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1
artículo
Publicado 2017
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In 2013, clinically suggestive cases of PEDv were reported in pig farms in Lima and Arequipa, reaching up to 100% mortality in piglets and negative results to other viral agents such as classical swine fever (CPPv) and transmissible gastroenteritis (TGEv). For this reason, the aim of this study was to isolate and molecularly detect PEDv strains in Lima pig farms. A total of 37 stool samples and intestinal contents of piglets between 2 and 21 days of age with clinical signs suggestive of PEDv from pig farms of the department of Lima, Peru were collected. Results showed that 94.3% (35/37) were positive by the immunochromatography (IHC) test; 97.1% (34/35) of them were confirmed by RT-PCR real-time that amplified a 101-bp segment of the ORF3 gene of the PEDv. The control and positive samples showed a threshold cycle (Ct) between 10 and 21 cycles with a melting temperature (Tm) of 77.7 °C. ...
2
artículo
Publicado 2020
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The application of plant growth promoting rhizobacteria (PGPR) constitutes an ecological practice that contributes to agricultural production systems and is presented as an option for reducing the use of chemical fertilizers. The tomato is in high demand around the world, its production requires efficient nutrition to supply the quantity and quality demand that the market requires, therefore, in the present investigation two bacterial strains were evaluated: Bacillus licheniformis (IB10) and Bacillus megaterium (CT11) as growth promoters in kidney tomato seedlings by testing in seedbeds. Statistical differences are evident in the different variables evaluated, the application of specific B. licheniformis at the time of planting caused an increase of 10% in the thickness of the stem and a 100% increase in the amount of dry biomass, the weekly application of B. megaterium problems the phot...
3
artículo
Publicado 2020
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The application of plant growth promoting rhizobacteria (PGPR) constitutes an ecological practice that contributes to agricultural production systems and is presented as an option for reducing the use of chemical fertilizers. The tomato is in high demand around the world, its production requires efficient nutrition to supply the quantity and quality demand that the market requires, therefore, in the present investigation two bacterial strains were evaluated: Bacillus licheniformis (IB10) and Bacillus megaterium (CT11) as growth promoters in kidney tomato seedlings by testing in seedbeds. Statistical differences are evident in the different variables evaluated, the application of specific B. licheniformis at the time of planting caused an increase of 10% in the thickness of the stem and a 100% increase in the amount of dry biomass, the weekly application of B. megaterium problems the phot...
4
artículo
Publicado 2014
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The investigated effluents have a high BOD (Biochemical Oxygen Demand) represented mainly by proteins and lipids ; which motivated the search for microorganisms with proteolytic and lipolytic capabilities for a potential biological treatment of these effluents. 39 strains were selected for testing, of these, 41% were positive for proteolytic activity, lipolytic activity to 51% and 38% for both activities; also grew 100% in pH 9 and 10, 79.5% in pH 11 and 54% at pH 12 is worth noting that of all strains tested two (N3D and N4E) presented the best proteolytic and lipolytic activities respectively. We conclude that in tannery effluents are microorganisms with high enzymatic potential of bioconversion.
5
artículo
Publicado 2013
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The use of quinolones for the treatment of urinary tract infections (UTI) has increased dramatically, a fact that has led to the emergence of resistant strains of pathogen. The aim of the study was to determine the profiles and molecular mechanisms of quinolone-resistant uropathogenic Escherichia coli strains. For this, 3 sensitive strains of E. coli uropathogenic and 58 quinolone-resistant strains were used in the antimicrobial sensitivity with the disk diffusion method for nalidixic acid (W), ciprofloxacin (Cip), levofloxacin (Lvx), norfloxacin (Nfx) and moxifloxacin (Mxf). For molecular tests were used polymerase chain reaction (PCR), restriction fragments length polymorphism (RFLP) and sequencing of gyr A gene. Excel, Blastn and ClustalX programs were used for data analysis. Uropathogenic E. coli strains showed two major patterns: 25,90% resisted W and 43,10% resisted WCipNorLvxMxf. ...
