1
artículo
Industrial effluents biological treatment has obtained great important, becoming essent ial in the management of effluents, but for this, requires trained microorganisms adapted to the physicochemical condit ions of the effluent, so it is necessary to characterize effluents previously to design the t ype and treatment condit ions. Tanner y effluents of the plant, where the research was conducted, have pH within alkaline range and high BOD (Biochemical Oxygen Demand) indicating abundance of organic matter. These features led to the search for microorganisms tolerant to alkaline conditions for a potential treatment of these effluents. Culture media were designed based condit ioners tannery effluents at different pH values: 9, 10, 11 and 12.Of the 39 isolates, 100% grew in pH 9 and 10; 79.5% at pH 11; and 77% at pH 12. It should be noted that from the total strains tested, two (N3D and...
2
artículo
Publicado 2010
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There is a growing concern in environmental pollution levels of the neighboring ecosystems fruit processing plant by the discharge of untreated industrial effl uents and proper control of the authorities, because many of them have not considered the management their effl uents into the design of the plant. The objective of this research is to characterize and manage the processing effl uent. We conclude that the processing effl uents have a high potential for contamination by the abundant organic load and that have achieved the primary treatment of this potential reduction to acceptable limits by Peruvian law (98.1% turbidity removal and reduction of BOD Oxygen 96.58%), allowing the reduction of water consumption, release of hardship and its incorporation as a business friendly environment for approval of environmental authorities and the community as a whole.
3
artículo
Publicado 2014
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The investigated effluents have a high BOD (Biochemical Oxygen Demand) represented mainly by proteins and lipids ; which motivated the search for microorganisms with proteolytic and lipolytic capabilities for a potential biological treatment of these effluents. 39 strains were selected for testing, of these, 41% were positive for proteolytic activity, lipolytic activity to 51% and 38% for both activities; also grew 100% in pH 9 and 10, 79.5% in pH 11 and 54% at pH 12 is worth noting that of all strains tested two (N3D and N4E) presented the best proteolytic and lipolytic activities respectively. We conclude that in tannery effluents are microorganisms with high enzymatic potential of bioconversion.
4
artículo
Cellulase production by cell immobilization for the treatment of industrial effluents lignocellulose
Publicado 2010
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Industrial effluent treatment requires highly polluting alternatives designed to lower costs and greater feasibility for adoption by the respective industrial sector. Enzymatic bioconversion of lignocellulosic waste constitutes a promising alternative for the decontamination of such effluents on behalf of our ecosystems. The immobilization of microorganisms in industrial biotechnological production of enzymes is an increase in production, ease of unit operations and reduced costs. The search for new culture media for biotechnology products desired as cellulase is also vital to raise the level of production of these compounds. The objectives were immobilized spores and assessing the production of cellulases in different culture media based on sugarcane bagasse. Agar was used to immobilize the 2, 4 and 6% as carriers. The addition of the microorganism was carried out in the form of spores ...
5
artículo
Cellulase production by cell immobilization for the treatment of industrial effluents lignocellulose
Publicado 2010
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Industrial effluent treatment requires highly polluting alternatives designed to lower costs and greater feasibility for adoption by the respective industrial sector. Enzymatic bioconversion of lignocellulosic waste constitutes a promising alternative for the decontamination of such effluents on behalf of our ecosystems. The immobilization of microorganisms in industrial biotechnological production of enzymes is an increase in production, ease of unit operations and reduced costs. The search for new culture media for biotechnology products desired as cellulase is also vital to raise the level of production of these compounds. The objectives were immobilized spores and assessing the production of cellulases in different culture media based on sugarcane bagasse. Agar was used to immobilize the 2, 4 and 6% as carriers. The addition of the microorganism was carried out in the form of spores ...
6
artículo
Publicado 2013
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El tratamiento biológico de efluentes industriales ha cobrado tanta importancia que se ha hecho imprescindible en la gestión de efluentes (Rodríguez, 2012); sin embargo requiere de microorganismos entrenados y adaptados a las condiciones fisicoquímicas del efluente, por ello es necesario caracterizar previamente los efluentes para diseñar el tipo y las condiciones de tratamiento. Los efluentes de curtiembre de la planta donde se realizó la invest igación poseen un pH variable dentro del rango alcalino y una elevada DBO que indica abundancia de materia orgánica. Estas características motivaron la búsqueda de microorganismos tolerantes a condiciones alcalinas para un potencial tratamiento de dichos efluentes. Se diseñaron medios de cult ivo tomando como base los efluentes de curtiembre acondicionados a diferentes pH: 9, 10, 11 y 12.De las 50 cepas, se seleccionaron...
7
artículo
Publicado 2010
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There is a growing concern in environmental pollution levels of the neighboring ecosystems fruit processing plant by the discharge of untreated industrial effl uents and proper control of the authorities, because many of them have not considered the management their effl uents into the design of the plant. The objective of this research is to characterize and manage the processing effl uent. We conclude that the processing effl uents have a high potential for contamination by the abundant organic load and that have achieved the primary treatment of this potential reduction to acceptable limits by Peruvian law (98.1% turbidity removal and reduction of BOD Oxygen 96.58%), allowing the reduction of water consumption, release of hardship and its incorporation as a business friendly environment for approval of environmental authorities and the community as a whole.
8
artículo
Publicado 2014
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Los efluentes investigados poseen una elevada DBO (Demanda Bioquímica de Oxígeno) representada principalmente por proteínas y lípidos; lo cual motivó la búsqueda de microorganismos con capacidades proteolíticas y lipolíticas, para un potencial tratamiento biológico de dichos efluentes. Se seleccionaron 39 cepas para los ensayos, de estas, el 41% resultaron positivas para actividad proteolítica, 51% para actividad lipolítica y 38% para ambas actividades; asimismo el 100% crecieron en pH 9 y 10, el 79.5% en pH 11 y el 54% en pH 12. Cabe resaltar que del total de cepas ensayadas dos de ellas (N3D y N4E) presentaron las mejores actividades proteolíticas y lipolíticas respectivamente. Se concluye que en los efluentes de curtiembre existen microorganismos con un alto potencial enzimático de bioconversión.
