú boasts a wide diversity of fine aroma cocoa that merits quantifying its total phenols, anthocyanins, and characterize sensory the liquor and nibs of cacao, in two fermentation systems: traditional and micro-fermentation. The studying samples were “Chuncho”, Señorita variety, Achoccha, Pamuco and Común, and SHU-1 variety. For the quantification of total phenols was prepared a hydroalcoholic extract (100mg/mL). To the sensory characterization were chosen thirteen attributes whose results were subjected to multivariate analysis using the principal components method. The amount of total phenols was lower in the liquor compared to cocoa nibs, except in the microfermentation of Señorita and Achoccha variety, and in the traditional fermentation of Señorita variety; being the anthocyanin content higher compared to nibs. The cocoa liquor of Achoccha, Común and Señorita varieties i...

Cacao breeding programs focus in obtaining cacao varieties with important traits to both producers and consumers. A vital step for plant breeders is the characterization of the genetic material kept in germplasm banks in order to describe its level of diversity, and find interesting traits both morphologically and molecularly. In this way, the investigator will monitor how useful the accessions could be, determine duplications, and manage the bank appropriately. In this study, we aimed to test: the potential of ISSR (Interspread Sequence Repeats) markers to differentiate 46 cacao accessions maintained in Tingo Maria - Perú, and the similarity relationships between the accessions. In this research, the results showed that ISSR, despite its dominance nature, could establish eye-catching associations, such as, the grouping of Trinitario accessions into a common cluster

Cacao breeding programs focus in obtaining cacao varieties with important traits to both producers and consumers. A vital step for plant breeders is the characterization of the genetic material kept in germplasm banks in order to describe its level of diversity, and find interesting traits both morphologically and molecularly. In this way, the investigator will monitor how useful the accessions could be, determine duplications, and manage the bank appropriately. In this study, we aimed to test: the potential of ISSR (Interspread Sequence Repeats) markers to differentiate 46 cacao accessions maintained in Tingo Maria - Perú, and the similarity relationships between the accessions. In this research, the results showed that ISSR, despite its dominance nature, could establish eye-catching associations, such as, the grouping of Trinitario accessions into a common cluster

Cacao breeding programs focus in obtaining cacao varieties with important traits to both producers and consumers. A vital step for plant breeders is the characterization of the genetic material kept in germplasm banks in order to describe its level of diversity, and find interesting traits both morphologically and molecularly. In this way, the investigator will monitor how useful the accessions could be, determine duplications, and manage the bank appropriately. In this study, we aimed to test: the potential of ISSR (Interspread Sequence Repeats) markers to differentiate 46 cacao accessions maintained in Tingo Maria - Perú, and the similarity relationships between the accessions. In this research, the results showed that ISSR, despite its dominance nature, could establish eye-catching associations, such as, the grouping of Trinitario accessions into a common cluster

El estudio evalúa la calidad total en la atenci6n médica del policlínico Santa Rosa IPSS-Comas, definiendo a la calidad como conjunto de propiedades y características del servicio que satisfacen las necesidades o expectativas del paciente. La evaluación se realiza en base a variables Gerenciales con un enfoque de sistemas determinando indicadores de estructura (accesibilidad geográfica, econ6mica y de la organización y disponibilidad de recursos), proceso (actividades, grado de uso, productividad, etc.) y resultados (eficiencia, eficacia y calidad). Se elaboró un instrumento tipo encuesta el cual se aplic6 a una muestra aleatoria representativa de la poblaci6n seleccionada en forma estratificada de los usuarios al policlínico interrogándose sobre accesibilidad y calidad del servicio. En la evaluaci6n se identificaron problemas eventuales, denominados así cuando se comparan con...

The aim of this study was to determine the seroprevalence of Toxoplasma gondii in female llamas from two farms located in the Melgar province, Puno. A total of 284 blood samples were tested using the indirect immunofluorescence test (IFAT) to detect antibodies against T. gondii. The results showed that 47.5 + 5.8% (135/284) of the samples had antibodies and they increased with age. The seroprevalence from birth till 2 years, 2- 4 years, >4-6 years, and >6 years was 33.8 ± 11.0, 51.2 ± 10.8, 56.8 ± 14.6, and 50.6 ± 10.5%, respectively. There were significant differences (p<0.05) in the frecuency of female reactors between farms. Age and farm variables were considered as risk factors for the infection with Toxoplasma gondii (p<0.0001).

El objetivo del estudio fue determinar la seroprevalencia del Toxoplasma gondii en llamas hembras de dos fundos ganaderos de la provincia de Melgar, Puno. Se colectaron sueros sanguíneos de 284 animales para la detección de anticuerpos mediante el método de Inmunofluorescencia Indirecta (IFI). El 47.5 ± 5.8% (135/284) del total de las muestras presentaron anticuerpos. La frecuencia de reactores se incrementó en relación directa con la edad de los animales (nacimiento-2 años: 33.8 ± 11.0%; >2 a 4 años: 51.2 ± 10.8; >4 a 6 años: 56.8 ± 14.6%; y >6 años: 50.6 ± 10.5%). Se encontraron diferencias estadísticas (p<0.05) en la frecuencia de reactores entre fundos. Además, se halló que la variable edad y zona de muestreo representan un factor de riesgo para la infección con T. gondii (p<0.0001).