6
artículo
Publicado 2013
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The use of quinolones for the treatment of urinary tract infections (UTI) has increased dramatically, a fact that has led to the emergence of resistant strains of pathogen. The aim of the study was to determine the profiles and molecular mechanisms of quinolone-resistant uropathogenic Escherichia coli strains. For this, 3 sensitive strains of E. coli uropathogenic and 58 quinolone-resistant strains were used in the antimicrobial sensitivity with the disk diffusion method for nalidixic acid (W), ciprofloxacin (Cip), levofloxacin (Lvx), norfloxacin (Nfx) and moxifloxacin (Mxf). For molecular tests were used polymerase chain reaction (PCR), restriction fragments length polymorphism (RFLP) and sequencing of gyr A gene. Excel, Blastn and ClustalX programs were used for data analysis. Uropathogenic E. coli strains showed two major patterns: 25,90% resisted W and 43,10% resisted WCipNorLvxMxf. ...
7
artículo
Publicado 2023
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The aim of this study was to evaluate the protective capacity of an attenuated strain of Salmonella Typhimurium as a vaccine candidate against an experimental challenge. For this purpose, 51 recently weaned female guinea pigs from a commercial farm were used and distributed into three groups of 17 animals each: one group was inoculated with the attenuated strain and challenged, the second group was challenged only, and the third group served as a control (without attenuated strain and without challenge). The animals, according to their respective groups, were immunized after two weeks of adaptation to the new rearing conditions. Five weeks after immunization they were challenged with a virulent strain of S. Typhimurium. The health status was monitored for five weeks post-challenge and fecal samples were collected to demonstrate the excretion of IgA and S. Typhimurium. Additionally, necro...
8
artículo
Objective: To evaluate the in vitro antimycotic effect of Origanum vulgare essential oil on Candida albi-cans strain ATCC 10231 Material and method: Selection of four different oregano geotypes was carried out, the essential oils were obtained by distillation by Drag with steam; Its quantitative chemical com-position was determined by Gas Chromatography, Mass Spectrometry (GC / MS). The antimicrotic activity of essential oils was evaluated in vitro through sensitivity tests with the disk diffusion method in agar culture against C. albicans. Results: The results showed high antimycotic activity of all oils from the concentration of 12.5%. Conclusions: Essential oils of Origanum vulgare from different geotypes show differences in their antifungal effects. The essential oil of Origanum vulgare may be is a pharmacological alternative for the treatment of fungal infections in the mouth.
9
artículo
Publicado 1998
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Marine bacteria have been frequently included in the scientific literature as producers substance antibacterial properties. In this study, we investigate the potential activity antibiotic in vitro of strains the marine bacterioneuston isolated from rocky litoral pools in Montemar Beach, Bay of Valparaiso, Chile. Were isolated 71 strains antagonic to Staphylococcus aureus ATCC 11632. Twentyfive strains producing strongly inhibitory substance were tested against 15 know ichthiopathogenic bacteria. Applying a classic method for marine bacteria, they were carried out phenotypic characterization of bacterioneuston strains accordingly to identified. Results showed that all fifteen ichthiopathogens strains were inhibeted by the neustonic isolates, being V anguillarum NCMB 2133, V ordalii 84/2559 and V tubiashii EX1 the most sensitive. Strains of bacterioneuston were identified as Vibrio spp. (2...
10
artículo
Publicado 1998
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Marine bacteria have been frequently included in the scientific literature as producers substance antibacterial properties. In this study, we investigate the potential activity antibiotic in vitro of strains the marine bacterioneuston isolated from rocky litoral pools in Montemar Beach, Bay of Valparaiso, Chile. Were isolated 71 strains antagonic to Staphylococcus aureus ATCC 11632. Twentyfive strains producing strongly inhibitory substance were tested against 15 know ichthiopathogenic bacteria. Applying a classic method for marine bacteria, they were carried out phenotypic characterization of bacterioneuston strains accordingly to identified. Results showed that all fifteen ichthiopathogens strains were inhibeted by the neustonic isolates, being V anguillarum NCMB 2133, V ordalii 84/2559 and V tubiashii EX1 the most sensitive. Strains of bacterioneuston were identified as Vibrio spp. (2...
