The use of quinolones for the treatment of urinary tract infections (UTI) has increased dramatically, a fact that has led to the emergence of resistant strains of pathogen. The aim of the study was to determine the profiles and molecular mechanisms of quinolone-resistant uropathogenic Escherichia coli strains. For this, 3 sensitive strains of E. coli uropathogenic and 58 quinolone-resistant strains were used in the antimicrobial sensitivity with the disk diffusion method for nalidixic acid (W), ciprofloxacin (Cip), levofloxacin (Lvx), norfloxacin (Nfx) and moxifloxacin (Mxf). For molecular tests were used polymerase chain reaction (PCR), restriction fragments length polymorphism (RFLP) and sequencing of gyr A gene. Excel, Blastn and ClustalX programs were used for data analysis. Uropathogenic E. coli strains showed two major patterns: 25,90% resisted W and 43,10% resisted WCipNorLvxMxf. ...

The use of quinolones for the treatment of urinary tract infections (UTI) has increased dramatically, a fact that has led to the emergence of resistant strains of pathogen. The aim of the study was to determine the profiles and molecular mechanisms of quinolone-resistant uropathogenic Escherichia coli strains. For this, 3 sensitive strains of E. coli uropathogenic and 58 quinolone-resistant strains were used in the antimicrobial sensitivity with the disk diffusion method for nalidixic acid (W), ciprofloxacin (Cip), levofloxacin (Lvx), norfloxacin (Nfx) and moxifloxacin (Mxf). For molecular tests were used polymerase chain reaction (PCR), restriction fragments length polymorphism (RFLP) and sequencing of gyr A gene. Excel, Blastn and ClustalX programs were used for data analysis. Uropathogenic E. coli strains showed two major patterns: 25,90% resisted W and 43,10% resisted WCipNorLvxMxf. ...