The aim of this study was to determine the factors that affect pregnancy in recipient females of embryos produced in vitro under high altitude conditions. The study was carried out at the Universidad Nacional del Altiplano (UNA), located in Puno, Peru, at an altitude of 3970 m. 50 recipient females (nulliparous, primiparous and multiparous) Brown Swiss were used. Ovaries were collected from cows at a local slaughterhouse and taken to the UNA animal reproduction laboratory, where the oocytes were collected, and in vitro maturation and fertilization, culture and embryo evaluation were carried out. The transport time to the laboratory was 2 to 3 h. Quantitative factors such as body condition score (CC), diameter of the corpus luteum (CL) and number of parities, and qualitative factors such as the place of deposit of the embryo (right or left horn) and embryo quality, were evaluated using th...

The present study was carried out in a laboratory of Animal Reproduction of the Faculty of Veterinary Medicine and Animal Science of the National University of the Puno-Peru Highland. The main objective was to evaluate the effect of in vitro and in vivo culture of alpacas zygotes produced in vitro. We used 226 cumulus oocyte complexes (CCOs), which were obtained from the ovaries from animals that were beneficiated in the camal and transported in 0.9% saline and supplemented with antibiotics. CCOs were aspirated from follicles of 2 to 6 mm. The oocytes were matured in TCM 2520, supplemented with 2.2 mg/mL with sodium bicarbonate, 0.0028 mg/mL sodium pyruvate, 10% fetal serum, 2 IU/mL equine chorionic gonadotropin, 10 IU/mL chorionic gonadotropin Human plus 50 ug/mL gentamicin and were cultured at 38.5°C, under 5% CO2, and high humidity for 36 h. Fertilization was...

The objective was to determine the addition of 5% carbon dioxide (CO2) from effervescent granules or gasified to a portable incubator (taper) to evaluate the in vitro production of embryos of oocytes aspirated from ovaries of cows slaughtered in the slaughterhouse and evaluate viability in vivo through pregnancy in recipient cows. As a portable incubator, a 500 cm3 plastic taper was used, to which a valve for controlling the addition of CO2 was conditioned. The maturation was carried out for 24 h, fertilization for 18 h and embryo culture for 7 d in 500 uL of the media in petry plates and introduced into the portable incubator, which was placed in a water bath at 38.5 ° C. At 72 h post fertilization the proportion of zygotes was determined, at 7 d post culture the embryos were counted. The fertilization results with the addition of CO2 from effervescent granules was 38.28...

The present study was carried out in a laboratory of Animal Reproduction of the Faculty of Veterinary Medicine and Animal Science of the National University of the Puno-Peru Highland. The main objective was to evaluate the effect of in vitro and in vivo culture of alpacas zygotes produced in vitro. We used 226 cumulus oocyte complexes (CCOs), which were obtained from the ovaries from animals that were beneficiated in the camal and transported in 0.9% saline and supplemented with antibiotics. CCOs were aspirated from follicles of 2 to 6 mm. The oocytes were matured in TCM 2520, supplemented with 2.2 mg/mL with sodium bicarbonate, 0.0028 mg/mL sodium pyruvate, 10% fetal serum, 2 IU/mL equine chorionic gonadotropin, 10 IU/mL chorionic gonadotropin Human plus 50 ug/mL gentamicin and were cultured at 38.5°C, under 5% CO2, and high humidity for 36 h. Fertilization was...

The objective was to determine the addition of 5% carbon dioxide (CO2) from effervescent granules or gasified to a portable incubator (taper) to evaluate the in vitro production of embryos of oocytes aspirated from ovaries of cows slaughtered in the slaughterhouse and evaluate viability in vivo through pregnancy in recipient cows. As a portable incubator, a 500 cm3 plastic taper was used, to which a valve for controlling the addition of CO2 was conditioned. The maturation was carried out for 24 h, fertilization for 18 h and embryo culture for 7 d in 500 uL of the media in petry plates and introduced into the portable incubator, which was placed in a water bath at 38.5 ° C. At 72 h post fertilization the proportion of zygotes was determined, at 7 d post culture the embryos were counted. The fertilization results with the addition of CO2 from effervescent granules was 38.28...

The present study was carried out in a laboratory of Animal Reproduction of the Faculty of Veterinary Medicine and Animal Science of the National University of the Puno-Peru Highland. The main objective was to evaluate the effect of in vitro and in vivo culture of alpacas zygotes produced in vitro. We used 226 cumulus oocyte complexes (CCOs), which were obtained from the ovaries from animals that were beneficiated in the camal and transported in 0.9% saline and supplemented with antibiotics. CCOs were aspirated from follicles of 2 to 6 mm. The oocytes were matured in TCM 2520, supplemented with 2.2 mg/mL with sodium bicarbonate, 0.0028 mg/mL sodium pyruvate, 10% fetal serum, 2 IU/mL equine chorionic gonadotropin, 10 IU/mL chorionic gonadotropin Human plus 50 ug/mL gentamicin and were cultured at 38.5°C, under 5% CO2, and high humidity for 36 h. Fertilization was...