11
artículo
For an implant-supported restoration of the maxillary anterior area; the buccal bone plate should have a minimum thickness to ensure an optimal and predictable behavior of soft tissue. Objective: To evaluate the mechanical behavior of stress and microstrain (von Mises yield criterion); in different thicknesses of buccal maxillary bone plates. Methods: The finite element method was used for modeling the maxillary anterior area with three different buccal bone thickness 0.5, 1 and 1.5 mm. An implant-supported restoration was modeled as well, to which a static load of 200 N was applied. Stress and microstrains were obtained in the buccal bone plates at 2, 4 and 6 mm from the alveolar crest towards the apical direction, both in cancellous and cortical bone. Results: Based on the finite element method, it was found that in the thickness of 0.5 mm of buccal bone plate (cortical) with 2 mms hei...
12
artículo
Publicado 2023
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The aim of this study was to verify if rats of the Holtzman strain can be infected with the enzootic bovine leukosis virus (VLB) when they receive blood and colostrum inocula from bovines infected by the virus via orally and intraperitoneally. This is to find new species of animals that can be used in bioassays and that provide management facilities compared to the ovine species. For this, 28 rats of the Holtzman strain were used plus 8 crossbred sheep that were used as a control group. The 28 rats were distributed into three experimental groups: i) 8 rats inoculated with blood intraperitoneally, ii) 10 rats inoculated with blood orally, and iii) 10 rats inoculated with colostrum orally. The control group of sheep was inoculated with blood intraperitoneally. The inoculum was prepared from blood and colostrum samples from a cow diagnosed as serologically positive for VLB and with persiste...
13
artículo
Publicado 2012
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Introduction: The healthy carrier is the main disseminator of pneumococcal infections through nasopharynx; an alternative for treatment is a medicinal plant. Objectives: To determine the effectivity of ten medicinal plants against Streptococcus pneumoniae. Design: In vitro experimental study. Biologic materials: Medicinal plant extracts and Streptococcus pneumoniae strains. Interventions: Ten medicinal plant extracts were put in contact in vitro with 10 different Streptococcus pneumonia strains. Main outcome measures: Medicinal plant extracts activity against Streptococcus pneumoniae strains. Results: Only almendro y Bellaco caspi showed small inhibition halos with some strains; with almendro two of 10 Pneumococcus strains were inhibited and with Bellaco caspi seven strains were inhibited and three were resistant. These results had no relation either with Pneumococcus serotype or with an...
14
artículo
Publicado 2025
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In recent years, Peru has faced a political crisis marked by continuous clashes between the Executive and Legislative branches, particularly during the 2016-2020 period. These conflicts led to various institutional responses—such as impeachments, dissolutions, and resignations—that, rather than resolving the crisis, contributed to its prolongation. This cycle of instability culminated in a wave of social unrest, expressed through mass protests and the resignation of then-president Manuel Merino. This study argues that the weakness of Peru’s presidential model, reflected in its institutional design and its limited capacity to manage presidential crises, facilitated the persistence of the Executive-Legislative conflict during the 2016-2020 period. Through the process tracing method, this research reconstructs key events and analyzes information from primary and secondary sources to u...
15
artículo
Publicado 2020
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Environmental contamination by heavy metals represents a serious problem for humans and the ecosystem due to its degree of toxicity, which is why it is necessary to have treatment alternatives for its disposal. The bioremediation process of the lead (II) and cadmium (II) metals was carried out; through the use of native fungi isolated from the oxidation pool of the metallurgical laboratory of the National University of San Agustín, Arequipa - Peru; in Sabouraud dextrose and potato dextrose broth culture media, the fungal strains were cultivated to carry out the lead (II) and cadmium (II) removal tests, a batch system was used and the removal percentage was determined. Rhizopus sp and Aspergillus niger were isolated and identified; in the removal tests it was determined that Rhizopus sp was the strain that most removed the lead (II) in 86.39 %, cadmium (II) in 69.23 % and the mixture in ...
