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1
artículo
Publicado 2019
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The development rates and the quality of the bovine embryos produced in vitro are lower than those produced in vivo, due to the oxidative stress to which they are subjected during their manipulation and the culture conditions. The aim of this study was to evaluate the production, cellularity and vitality of bovine blastocysts produced in vitro with different concentrations of quercetin. A total of 2108 oocytes were matured in vitro in medium 199 with 10% FBS, 5.0 μg/ml LH and 0.5 μg/ml FSH. Fertilization was carried out in TALP medium with 2x106 spermatozoa/ml. Potential zygotes were cultured at 38.7 °C with 5% CO2 in SOF culture medium supplemented with six concentrations of quercetin (1, 5, 10, 15, 20, 50 μM) and as controls were used the SOF medium without quercetin and supplemented with DMSO. The rates of cleavage (day 2) and blastocysts (day 7) were determined, as well as the ce...
2
artículo
Publicado 2019
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The development rates and the quality of the bovine embryos produced in vitro are lower than those produced in vivo, due to the oxidative stress to which they are subjected during their manipulation and the culture conditions. The aim of this study was to evaluate the production, cellularity and vitality of bovine blastocysts produced in vitro with different concentrations of quercetin. A total of 2108 oocytes were matured in vitro in medium 199 with 10% FBS, 5.0 μg/ml LH and 0.5 μg/ml FSH. Fertilization was carried out in TALP medium with 2x106 spermatozoa/ml. Potential zygotes were cultured at 38.7 °C with 5% CO2 in SOF culture medium supplemented with six concentrations of quercetin (1, 5, 10, 15, 20, 50 μM) and as controls were used the SOF medium without quercetin and supplemented with DMSO. The rates of cleavage (day 2) and blastocysts (day 7) were determined, as well as the ce...
3
artículo
Publicado 2022
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The aim of this study was to determine the factors that affect pregnancy in recipient females of embryos produced in vitro under high altitude conditions. The study was carried out at the Universidad Nacional del Altiplano (UNA), located in Puno, Peru, at an altitude of 3970 m. 50 recipient females (nulliparous, primiparous and multiparous) Brown Swiss were used. Ovaries were collected from cows at a local slaughterhouse and taken to the UNA animal reproduction laboratory, where the oocytes were collected, and in vitro maturation and fertilization, culture and embryo evaluation were carried out. The transport time to the laboratory was 2 to 3 h. Quantitative factors such as body condition score (CC), diameter of the corpus luteum (CL) and number of parities, and qualitative factors such as the place of deposit of the embryo (right or left horn) and embryo quality, were evaluated using th...
4
artículo
Publicado 2020
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The aim of this study was to determine the influence of a co-culture system with cumulus oophorus cells on the quality of bovine embryos produced in vitro, based on their viability. Ovaries from crossbred bovine slaughtered at the Chachapoyas Municipal Benefit Centre, Amazonas, Peru, were used. Oocytes aspirated from 2-6 mm follicles were matured in TCM-199 medium for 24 hours, in a humidified atmosphere with 6% CO2 and 38.5 °C. The maturation state was determined by the nuclear stage through aceto-orcein staining. The mature oocytes were fertilized in vitro with frozen semen from Angus bulls for 18 hours. The presumed zygotes were randomly distributed for development in one group in medium with co-culture and another in medium without co-culture (control). The embryonic quality was determined based on the enzymatic activity and DNA damage of the embryos on the seventh day of cultu...
5
artículo
Publicado 2018
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The study was carried out with the in vitro embryo production of the In Vitro Colombia® laboratory of 2015. The embryo cleavage rate, the in vitro embryo production (IVP) and the pregnancy rate were analysed based on the type of semen used (sexed and conventional) for fertilization and on the bovine species (Bos taurus, Bos indicus). Data from 840 sessions of follicular aspiration were analysed, resulting in 127 503 oocytes placed in in vitro culture from 5257 females. The embryonic cleavage rate for sexed semen was 69.6% and for conventional semen 74.5% (p<0.001). It was obtained 20.8% of IVP for sexed semen and 26.5% for conventional semen (p<0.001). The sexed semen of B. taurus performed better in terms of embryonic cleavage than that of B. indicus (71.1 and 64.4%, respectively (p<0.001), whereas the conventional semen of B. indicus performed better than B. taurus (76.0 and ...
