El objetivo fue determinar las características ultrasonográfica B mode y Doppler del folículo dominante y cuerpo lúteo en vacas Criollas sometidas a un protocolo de sincronización de celo. El estudio fue realizado en la Estación Experimental Agraria ILLPA durante los meses de mayo a julio utilizando 15 vacas de raza Criolla con condición corporal regular y alimentadas con pasto natural, el protocolo consistió en colocar benzoato de estradiol y dispositivo intravaginal el primer día, posteriormente el día 7 se retiró el dispositivo y se aplicó un análogo de Prostaglandina F2α, el día 8 se aplicó nuevamente benzoato de estradiol y el día 9 se realizó la inseminación artificial con semen de un solo reproductor de raza Fleckvieh, la evaluación ultrasonográfica fue realizada los días 7 y 9 (iniciado la sincronización) para determinar el diámetro del folículo dominante...

El objetivo del presente estudio fue caracterizar la dinámica folicular con un protocolo de sincronización de celo con progestágenos en vacas y vaquillas Brown Swiss en el Altiplano Peruano. El estudio se realizará en el Centro de Investigación y Producción Chuquibambilla–Universidad Nacional del Altiplano de Puno, ubicado en el distrito de Umachiri, provincia de Melgar, departamento de Puno para lo cual se utilizaron 11 vacas en producción, 9 vacas en seca y 9 vaquillas de la raza Brown Swiss las cuales fueron sometidas a un protocolo de sincronización de celo con Progestágenos y eCG (Gonadotropina Coriónica Equina) y evaluadas por ultrasonografía fueron al inicio del protocolo, a los días 8 y 10 respetivamente para las características de morfometría ovárica las mismas que fueron sometidas a un diseño completamente al azar y el día 45 post inicio del protocolo para el...

The objective was to determine the addition of 5% carbon dioxide (CO2) from effervescent granules or gasified to a portable incubator (taper) to evaluate the in vitro production of embryos of oocytes aspirated from ovaries of cows slaughtered in the slaughterhouse and evaluate viability in vivo through pregnancy in recipient cows. As a portable incubator, a 500 cm3 plastic taper was used, to which a valve for controlling the addition of CO2 was conditioned. The maturation was carried out for 24 h, fertilization for 18 h and embryo culture for 7 d in 500 uL of the media in petry plates and introduced into the portable incubator, which was placed in a water bath at 38.5 ° C. At 72 h post fertilization the proportion of zygotes was determined, at 7 d post culture the embryos were counted. The fertilization results with the addition of CO2 from effervescent granules was 38.28...

The objective was to determine the addition of 5% carbon dioxide (CO2) from effervescent granules or gasified to a portable incubator (taper) to evaluate the in vitro production of embryos of oocytes aspirated from ovaries of cows slaughtered in the slaughterhouse and evaluate viability in vivo through pregnancy in recipient cows. As a portable incubator, a 500 cm3 plastic taper was used, to which a valve for controlling the addition of CO2 was conditioned. The maturation was carried out for 24 h, fertilization for 18 h and embryo culture for 7 d in 500 uL of the media in petry plates and introduced into the portable incubator, which was placed in a water bath at 38.5 ° C. At 72 h post fertilization the proportion of zygotes was determined, at 7 d post culture the embryos were counted. The fertilization results with the addition of CO2 from effervescent granules was 38.28...

The objective was to determine the addition of 5% carbon dioxide (CO2) from effervescent granules or gasified to a portable incubator (taper) to evaluate the in vitro production of embryos of oocytes aspirated from ovaries of cows slaughtered in the slaughterhouse and evaluate viability in vivo through pregnancy in recipient cows. As a portable incubator, a 500 cm3 plastic taper was used, to which a valve for controlling the addition of CO2 was conditioned. The maturation was carried out for 24 h, fertilization for 18 h and embryo culture for 7 d in 500 uL of the media in petry plates and introduced into the portable incubator, which was placed in a water bath at 38.5 ° C. At 72 h post fertilization the proportion of zygotes was determined, at 7 d post culture the embryos were counted. The fertilization results with the addition of CO2 from effervescent granules was 38.28...

