Objective: to extract and determine the antiviral activity of compounds from Chondracanthus chamissoi and Chlorella peruviana against DENV-2 in Vero-76 cells. Materials and methods: carrageenan from Chondracanthus chamissoi and soluble carbohydrates from Chlorella peruviana were extracted, and toxicity tests in VERO-76 cells and antiviral activity were determined. Results: carrageenan from Chondracanthus chamissoi sporophyte and gametophyte phases were obtained, the compound was identified as k-carrageenan using infrared light. Nine sugars (ribose, xylose, arabinose, fructose, mannose, galactose, sucrose, maltose, and lactose) were identified using chromatography in the soluble carbohydrate mix from Chondracanthus chamissoi, and four sugars (glucose, sucrose, maltose, and lactose) from Chlorella peruviana were identified. Compounds from Chondracanthus chamissoi and Chlorella peruviana so...

Objective: to extract and determine the antiviral activity of compounds from Chondracanthus chamissoi and Chlorella peruviana against DENV-2 in Vero-76 cells. Materials and methods: carrageenan from Chondracanthus chamissoi and soluble carbohydrates from Chlorella peruviana were extracted, and toxicity tests in VERO-76 cells and antiviral activity were determined. Results: carrageenan from Chondracanthus chamissoi sporophyte and gametophyte phases were obtained, the compound was identified as k-carrageenan using infrared light. Nine sugars (ribose, xylose, arabinose, fructose, mannose, galactose, sucrose, maltose, and lactose) were identified using chromatography in the soluble carbohydrate mix from Chondracanthus chamissoi, and four sugars (glucose, sucrose, maltose, and lactose) from Chlorella peruviana were identified. Compounds from Chondracanthus chamissoi and Chlorella peruviana so...

The presence of Plesiomonas shigelloides causing enteric disease in tilapia Oreochromis niloticus in a fish farm in Lima region, Peru is reported. The bacteria were isolated and identified as Plesiomonas shigelloides by conventional biochemical techniques and confirmed by the API 20NE system (numerical profile 7162744 that ratifies 99.3% P. shigelloides). Strains evaluated (n=4) had a fermentative metabolism of glucose, test positive to cytochrome oxidase, indole, Voges-Proskauer and methyl red. The most frequent external signs were erythema in abdomen and pectoral fins, and prominent and reddened anus with bloody discharge. Internally, ascites in visceral cavity and gut inflammation were detected. Histopathology studies revealed edema of the lamina propia and epithelial necrosis of gastric and intestinal tissue, and necrosis in anterior kidney and gonad.

En el presente trabajo se reporta la presencia de Plesiomonas shigelloides causando un cuadro patológico entérico en tilapia Oreochromis niloticus en una piscigranja de la región Lima, Perú. Las bacterias fueron aisladas e identificadas por técnicas bioquímicas convencionales y confirmadas por el sistema API 20NE (perfil numérico 7162744 que ratifica a P. shigelloides al 99.3%). Las cepas evaluadas (n=4) presentaron un metabolismo fermentativo de glucosa, positividad en las pruebas de citocromo oxidasa, indol, Voges-Proskauer y rojo de metilo. Los signos externos más frecuentes de la enfermedad fueron eritema en el vientre y las aletas pectorales, ano prominente y enrojecido con descarga sanguinolenta. Internamente se observó el intestino inflamado y la presencia de líquido ascítico en la cavidad visceral. El estudio histopatológico reveló edema de la lámina propia y necros...

The aim of the study was to evaluate five salinity levels on the growth and productivity of Chlorella peruviana Chacon, a native strain of the Mellisera salt lagoon in Chilca, Peru. Five treatments were used according to the salinity level: T1, 38‰; T2, 44‰; T3, 59‰; T4, 88‰; and T5, 176‰, with a temperature of 27±2 °C and a light intensity of 1250±10 lx. Each treatment was done in triplicate in flasks of 250, 500 and 1000 ml. Cultures were performed with f/2 medium (Guillard), constant aeration and photoperiod of 24:00 h. The results indicated that T1 showed better results in density (42±2.65x106 cel/ml), dry biomass (397.9±2.3 mg/l) and chlorophyll (80.2±2.3 mg/l), but with adequate yields at higher levels of salinity. It is concluded that the growth and productivity of C. peruviana presents a decreasing trend in relation to the level of salinity.

