Author contribution: All authors made an equal contribution to the development and planning of the study. Conflict of Interest: The authors have no potential conflicts of interest, or such divergences linked with this research study. Data Availability Statement: Data are available from the authors upon request.

El cacao se encuentra entre los diez productos agrícolas más importantes del mundo. El presente estudio decodificó el genoma plastidial de Cacao Fino de Aroma de Amazonas utilizando técnicas de secuenciamiento masivo. En este estudio se obtuvieron dos genomas plastidiales completos de cada una de las muestras INDES34 e INDES67, mientras que los genomas mitocondriales fueron ensamblados parcialmente (~20%). La presencia de más de un genoma plastidial por cada ejemplar de cacao, confirmaría el primer reporte de “heteroplasmia” en plantas de cacao. El tamaño de los 4 genomas plastidiales varía desde 160,589 pb hasta 160,620 pb y contienen el mismo porcentaje de GC (36.9). Están compuestos por 130 genes (37 tRNA, 8 rRNA y 85 genes codificantes de proteínas), de los cuales 17 genes están duplicados en la IR (6 genes codificantes de proteínas, 4 rRNA y 7 tRNA). Los análisis fi...

In this research, the papain enzyme was obtained from the latex of three species of the Vasconcellea genus, V. chachapoyensis, V. heilbornii and V. pubescens, species native to the Amazon region in Peru. The extracted latex was conditioned with sodium acetate buffer, EDTA and sodium bisulfite solution. The purification was carried out adapting the method of Andrade (2011), ammonium sulfate was used for precipitation; 96° ethanol in two stages for dilution; the pH was regulated at 6.0 and 8.0 with NaOH 0.1 N, it was centrifuged in three stages; frozen at -65 °C for 12 hours; and lyophilized from 24 to 36 hours with a vacuum of 0.1 to 0.6 mbar and a collector temperature of -84 to -85 ° C, finally it was packed in dark bottles. Using the milk coagulation method (Balls and Hoover) the enzymatic activity (AE) was determined, the three species obtained higher values of AE at pH 6.0 than at...

In this research, the papain enzyme was obtained from the latex of three species of the Vasconcellea genus, V. chachapoyensis, V. heilbornii and V. pubescens, species native to the Amazon region in Peru. The extracted latex was conditioned with sodium acetate buffer, EDTA and sodium bisulfite solution. The purification was carried out adapting the method of Andrade (2011), ammonium sulfate was used for precipitation; 96° ethanol in two stages for dilution; the pH was regulated at 6.0 and 8.0 with NaOH 0.1 N, it was centrifuged in three stages; frozen at -65 °C for 12 hours; and lyophilized from 24 to 36 hours with a vacuum of 0.1 to 0.6 mbar and a collector temperature of -84 to -85 ° C, finally it was packed in dark bottles. Using the milk coagulation method (Balls and Hoover) the enzymatic activity (AE) was determined, the three species obtained higher values of AE at pH 6.0 than at...

Rice (Oryza sativa L.) is a staple crop for sustaining global food security and is particularly important in tropical and subtropical regions. In this context, precision agriculture enables more efficient crop management to increase productivity and sustainability. This study proposes an integrated framework for monitoring the phenological development and estimating the yield of O. sativa by combining agronomic variables, vegetation indices (VIs), and meteorological data. Six rice varieties (Victoria, Esperanza, Bellavista, Puntilla, Capoteña, and Valor) were evaluated across six phenological stages using field data, 20 VIs and meteorological parameters. Field data revealed greater tillering of the Puntilla and Valor varieties (9–28 tillers), with Esperanza having the most stable chlorophyll values (21.5–38.7, σ = 10.46) during ripening. The temporal dynamics of the VIs consistentl...

La falta de información sobre la distribución geográfica de los sistemas de cacao, junto con la diversidad de especies y la influencia de los factores climáticos en los rendimientos, representa desafíos para la gestión agronómica de estas plantaciones y la implementación de políticas agrícolas más efectivas. Este estudio tuvo como objetivo mapear el área de cacao, la diversidad de especies y su respuesta a la variabilidad climática histórica en las provincias de Jaén y San Ignacio, Cajamarca, Perú. Se procesaron datos de PlanetScope y Sentinel-1 en Google Earth Engine utilizando el algoritmo de clasificación Random Forest. Se identificaron 4,338.6 ha de sistemas de monocultivo y agroforestería de cacao, logrando una precisión temática del 85% y un índice kappa de 0.81. Se determinó que Musa sp. predomina en altitudes bajas, mientras que Inga edulis mostró mayor dom...