6
artículo
Publicado 2017
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The present study was carried out in a laboratory of Animal Reproduction of the Faculty of Veterinary Medicine and Animal Science of the National University of the Puno-Peru Highland. The main objective was to evaluate the effect of in vitro and in vivo culture of alpacas zygotes produced in vitro. We used 226 cumulus oocyte complexes (CCOs), which were obtained from the ovaries from animals that were beneficiated in the camal and transported in 0.9% saline and supplemented with antibiotics. CCOs were aspirated from follicles of 2 to 6 mm. The oocytes were matured in TCM 2520, supplemented with 2.2 mg/mL with sodium bicarbonate, 0.0028 mg/mL sodium pyruvate, 10% fetal serum, 2 IU/mL equine chorionic gonadotropin, 10 IU/mL chorionic gonadotropin Human plus 50 ug/mL gentamicin and were cultured at 38.5°C, under 5% CO2, and high humidity for 36 h. Fertilization was...
7
artículo
Publicado 2017
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The present study was carried out in a laboratory of Animal Reproduction of the Faculty of Veterinary Medicine and Animal Science of the National University of the Puno-Peru Highland. The main objective was to evaluate the effect of in vitro and in vivo culture of alpacas zygotes produced in vitro. We used 226 cumulus oocyte complexes (CCOs), which were obtained from the ovaries from animals that were beneficiated in the camal and transported in 0.9% saline and supplemented with antibiotics. CCOs were aspirated from follicles of 2 to 6 mm. The oocytes were matured in TCM 2520, supplemented with 2.2 mg/mL with sodium bicarbonate, 0.0028 mg/mL sodium pyruvate, 10% fetal serum, 2 IU/mL equine chorionic gonadotropin, 10 IU/mL chorionic gonadotropin Human plus 50 ug/mL gentamicin and were cultured at 38.5°C, under 5% CO2, and high humidity for 36 h. Fertilization was...
8
artículo
Publicado 2017
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The present study was carried out in a laboratory of Animal Reproduction of the Faculty of Veterinary Medicine and Animal Science of the National University of the Puno-Peru Highland. The main objective was to evaluate the effect of in vitro and in vivo culture of alpacas zygotes produced in vitro. We used 226 cumulus oocyte complexes (CCOs), which were obtained from the ovaries from animals that were beneficiated in the camal and transported in 0.9% saline and supplemented with antibiotics. CCOs were aspirated from follicles of 2 to 6 mm. The oocytes were matured in TCM 2520, supplemented with 2.2 mg/mL with sodium bicarbonate, 0.0028 mg/mL sodium pyruvate, 10% fetal serum, 2 IU/mL equine chorionic gonadotropin, 10 IU/mL chorionic gonadotropin Human plus 50 ug/mL gentamicin and were cultured at 38.5°C, under 5% CO2, and high humidity for 36 h. Fertilization was...
9
artículo
Publicado 2017
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The present study was carried out in a laboratory of Animal Reproduction of the Faculty of Veterinary Medicine and Animal Science of the National University of the Puno-Peru Highland. The main objective was to evaluate the effect of in vitro and in vivo culture of alpacas zygotes produced in vitro. We used 226 cumulus oocyte complexes (CCOs), which were obtained from the ovaries from animals that were beneficiated in the camal and transported in 0.9% saline and supplemented with antibiotics. CCOs were aspirated from follicles of 2 to 6 mm. The oocytes were matured in TCM 2520, supplemented with 2.2 mg/mL with sodium bicarbonate, 0.0028 mg/mL sodium pyruvate, 10% fetal serum, 2 IU/mL equine chorionic gonadotropin, 10 IU/mL chorionic gonadotropin Human plus 50 ug/mL gentamicin and were cultured at 38.5°C, under 5% CO2, and high humidity for 36 h. Fertilization was...
10
artículo
Effects of aqueous extract of Origanum vulgare L. (Lamiaceae) on the preimplantational mouse embryos
Publicado 2010
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The use of medicinal plants for the treatment of illnesses is widely known. However, there are not many scientific reports about the properties of these plants and their side effects. In this study, the effect of the aqueous extract of Origanum vulgare on the preimplantational mouse embryo development was investigated. The oregano aqueous extract was given ad libitum to four separated groups (n= 10) of pregnant mice: O, 9, 18 y 36 mg/mL respectively. When the embryos were evaluated, a slight delay in the embryo development was observed, but only with the highest dose. With respect to embryo quality, an increase of degenerated embryos was observed but this was not significant. These results showed that the aqueous extract of O. vulgare does not have a toxic effect on preimplantational mouse embryo, and it only produces a slight delay in embryo development.