The objective was to determine the addition of 5% carbon dioxide (CO2) from effervescent granules or gasified to a portable incubator (taper) to evaluate the in vitro production of embryos of oocytes aspirated from ovaries of cows slaughtered in the slaughterhouse and evaluate viability in vivo through pregnancy in recipient cows. As a portable incubator, a 500 cm3 plastic taper was used, to which a valve for controlling the addition of CO2 was conditioned. The maturation was carried out for 24 h, fertilization for 18 h and embryo culture for 7 d in 500 uL of the media in petry plates and introduced into the portable incubator, which was placed in a water bath at 38.5 ° C. At 72 h post fertilization the proportion of zygotes was determined, at 7 d post culture the embryos were counted. The fertilization results with the addition of CO2 from effervescent granules was 38.28...

The present study was carried out in a laboratory of Animal Reproduction of the Faculty of Veterinary Medicine and Animal Science of the National University of the Puno-Peru Highland. The main objective was to evaluate the effect of in vitro and in vivo culture of alpacas zygotes produced in vitro. We used 226 cumulus oocyte complexes (CCOs), which were obtained from the ovaries from animals that were beneficiated in the camal and transported in 0.9% saline and supplemented with antibiotics. CCOs were aspirated from follicles of 2 to 6 mm. The oocytes were matured in TCM 2520, supplemented with 2.2 mg/mL with sodium bicarbonate, 0.0028 mg/mL sodium pyruvate, 10% fetal serum, 2 IU/mL equine chorionic gonadotropin, 10 IU/mL chorionic gonadotropin Human plus 50 ug/mL gentamicin and were cultured at 38.5°C, under 5% CO2, and high humidity for 36 h. Fertilization was...

The objective was to determine the addition of 5% carbon dioxide (CO2) from effervescent granules or gasified to a portable incubator (taper) to evaluate the in vitro production of embryos of oocytes aspirated from ovaries of cows slaughtered in the slaughterhouse and evaluate viability in vivo through pregnancy in recipient cows. As a portable incubator, a 500 cm3 plastic taper was used, to which a valve for controlling the addition of CO2 was conditioned. The maturation was carried out for 24 h, fertilization for 18 h and embryo culture for 7 d in 500 uL of the media in petry plates and introduced into the portable incubator, which was placed in a water bath at 38.5 ° C. At 72 h post fertilization the proportion of zygotes was determined, at 7 d post culture the embryos were counted. The fertilization results with the addition of CO2 from effervescent granules was 38.28...

El objetivo del trabajo fue evaluar el efecto de los protocolos corto (5 días) y largo (9 días) de SC en borregas inseminadas con semen congelado sobre el diámetro del cuerno uterino y tasa de preñez. El experimento se realizó en el Centro Experimental Carolina, perteneciente a la Universidad Nacional del Altiplano, y se utilizó 40 ovejas entre Corriedale y Criollas, en grupos de 20 para cada tratamiento, que fueron sincronizadas con esponjas intrava- ginales a base de Acetato de Medroxiprogesterona, durante 9 días (protocolo largo) y 5 días (protocolo corto) y al momento del retiro de la esponja se aplicó 350 UI de eCG. Para medir el diámetro del cuerno uterino se realizó la ecografía a las 36 h post retiro de la esponja y a las 56 h la inseminación cervical con semen descongelado y una posterior ecografía a los 35 días para confirmar la gestación. Los datos cuantitativo...

El objetivo fue determinar las características ultrasonográfica B mode y Doppler del folículo dominante y cuerpo lúteo en vacas Criollas sometidas a un protocolo de sincronización de celo. El estudio fue realizado en la Estación Experimental Agraria ILLPA durante los meses de mayo a julio utilizando 15 vacas de raza Criolla con condición corporal regular y alimentadas con pasto natural, el protocolo consistió en colocar benzoato de estradiol y dispositivo intravaginal el primer día, posteriormente el día 7 se retiró el dispositivo y se aplicó un análogo de Prostaglandina F2α, el día 8 se aplicó nuevamente benzoato de estradiol y el día 9 se realizó la inseminación artificial con semen de un solo reproductor de raza Fleckvieh, la evaluación ultrasonográfica fue realizada los días 7 y 9 (iniciado la sincronización) para determinar el diámetro del folículo dominante...