The objective was to determine the addition of 5% carbon dioxide (CO2) from effervescent granules or gasified to a portable incubator (taper) to evaluate the in vitro production of embryos of oocytes aspirated from ovaries of cows slaughtered in the slaughterhouse and evaluate viability in vivo through pregnancy in recipient cows. As a portable incubator, a 500 cm3 plastic taper was used, to which a valve for controlling the addition of CO2 was conditioned. The maturation was carried out for 24 h, fertilization for 18 h and embryo culture for 7 d in 500 uL of the media in petry plates and introduced into the portable incubator, which was placed in a water bath at 38.5 ° C. At 72 h post fertilization the proportion of zygotes was determined, at 7 d post culture the embryos were counted. The fertilization results with the addition of CO2 from effervescent granules was 38.28...

The present study was carried out in a laboratory of Animal Reproduction of the Faculty of Veterinary Medicine and Animal Science of the National University of the Puno-Peru Highland. The main objective was to evaluate the effect of in vitro and in vivo culture of alpacas zygotes produced in vitro. We used 226 cumulus oocyte complexes (CCOs), which were obtained from the ovaries from animals that were beneficiated in the camal and transported in 0.9% saline and supplemented with antibiotics. CCOs were aspirated from follicles of 2 to 6 mm. The oocytes were matured in TCM 2520, supplemented with 2.2 mg/mL with sodium bicarbonate, 0.0028 mg/mL sodium pyruvate, 10% fetal serum, 2 IU/mL equine chorionic gonadotropin, 10 IU/mL chorionic gonadotropin Human plus 50 ug/mL gentamicin and were cultured at 38.5°C, under 5% CO2, and high humidity for 36 h. Fertilization was...

The present study was carried out in a laboratory of Animal Reproduction of the Faculty of Veterinary Medicine and Animal Science of the National University of the Puno-Peru Highland. The main objective was to evaluate the effect of in vitro and in vivo culture of alpacas zygotes produced in vitro. We used 226 cumulus oocyte complexes (CCOs), which were obtained from the ovaries from animals that were beneficiated in the camal and transported in 0.9% saline and supplemented with antibiotics. CCOs were aspirated from follicles of 2 to 6 mm. The oocytes were matured in TCM 2520, supplemented with 2.2 mg/mL with sodium bicarbonate, 0.0028 mg/mL sodium pyruvate, 10% fetal serum, 2 IU/mL equine chorionic gonadotropin, 10 IU/mL chorionic gonadotropin Human plus 50 ug/mL gentamicin and were cultured at 38.5°C, under 5% CO2, and high humidity for 36 h. Fertilization was...

The present study was carried out in a laboratory of Animal Reproduction of the Faculty of Veterinary Medicine and Animal Science of the National University of the Puno-Peru Highland. The main objective was to evaluate the effect of in vitro and in vivo culture of alpacas zygotes produced in vitro. We used 226 cumulus oocyte complexes (CCOs), which were obtained from the ovaries from animals that were beneficiated in the camal and transported in 0.9% saline and supplemented with antibiotics. CCOs were aspirated from follicles of 2 to 6 mm. The oocytes were matured in TCM 2520, supplemented with 2.2 mg/mL with sodium bicarbonate, 0.0028 mg/mL sodium pyruvate, 10% fetal serum, 2 IU/mL equine chorionic gonadotropin, 10 IU/mL chorionic gonadotropin Human plus 50 ug/mL gentamicin and were cultured at 38.5°C, under 5% CO2, and high humidity for 36 h. Fertilization was...

The present study was carried out in a laboratory of Animal Reproduction of the Faculty of Veterinary Medicine and Animal Science of the National University of the Puno-Peru Highland. The main objective was to evaluate the effect of in vitro and in vivo culture of alpacas zygotes produced in vitro. We used 226 cumulus oocyte complexes (CCOs), which were obtained from the ovaries from animals that were beneficiated in the camal and transported in 0.9% saline and supplemented with antibiotics. CCOs were aspirated from follicles of 2 to 6 mm. The oocytes were matured in TCM 2520, supplemented with 2.2 mg/mL with sodium bicarbonate, 0.0028 mg/mL sodium pyruvate, 10% fetal serum, 2 IU/mL equine chorionic gonadotropin, 10 IU/mL chorionic gonadotropin Human plus 50 ug/mL gentamicin and were cultured at 38.5°C, under 5% CO2, and high humidity for 36 h. Fertilization was...