11
artículo
Effects of aqueous extract of Origanum vulgare L. (Lamiaceae) on the preimplantational mouse embryos
Publicado 2010
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The use of medicinal plants for the treatment of illnesses is widely known. However, there are not many scientific reports about the properties of these plants and their side effects. In this study, the effect of the aqueous extract of Origanum vulgare on the preimplantational mouse embryo development was investigated. The oregano aqueous extract was given ad libitum to four separated groups (n= 10) of pregnant mice: O, 9, 18 y 36 mg/mL respectively. When the embryos were evaluated, a slight delay in the embryo development was observed, but only with the highest dose. With respect to embryo quality, an increase of degenerated embryos was observed but this was not significant. These results showed that the aqueous extract of O. vulgare does not have a toxic effect on preimplantational mouse embryo, and it only produces a slight delay in embryo development.
12
artículo
Publicado 2017
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This study demonstrates the use of nuclear somatic cell transfer to produce the first cloned cattle in Peru. Skin fibroblasts and cumulus cells from adult donors were obtained for use as carioplasts; likewise, oocytes obtained from ovaries in the slaughterhouse were matured in vitro for 24 h. The mature oocytes were incubated 2 h in demecolcin (2.5 μg/ml) to promote cone formation with the metaphase plate and to guide manual enucleation. The zona pellucida in pronase (2 mg/ml) was removed for 3 min. The enucleation was manual with a microblade dividing the ova into two halves, where the nucleus-lacking halves were fused by the «sandwich» method (cytoplast–fibroblast– cytoplast). The reconstructed structures were chemically activated by incubation for 5 min in 7% absolute ethanol, followed by 5 h of cytochalacin B (5 μg/ml) and cycloheximide (10 μg/ml). The structures were cultur...
13
artículo
Publicado 2000
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The growing interest in natural medicine makes it necessary to study plant properties as well as their possible secondary effects. In recent years the toxic effects of many medicinal plants on the preimplantational mouse embryo development have been studied. Many of them produce malformations and alterations in the embryonic development. Ruta graveolens "ruda", Origanum vulgare "oregano" and Persea americana "palta" are used in rural areas to menstrual colic and to provoke abortion (estrella, 1995). This study is aimed at assessing "in vivd'the effect of extracts of "oregano", "ruda" and "palta" to 20% on the morphology and growth of preimplantational mouse embryos.
14
artículo
Publicado 2019
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The objective was to determine the addition of 5% carbon dioxide (CO2) from effervescent granules or gasified to a portable incubator (taper) to evaluate the in vitro production of embryos of oocytes aspirated from ovaries of cows slaughtered in the slaughterhouse and evaluate viability in vivo through pregnancy in recipient cows. As a portable incubator, a 500 cm3 plastic taper was used, to which a valve for controlling the addition of CO2 was conditioned. The maturation was carried out for 24 h, fertilization for 18 h and embryo culture for 7 d in 500 uL of the media in petry plates and introduced into the portable incubator, which was placed in a water bath at 38.5 ° C. At 72 h post fertilization the proportion of zygotes was determined, at 7 d post culture the embryos were counted. The fertilization results with the addition of CO2 from effervescent granules was 38.28...
15
artículo
Publicado 2019
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The objective was to determine the addition of 5% carbon dioxide (CO2) from effervescent granules or gasified to a portable incubator (taper) to evaluate the in vitro production of embryos of oocytes aspirated from ovaries of cows slaughtered in the slaughterhouse and evaluate viability in vivo through pregnancy in recipient cows. As a portable incubator, a 500 cm3 plastic taper was used, to which a valve for controlling the addition of CO2 was conditioned. The maturation was carried out for 24 h, fertilization for 18 h and embryo culture for 7 d in 500 uL of the media in petry plates and introduced into the portable incubator, which was placed in a water bath at 38.5 ° C. At 72 h post fertilization the proportion of zygotes was determined, at 7 d post culture the embryos were counted. The fertilization results with the addition of CO2 from effervescent granules was 38.28...