16
artículo
Publicado 2020
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Environmental contamination by heavy metals represents a serious problem for humans and the ecosystem due to its degree of toxicity, which is why it is necessary to have treatment alternatives for its disposal. The bioremediation process of the lead (II) and cadmium (II) metals was carried out; through the use of native fungi isolated from the oxidation pool of the metallurgical laboratory of the National University of San Agustín, Arequipa -Peru; in Sabouraud dextrose and potato dextrose broth culture media, the fungal strains were cultivated to carry out the lead (II) and cadmium (II) removal tests, a batch system was used and the removal percentage was determined. Rhizopus spand Aspergillus nigerwere isolated and identified; in the removal tests it was determined that Rhizopus spwas the strain that most removed the lead (II) in 86.39 %, cadmium (II) in 69.23 % and the mixture in 74.8...
17
artículo
Publicado 2009
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En la clásica descripción de los Staphylococcus, estos son gram positivos, catalasas positivos; se presentan como cocos en racimo y son fermentadores de glucosa. La producción de coagulasa y la fermentación de manitol son las características mínimas para diferenciar S. aureus de otros Staphylococcus (1). Staphylococcus aureus expresa varios factores de virulencia, incluyendo la catalasa, que es considerada una característica de género en su identificación (2,3), excepto especies anaeróbicas de Staphylococcus saccharolyticus y S. aureus subes anaerobius (4). Sin embargo, algunos aislamientos de S. aureus catalasa negativo han sido comunicaados en humanos, desde 1955, en muestras de sangre, catéter, ulceras, aspirado traqueal, brotes intrahospitalarios, entre otros (5-8). A través de la presente carta, deseamos informar a la comunidad científica nacional e internacional acerca...
18
artículo
Publicado 2019
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The aim of this study was to determine the percentage of sensitivity or resistance of strains of E. coli isolated from asymptomatic broiler chicken against 18 common antibiotics used in poultry and in human medicine. In addition, strains that produce extended-spectrum beta-lactamases (ESBL) from a farm or slaughterhouses in the poultry area of Santander, Colombia. The circulation of strains resistant to beta-lactams, cephalosporins, aminoglycosides, quinolones and sulfonamides was determined, as well as antibiotics widely used in other non-farm environments. The content of the bursa of Fabricius was cultivated in McConkey agar obtaining 46 strains of E. coli. Susceptibility tests (n=18) were conducted to the isolated strains, and 91% of the strains were resistant to ampicillin and 80% to cephalosporins. The association of antibiotics with beta-lactamase inhibitors (ampicillin sulbactam a...
19
artículo
Publicado 2015
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The mechanisms of conversion to elemental sulfur, sulphites, sulfates and others; they are diverse, leading to the formation of sulfuric acid and therefore, to the acidification of the medium. Based on these characteristics, acidophilic bacteria are considered as sulfur chemolytotrophs, since they obtain their energy from the conversion of sulfur compounds and, in most cases, the final product is sulfate. The acid formed by these bacteria that oxidize sulfides is sulfuric acid. Similarly, bacteria isolated from acid drains, during their metabolic action, generate a decrease in pH. The Thiobacillus genus is relevant among the others for its ability to oxidize sulfur compounds, producing elemental sulfur extracellularly. The energy required to carry out its functions is derived from the oxidation of one or more reduced sulfur compounds including sulfide and thiosulfate. In the study of the...
20
artículo
Publicado 2015
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The mechanisms of conversion to elemental sulfur, sulphites, sulfates and others; they are diverse, leading to the formation of sulfuric acid and therefore, to the acidification of the medium. Based on these characteristics, acidophilic bacteria are considered as sulfur chemolytotrophs, since they obtain their energy from the conversion of sulfur compounds and, in most cases, the final product is sulfate. The acid formed by these bacteria that oxidize sulfides is sulfuric acid. Similarly, bacteria isolated from acid drains, during their metabolic action, generate a decrease in pH. The Thiobacillus genus is relevant among the others for its ability to oxidize sulfur compounds, producing elemental sulfur extracellularly. The energy required to carry out its functions is derived from the oxidation of one or more reduced sulfur compounds including sulfide and thiosulfate. In the study of the...