El objetivo del trabajo fue evaluar el efecto de la suplementación de concentrado fibroso en la dieta sobre el desempeño reproductivo de alpacas en crianza tradicional en la Puna seca los Andes peruanos a 4090 msnm, a fin de incrementar la tasa de fertilidad y natalidad, el peso y condición corporal de las madres, y el peso al nacimiento de las crías. Se trabajó con 60 hembras de primer al tercer parto, con similares pesos (37.8 ± 1.2 kg) y condición corporal (2.31 ± 0.42), distribuidas en un grupo suplementado y un grupo sin suplementación alimenticia. El concentrado fibroso fue preparado con heno molido (12 mmø) de avena y alfalfa, afrecho de cebada, torta de soya, mezcla vitamínico-mineralÒ, sal común y melaza (EM 2.48 kcal/g y PC 12%). El consumo del concentrado se inició 10 días antes de la parición, a razón de 200 g/día/animal, continuando durante la parición, em...

The aim of this study was to determine the factors that affect pregnancy in recipient females of embryos produced in vitro under high altitude conditions. The study was carried out at the Universidad Nacional del Altiplano (UNA), located in Puno, Peru, at an altitude of 3970 m. 50 recipient females (nulliparous, primiparous and multiparous) Brown Swiss were used. Ovaries were collected from cows at a local slaughterhouse and taken to the UNA animal reproduction laboratory, where the oocytes were collected, and in vitro maturation and fertilization, culture and embryo evaluation were carried out. The transport time to the laboratory was 2 to 3 h. Quantitative factors such as body condition score (CC), diameter of the corpus luteum (CL) and number of parities, and qualitative factors such as the place of deposit of the embryo (right or left horn) and embryo quality, were evaluated using th...

The aim of this study was to evaluate the main physical characteristics and correlations of fibre diameter (FD), coefficient of variation (CVFD), comfort factor (CF) and itching factor (IF) of alpaca fibre in the dry agroecological zone of the Puno region, Peru. In total, 14 068 fibre samples were collected and analysed with the OFDA 2000 equipment. The factors sex (male, female), breed (Suri, Huacaya), age (DL: milk teeth; 2 DL: two teeth; 4D: four teeth; BLL: full mouth) and province (Melgar, Chucuito, Puno, El Collao, Lampa, San Román) through a factorial arrangement of 2×2×4×6 was evaluated. The FD was influenced by age, sex, breed and province (p<0.01), being smaller in alpacas DL (20.10 μm) and thicker in BLL (23.29 μm). In males it was 21.25 μm and in females 21.91 μm (p<0.05) and in Huacaya alpacas 21.25 μm and in Suri 22.21 μm (p<0.05). The CVFD and IF variab...

The aim of this study was to evaluate two protocols for oestrus synchronization using intravaginal progestogens. In the study, 64 ewes with lambs at foot of 30-40 days old were used. The animals were assigned to two groups: (T1): application of a short synchronization protocol (6 days) with suppression of suckling for 30 hours (n1=32) and (T2): application of a long or conventional protocol (12 days) with suppression of suckling for 54 hours (n2=32). Fixed-time artificial insemination (FTAI) was performed with refrigerated semen through the transcervical route at the end of temporary weaning. The pregnancy rate without heat return at 16-17 days post-FTAI, pregnancy rate at 41 days by ultrasound, pregnancy rate at 90 days by balloting and type of delivery were determined. The rate of non-return to oestrus at 15-17 days post-FTAI was 81.25% for T1 and 87.50% for T2 (p<0.05). Likewise, t...

El objetivo del estudio fue evaluar dos protocolos para sincronizar el celo utilizando progestágenos intravaginales. En el estudio se utilizaron 64 ovejas con corderos al pie de 30-40 días. Los animales fueron asignados de dos grupos: (T1): aplicación de un protocolo de sincronización corto (6 días) con supresión del amamantamiento por 30 horas (n1=32) y (T2): aplicación de un protocolo largo o convencional (12 días) con supresión del amamantamiento por 54 horas (n2= 32). Se realizó inseminación artificial a tiempo fijo (IATF) con semen refrigerado por vía transcervical al término del destete temporal. Se determinó la tasa de preñez sin retorno de celo a los 16-17 días pos-IATF, tasa de preñez a 41 días por ecografía, tasa de preñez a los 90 días mediante balotaje y tipo de parto. La tasa de no retorno de celo a los 15-17 días pos-IATF fue de 81.25% para T1 y de 87....