16
artículo
Publicado 2019
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The objective was to determine the addition of 5% carbon dioxide (CO2) from effervescent granules or gasified to a portable incubator (taper) to evaluate the in vitro production of embryos of oocytes aspirated from ovaries of cows slaughtered in the slaughterhouse and evaluate viability in vivo through pregnancy in recipient cows. As a portable incubator, a 500 cm3 plastic taper was used, to which a valve for controlling the addition of CO2 was conditioned. The maturation was carried out for 24 h, fertilization for 18 h and embryo culture for 7 d in 500 uL of the media in petry plates and introduced into the portable incubator, which was placed in a water bath at 38.5 ° C. At 72 h post fertilization the proportion of zygotes was determined, at 7 d post culture the embryos were counted. The fertilization results with the addition of CO2 from effervescent granules was 38.28...
17
artículo
Publicado 2019
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The objective was to determine the addition of 5% carbon dioxide (CO2) from effervescent granules or gasified to a portable incubator (taper) to evaluate the in vitro production of embryos of oocytes aspirated from ovaries of cows slaughtered in the slaughterhouse and evaluate viability in vivo through pregnancy in recipient cows. As a portable incubator, a 500 cm3 plastic taper was used, to which a valve for controlling the addition of CO2 was conditioned. The maturation was carried out for 24 h, fertilization for 18 h and embryo culture for 7 d in 500 uL of the media in petry plates and introduced into the portable incubator, which was placed in a water bath at 38.5 ° C. At 72 h post fertilization the proportion of zygotes was determined, at 7 d post culture the embryos were counted. The fertilization results with the addition of CO2 from effervescent granules was 38.28...
18
artículo
Publicado 2018
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El estudio se realizó con la producción de embriones in vitro del laboratorio In Vitro Colombia® de 2015. Se analizó la tasa de división embrionaria, la producción de embriones in vitro (PIV) y la tasa de preñez teniendo en cuenta el tipo de semen utilizado (sexado y convencional) para la fertilización y la especie bovina (Bos taurus, Bos indicus). Se analizaron datos de 840 sesiones de aspiración folicular, resultando 127 503 oocitos puestos en cultivo in vitro provenientes de 5257 hembras. La tasa de división embrionaria para el semen sexado fue de 69.6% y para el semen convencional de 74.5% (p<0.001). Se obtuvo 20.8% de PIV para el semen sexado y de 26.5% para el convencional (p<0.001). El semen sexado de B. taurus se comportó mejor en términos de división embrionaria que el de B. indicus (71.1 vs. 64.4%) (p<0.001), mientras que el semen convencional de B. indicu...
19
artículo
Publicado 2021
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In the present work was evaluated the content of cadmium in soils, leaves, fresh almonds (testa and embryo), dried fermented almonds, cocoa liquor, and chocolate bars with 70% cocoa, two provinces (Bellavista and Huallaga), and 5 locations (3 in Bellavista and 2 in Huallaga) of the San Martín Region. The cadmium content was determined with an atomic absorption spectrophotometer, using a filtered sample obtained from 0.5 g of dry sample and 5 ml of perchloric nitric solution, after digestion; tests carried out in laboratories of the Universidad Nacional de San Martín (UNSM). The maximum values obtained for cadmium content (ppm) were: in soils, 0.960; in sheets 0.780; in testa and cotyledon 0.098; in fermented and dried almonds 0.075; in cocoa liquor, 0.210; in chocolate tablets with 70% cocoa 0.625. The correlation coefficient (R2) for fermented and dried almonds, indicates that there i...
20
artículo
Publicado 2022
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Introduction: Maytenus macrocarpa (MM) "Chuchuhuasi" is a native plant from the Amazon region of South America, widely used in traditional medicine due its anti-inflammatory, analgesic and anti-tumor properties. However, its effects on reproductive physiology and male fertility have not been elucidated. Methods: Preclinical experimental study of cases and controls. Male mice with eight weeks from birth were administered orally for 70 days (two spermatogenic cycles) an aqueous extract MM: GC (n = 8) 0 mg / kg body weight (bw) control group and treated group GT (n = 8) 1000 mg / kg bw. Later the animals were euthanized, the reproductive organs were weighted, also motility and sperm count were recorded. During the days 60 or 65 of treatment, males were mated and pregnant females were sacrificed in the fourth day of pregnancy for evaluating development, quality and degree of blastulati...