The agricultural sector faces risks from damage caused by phytopathogens, and many farmers rely on synthetic fungicides to combat them. However, excessive use of these products pollutes the environment and promotes pathogen resistance. This study evaluated the mycelial growth inhibition of 57 plant extracts against Botrytis cinerea, Lasiodiplodia theobromae, and Fusarium sp., and their efficacy in controlling Erysiphe necator in the field. It also evaluated their phytotoxic effect on wheat seeds and the identification of metabolites present in the extracts. The most effective extracts were those of Ambrosia artemisiifolia, Conyza sumatrensis, Dysphania ambrosioides, Minthostachis mollis, Salvia sp., Pimpinella anisum, and Syzygium aromaticum. The P. anisum extract exhibited the greatest inhibition of B. cinerea growth in tomatoes, while the most effective extracts against E. necator were...

Se presenta el caso de un paciente varón de 59 años de edad, sin antecedentes de interés, fumador de 4 cigarrillos/día y bebedor social. Ocho días antes de acudir a valoración sufre caída mientras subía las escaleras, con contusión tangencial contra la pared en zona lumbar (fosa renal) y glútea. Acude por aparición de lesión que va aumentando de tamaño y con cierto dolor. Se observa bultoma fluctuante en zona de fosa renal derecha, con aparente hematoma desde la pared lateral de abdomen superior hacia cresta ilíaca, además de hematomas en ambos glúteos. Se realiza ecografía en urgencias evidenciándose existencia de colección anecogénica de al menos 20×2×6,5 cm de diámetro máximo transversal, anteroposterior y craneocaudal. Se realiza drenaje bajo control ecográfico extrayendo 300 cc de contenido hemático; posterior control evidencia desaparición del mismo. La l...

OBJETIVO: El presente trabajo tiene como objetivo principal, evaluar la eficacia de la terapia triple con esomeprazol en la erradicación del Helicobacter pylori (Hp). METODOLOGÍA: Se realizó un estudio descriptivo, prospectivo en el Servicio de gastroenterología del Hospital Nacional Cayetano Heredia (HNCH), donde se seleccionaron pacientes dispépticos no ulcerosos con infección por Hp (determinada mediante biopsia) entre los meses de junio a agosto del 2008, a los cuales se les dividió en dos grupos: el ¨grupo control¨ se trató con amoxicilina 1g VO c/12h, claritromicina 500 mg VO c/12h y omeprazol 20 mg VO c/12h, y el ¨grupo en estudio¨ recibió amoxicilina 1g VO c/12h, claritromicina 500 mg VO c/12h y esomeprazol 20 mg VO c/12h, ambos esquemas se dieron por 10 días. A las 4 semanas de completado el tratamiento, a cada grupo se le hizo un control endoscópico con toma de b...

ObjetivosCaracterizar los niveles y mecanismos de resistencia a β-lactámicos, quinolonas y macrólidos en 62 aislamientos de Escherichia coli causantes de bacteriemia en niños peruanos.MétodosSe determinaron las concentraciones inhibitorias mínimas (CIM) de ciprofloxacino, ácido nalidíxico (NAL) y azitromicina en presencia y ausencia de Phe-Arg-β-naftilamida. También se estableció la susceptibilidad a otros 14 agentes antimicrobianos. Se identificaron las β-lactamasas de espectro extendido (ESBL) y se determinaron las mutaciones en gyrA y parC, así como la presencia de mecanismos transferibles de resistencia a quinolonas (TMQR) y macrólidos (TMMR).ResultadosCincuenta aislados (80,6%) eran multirresistentes. Se observaron altas proporciones de resistencia a la ampicilina (93,5%), al NAL (66,1%) y al trimetoprim/sulfametoxazol (66,1%). Ningún aislado mostró resistencia a los...

This study aimed to determine the clinical signs and pathological findings caused for Ornithobacterium rhinotracheale and Escherichia coli in White Leghorn layers under controlled conditions. A total of 80 replacement hens of five weeks were selected. Before starting the experiment, microbiological tests were conducted in 16 chicks to verify if they were exposed to pathogens. The remaining 64 chicks were distributed in four equal groups (n=16). On day 1 of the experiment G1 and G2 were inoculated via intranasal with 0.6 x 107 UFC/ml of O. rhinotracheale and G3 and G4 (negative control) received brain heart infusion broth. Forty eight hours later, G2 and G3 were inoculated via intranasal with 0.6 x 107 UFC of E. coli. Four chicks per group were slaughtered on days 3, 7, 14, and 21 after inoculation and samples were collected from paranasal sinuses, trachea, lung, air sacs and liver for op...

This study aimed to determine the clinical signs and pathological findings caused for Ornithobacterium rhinotracheale and Escherichia coli in White Leghorn layers under controlled conditions. A total of 80 replacement hens of five weeks were selected. Before starting the experiment, microbiological tests were conducted in 16 chicks to verify if they were exposed to pathogens. The remaining 64 chicks were distributed in four equal groups (n=16). On day 1 of the experiment G1 and G2 were inoculated via intranasal with 0.6 x 107 UFC/ml of O. rhinotracheale and G3 and G4 (negative control) received brain heart infusion broth. Forty eight hours later, G2 and G3 were inoculated via intranasal with 0.6 x 107 UFC of E. coli. Four chicks per group were slaughtered on days 3, 7, 14, and 21 after inoculation and samples were collected from paranasal sinuses, trachea, lung, air sacs and liver for op...