El objetivo del trabajo fue evaluar el efecto de los protocolos corto (5 días) y largo (9 días) de SC en borregas inseminadas con semen congelado sobre el diámetro del cuerno uterino y tasa de preñez. El experimento se realizó en el Centro Experimental Carolina, perteneciente a la Universidad Nacional del Altiplano, y se utilizó 40 ovejas entre Corriedale y Criollas, en grupos de 20 para cada tratamiento, que fueron sincronizadas con esponjas intrava- ginales a base de Acetato de Medroxiprogesterona, durante 9 días (protocolo largo) y 5 días (protocolo corto) y al momento del retiro de la esponja se aplicó 350 UI de eCG. Para medir el diámetro del cuerno uterino se realizó la ecografía a las 36 h post retiro de la esponja y a las 56 h la inseminación cervical con semen descongelado y una posterior ecografía a los 35 días para confirmar la gestación. Los datos cuantitativo...

La investigación tuvo como objetivo evaluar un protocolo de inducción de celo en borregas lactantes con tratamiento hormonal de progestágenos y eCG con destete temporal de corderos para inseminación artificial a tiempo fijo por vía transcervical con semen refrigerado sobre la presentación de celo, preñez y natalidad. Se trabajó con 37 borregas distribuidas entre tres tratamientos: T1, borregas con destete temporal de la cría (n=15); T2, borregas con cría sin destete (n=10); T3, borregas en seca y sin cría (n=12). El destete temporal fue por 48 h previos a la inseminación. T1 y T3 presentaron una mayor tasa de celo manifiesto (100 y 91.66%, respectivamente) que T2 (60%; p<0.05). La tasa de no retorno de celo a los 14-17 días pos-servicio fue estadísticamente similar entre grupos (93.33, 60.0, 91.66% para T1, T2 y T3, respectivamente). En forma similar, el porcentaje de preñ...

La investigación tuvo como objetivo evaluar un protocolo de inducción de celo en borregas lactantes con tratamiento hormonal de progestágenos y eCG con destete temporal de corderos para inseminación artificial a tiempo fijo por vía transcervical con semen refrigerado sobre la presentación de celo, preñez y natalidad. Se trabajó con 37 borregas distribuidas entre tres tratamientos: T1, borregas con destete temporal de la cría (n=15); T2, borregas con cría sin destete (n=10); T3, borregas en seca y sin cría (n=12). El destete temporal fue por 48 h previos a la inseminación. T1 y T3 presentaron una mayor tasa de celo manifiesto (100 y 91.66%, respectivamente) que T2 (60%; p<0.05). La tasa de no retorno de celo a los 14-17 días pos-servicio fue estadísticamente similar entre grupos (93.33, 60.0, 91.66% para T1, T2 y T3, respectivamente). En forma similar, el porcentaje de pr...

The research aimed to evaluate a protocol of induction of oestrous in lactating ewes with hormonal treatment of progestins and eCG with temporary weaning of lambs for artificial insemination at a fixed time by transcervical route using refrigerated semen on the presentation of oestrous and pregnancy and lambing rate. In total, 37 ewes were distributed among three treatments: T1, lactating ewes with temporary weaning (n=15); T2, lactating ewes with lamb at foot (n=10); T3, dry ewes without lamb (n=12). Temporary weaning was for 48 h prior to insemination. T1 and T3 presented a higher rate of manifest oestrous (100 and 91.66%, respectively) than T2 (60%; p<0.05). The non-return heat rate at 14-17 days post-service was statistically similar between groups (93.33, 60.0, 91.66% for T1, T2 and T3, respectively). Similarly, the pregnancy percentage by ultrasound at 45 days and the percentage...

The viscous seminal plasma (SP) is currently a major impediment to the handling of ejaculate and the development of some biotechnologies in South American camelids. The vas deferens-collected spermatozoa of alpacas is a useful technique to avoid this problem. On the other hand, SP contains a large protein component that has been implicated in the function of spermatozoa within the female reproductive tract. In this sense, the low fertility achieved using transcervical insemination with frozen-thawed spermatozoa in alpacas could be improved by adding SP. This study aimed to evaluate the effect of the whole SP on some in vitro parameters of alpaca spermatozoa after the freezing-thawing-process and the fertility after artificial insemination. It would contribute to a better understanding of the interaction between thawed sperm cells and SP. Spermatozoa were obtained by surgically diverted v...