The increasing use of plastics globally has generated serious environmental and human health problems, particularly in the agricultural sector where low-density polyethylene (LDPE) and other plastics are widely used. Due to its low recycling rate and slow degradation process, LDPE is a major source of pollution. This paper addresses the problem of plastic accumulation in agriculture, focusing on LDPE biodegradation strategies. The studies reviewed include recent data and the methodologies used include state-of-the-art technologies and others that have been used for decades, to monitor and measure the degree of biodegradation that each treatment applied can have, including SEM, GCMS, HPLC, and microscopy. The countries investigating these biodegradation methodologies are identified, and while some countries have been developing them for some years, others have only begun to address this p...

Soil is a vital nonrenewable resource characterized by rapid degradation and slow regeneration processes. In this study, soil degradation in Jaén and San Ignacio was assessed via a multicriteria evaluation approach combined with remote sensing (RS) data. Nine factors were analyzed classified three categories: environmental, topographic, and edaphological factors. The results revealed that the slope (59.07%) was the main influencing factor, followed by land use and land cover (LULC) (56.36%). The degradation map revealed that 83.48% of the area exhibited moderate degradation, 14.49% low degradation, and 1.56% high degradation. The districts of Pomahuaca and San José de Lourdes demonstrated the largest areas of moderate degradation, accounting for 13.71% and 22.54%, respectively. Bellavista and Huarango exhibited the largest areas of very high degradation, accounting for 0.27% and 0.08%,...

The presence of heavy metals in agricultural soils poses a threat to the development of sustainable agriculture and ensuring food security. The objective of this study was to evaluate the geospatial distribution of heavy metals in rice-growing soils within the Amojú River Basin, Jaen, Peru. Ninety-five soil samples were collected randomly, covering four altitudinal ranges, namely, very low (374–450 m above sea level (m a.s.l.)), low (450–571 m a.s.l.), medium (571–701 m a.s.l.) and high altitudes (701–1,034 m a.s.l.), and different agronomic management stages, including four phenological phases (seedling, tillering, filling and ripening) and two agricultural practices (resting and stubble burning). The evaluation was conducted via physicochemical analysis, the single-factor pollution index, and spatial interpolation techniques through the empirical Bayesian kriging (EBK) method....

The thread blight disease (TBD) of cacao (Theobroma cacao) in the department of Amazonas, Peru was recently reported to be caused by Marasmius tenuissimus (Sect. Neosessiles). This same species is known to be the main causal agent of TBD in West Africa. However, some morphological characteristics, such as the presence of rhizomorphs, the almost exclusively white color, and pileus sizes less than 5 mm, among others, differ to the description of M. tenuissimus. Therefore, we aimed to conduct a taxonomic revision of the cacao-TBD causal agent in Peru, by using thorough micro and macro morphological, phylogenetic, and nuclear and mitochondrial genomic approaches. We showed that the causal agent of TBD of cacao in Amazonas, Peru, belongs to a new species, Marasmius infestans sp. nov. This study enriches our knowledge of species in the Sect. Neosessiles, and strongly suggests that the M. tenui...

The rice–fish farming system is an efficient ecological model with economic, ecological, and social benefits, reduces environmental impacts and optimizes the use of resources. The objective of the research was to explore and analyze scientific publications through a systematic review and meta-analysis related to rice–fish intercropping. A review of publications hosted in the Scopus and PubMed database from January 2000 to April 2025 was conducted. Research articles were selected, excluding review articles, com-mentaries, book chapters, and letters, and only documents published in English were analyzed. The analysis shows that the countries with the highest number of publications were China and Bangladesh, with a proportion of 48% and 24% respectively, followed by Thailand with 10% and Pakistan, Indonesia, Malaysia, and India with 5% each. The fish species used in rice–fish systems ...