9
tesis doctoral
Publicado 2023
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Desarrolla una nueva alternativa de tratamiento biológico por bioconversión para reducir el potencial contaminante de los efluentes y revalorizar los residuos generados por la curtiembre “Unión”. Para lo cual, elige el efluente de la etapa de pelambre, fracción del efluente con mayor carga de materia orgánica, antes de que se una y se acompleje con los otros efluentes. De los efluentes característicamente alcalinos se realiza el aislamiento de microorganismos tolerantes a pH alcalinos y la capacidad de hidrolizar proteínas y lípidos. Luego de un proceso de coagulación y sedimentación de los efluentes, el sobrenadante es suplementado para mejorar el crecimiento de la cepa seleccionada (N4E) y permitir la bioconversión de la materia orgánica en biomasa celular y así reducir la cantidad de DBO5 y su potencial contaminante. Se llega a determinar a escala de laboratorio que e...
10
tesis de grado
Publicado 2010
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El desarrollo de las industrias alimentarias en el Perú ha mostrado un significativo incremento en los últimos años, tal como lo demuestra los estudios realizados por el ministerio correspondiente; sin embargo el mismo estudio revela que el mayor incremento corresponde al sector informal. Estas industrias no consideran dentro del proceso de producción la gestión de sus efluentes, que poseen alta carga orgánica con un enorme potencial contaminante, porque consideran un “gasto innecesario” que mermaría sus ganancias. Por lo cual el objetivo de la presente investigación fue desarrollar un procedimiento que permita tratar los efluentes de dos plantas procesadoras de frutas, mediante bioconversión a proteínas unicelulares. Las características promedio de los efluentes como la carga orgánica fue calculada por la DBO5 en 4,634.25 mg/L y una turbidez de 171.85 NTU. El tratamiento...
11
artículo
Publicado 2022
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Phosphorus is one of the macronutrients for the growth of vegetable crops, its low availability in the soil reduces the growth and yield of coffee crops, although the use of phosphorus fertilizers supplies the problem in the short term, in the long term they increase production costs and have a significant impact on the environment. The objective of this study was to evaluate and select microorganisms capable of solubilizing phosphate from the rhizosphere in coffee plantations (Rioja) to obtain a bank of microorganisms with biological fertilizer potential. A screening process of bacterial isolates, 15 codified bacteria from the rhizosphere of Coffea americana cultures, was carried out in SMRS1, NBRIP and Pikovskaya media to evaluate semi-quantitative tests of phosphate solubilization, having as main component tricalcium phosphate and phosphate monocalcium, obtaining values of Solubilizat...
12
artículo
Publicado 2023
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Se analizó la composición química, actividad antioxidante, antimicrobiana y antifúngica del aceite esencial de hojas y flores de Porophyllum ruderale (Jacq.) Cass. subsp. macrocephalum (DC.) R.R. Johnson originaria de las provincias de Jaén y San Ignacio en Cajamarca. Los compuestos químicos de mayor concentración detectados mediante un análisis de Cromatografía de gases acoplada a espectrometría de masas (GC-MS) en el aceite esencial fueron D-Limoneno (62,85%), 1-Undeceno (10,20%) y Cariofileno (5,08%). Se realizaron los métodos de actividad antioxidante por DPPH•, ABTS•+ y FRAP; mientras que la actividad antimicrobiana y antifúngica fueron realizados por el método por difusión en agar. Se obtuvo un TEAC de 3,0951 mg Trolox®/ g muestra por el método del radical libre DPPH•, un TEAC de 2,6942 mg Trolox®/ g muestra por el método del radical catiónico ABTS•+ y un...
13
artículo
Publicado 2023
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Petroselinum crispum (Mill) Fuss is an aromatic herb that contains various phytochemical compounds in its aerial parts, among which polyphenols stand out; likewise, it is attributed antioxidant property. The objective was to evaluate the content of phenolic compounds, the antioxidant activity of the hydroalcoholic extract of Petroselinum crispum (Mill) Fuss and the preparation of a dermocosmetic cream. The determination of polyphenols was carried out applying the in vitro method of Folin Ciocalteu and; the uptake of free radicals, by the DPPH• and ABTS•+ methods. The hydroalcoholic extract obtained a yield of 3.94 % and through a phytochemical screening the presence of phenolic compounds, flavonoids, terpenes, lactones and anthraquinones was confirmed. The following results were obtained: 4.33 ± 0.0162 mg EAG/g of phenolic compounds and TEAC-DPPH• and ABTS•+ values of 6.4322 mg ...
14
artículo
Publicado 2024
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Introduction. - The "nogal peruano" is a large tree distributed in several regions of Peru, mainly in areas of late secondary and mature forests. Species supplying antioxidant essential oils are considered valuable for health applications. Essential oils with various constituents show synergistic effects and can be an effective alternative to prevent diseases.Objectives. - To evaluate the chemical composition, antioxidant and anti-Candida albicans activity of the essential oil of Juglans neotropica Diels leaves.Materials and methods. - The essential oil was obtained by steam distillation. Its chemical composition was analyzed by GC-MS analysis and its antioxidant activity was evaluated according to DPPH• and ABTS•+ methods. In addition, its anti-Candida albicans activity was determined, adapting protocols, using ketoconazole as a reference. The oil was diluted in DMSO and incubated w...