El objetivo del estudio fue evaluar cinco niveles de salinidad sobre el crecimiento y productividad de Chlorella peruviana Chacon, cepa nativa de la laguna salina Mellisera, Chilca, Perú. Se utilizaron cinco tratamientos de acuerdo al nivel de salinidad: T1, 38‰; T2, 44‰; T3, 59‰; T4, 88‰; y T5, 176‰, con una temperatura de 27±2 °C y una intensidad de luz de 1250±10 lx. Cada tratamiento se realizó por triplicado en matraces de 250, 500 y 1000 ml. Los cultivos se realizaron con medio f/2 (Guillard), aireación constante y fotoperiodo de 24:00 h. Los resultados indicaron que T1 presentó mejores resultados en densidad (42±2.6x106 cel/ml), biomasa seca (397.9±2.3 mg/l) y clorofila (80.2±2.3 mg/l), pero con rendimientos adecuados en los niveles superiores de salinidad. Se concluye que el crecimiento y productividad de C. peruviana presenta una tendencia decreciente con rela...

In the present research, nineteen advanced potato clones with pigmented-fleshed were analyzed in two potato-producing localities in Cajamarca, Peru, during two agricultural seasons, by means of the combined analysis of variance, the model of additive main effects and multiplicative interaction (AMMI) and the Biplot analysis, to analyze the genotype-environment interaction (GEI), in order to select clones with high stability of marketable yield and quality. Multivariate analysis showed differences for the main effects of genotypes, environments and GEI. Which states that the genetic makeup of each clone and the environment influenced the marketable yield and frying color due to the polygenic character that governs these characteristics. The results identified the CIP clone 302299.28 with red and cream fleshed and red skin with low IGA, which indicates that it is a stable clone with high c...

In the present research, nineteen advanced potato clones with pigmented-fleshed were analyzed in two potato-producing localities in Cajamarca, Peru, during two agricultural seasons, by means of the combined analysis of variance, the model of additive main effects and multiplicative interaction (AMMI) and the Biplot analysis, to analyze the genotype-environment interaction (GEI), in order to select clones with high stability of marketable yield and quality. Multivariate analysis showed differences for the main effects of genotypes, environments and GEI. Which states that the genetic makeup of each clone and the environment influenced the marketable yield and frying color due to the polygenic character that governs these characteristics. The results identified the CIP clone 302299.28 with red and cream fleshed and red skin with low IGA, which indicates that it is a stable clone with high c...

Objective: Adapt the microneutralization test (MNT) to detect neutralizing antibodies against dengue serotype 2 virus (DENV-2) in the Vero-76 cell line.  Materials and methods. Different cellular concentrations (0.6 x 105 cell / mL, 0.9 x 105 cell / mL, 1.2 x 105 cell / mL) and percentages of fetal bovine serum (FBS) at 2%, 3% and 4%, passages number of virus stock and incubation days were evaluated. The viral seed was confirmed by RT-qPCR. DENV-2 was propagated by carrying out 5 successive passages in the Vero-76 cell line, then the virus was titrated in microplates and 2 methods were evaluated: complete monolayer and cell suspension, and the optimal coloring day was determined.  Obtained the results, it was evaluated by the MNT for DENV-2: 5 sera negative to DENV-2 and YFV, 5 sera negative to DENV-2 and seropositive to YFV and 5 seropositive sera to DENV-2 confirmed by plaque...

Objetivo: Estandarizar la prueba de microneutralización (MNT) para detección de anticuerpos neutralizantes contra el virus dengue serotipo 2 (DENV-2) en la línea celular Vero-76.  Materiales y métodos: Se evaluaron diferentes concentraciones celulares (0,6 x105 cel/mL, 0,9 x105 cel/mL, 1,2 x105 cel/mL), porcentajes de 2%, 3% y 4% de suero bovino fetal (SBF), número de pasajes del stock de virus y los días de incubación. La semilla viral se confirmó por RT-qPCR. El DENV-2 se propagó realizando 5 pasajes en células Vero-76, seguidamente se tituló el virus en placas de 96 pozos y se evaluaron 2 métodos de infección celular: monocapa y células en suspensión, además se determinó el día óptimo de coloración de las células. Obtenidos los resultados, se procesaron mediante MNT para DENV-2 las siguientes muestras: 5 sueros negativos a DENV-2 y YFV, 5 sueros negativos a D...

El presente trabajo evaluó el efecto de la inclusión dietaría de Chlorella peruviana sobre los parámetros de crecimiento y curvas de incremento de la relación longitud-peso de alevines de Colossoma macropomum. Se prepararon cuatro dietas con diferentes niveles de adición de C. peruviana (0%, 2.5%, 5% y 7.5%). Se emplearon un total de 180 alevines con longitud y peso inicial de 4,03 ± 0,62cm y 1,94 ± 0,65 gr respectivamente, fueron cultivados en doce acuarios de vidrio de 0.27 m3 de volumen a una capacidad de 15 peces por acuario y alimentados tres veces al día, durante 70 días, a una tasa de alimentación del 15% durante los primeros 50 días y de 3% en los últimos 20 días de estudio. La composición corporal de los peces fue determinado al inicio y al final del estudio. La calidad del agua fue monitoreada diariamente (oxígeno disuelto, temperatura y pH) y quincenalmente (ni...