The aim of this study was to evaluate the presence of the porcine circovirus 2 (PVC2) and the seroreactivity in pig farms in the regions of Tolima and Huila, Colombia, through a cross-sectional study. Blood and tissue samples (lymph nodes, lung and kidney) were taken from animals of all productive stages, including healthy animals and those with signs of the disease. The ELISA test detected seropositive animals in all productive stages. To identify the presence of the virus, the complete ORV2 of PCV2 was amplified by endpoint PCR using DNA extracted from blood, lymph nodes, lung and kidney. The samples were genotyped and finding positive animals for PCV2, both healthy and diseased, at all stages of production. Sequence analysis demonstrated a percentage of identity between 93 and 99% with the PCV2d genotype. Animals without positive clinical signs were positive for the ELISA test and for...

The aim of this study was to evaluate the presence of the porcine circovirus 2 (PVC2) and the seroreactivity in pig farms in the regions of Tolima and Huila, Colombia, through a cross-sectional study. Blood and tissue samples (lymph nodes, lung and kidney) were taken from animals of all productive stages, including healthy animals and those with signs of the disease. The ELISA test detected seropositive animals in all productive stages. To identify the presence of the virus, the complete ORV2 of PCV2 was amplified by endpoint PCR using DNA extracted from blood, lymph nodes, lung and kidney. The samples were genotyped and finding positive animals for PCV2, both healthy and diseased, at all stages of production. Sequence analysis demonstrated a percentage of identity between 93 and 99% with the PCV2d genotype. Animals without positive clinical signs were positive for the ELISA test and for...

The study was carried out with the in vitro embryo production of the In Vitro Colombia® laboratory of 2015. The embryo cleavage rate, the in vitro embryo production (IVP) and the pregnancy rate were analysed based on the type of semen used (sexed and conventional) for fertilization and on the bovine species (Bos taurus, Bos indicus). Data from 840 sessions of follicular aspiration were analysed, resulting in 127 503 oocytes placed in in vitro culture from 5257 females. The embryonic cleavage rate for sexed semen was 69.6% and for conventional semen 74.5% (p<0.001). It was obtained 20.8% of IVP for sexed semen and 26.5% for conventional semen (p<0.001). The sexed semen of B. taurus performed better in terms of embryonic cleavage than that of B. indicus (71.1 and 64.4%, respectively (p<0.001), whereas the conventional semen of B. indicus performed better than B. taurus (76.0 and ...

El estudio se realizó con la producción de embriones in vitro del laboratorio In Vitro Colombia® de 2015. Se analizó la tasa de división embrionaria, la producción de embriones in vitro (PIV) y la tasa de preñez teniendo en cuenta el tipo de semen utilizado (sexado y convencional) para la fertilización y la especie bovina (Bos taurus, Bos indicus). Se analizaron datos de 840 sesiones de aspiración folicular, resultando 127 503 oocitos puestos en cultivo in vitro provenientes de 5257 hembras. La tasa de división embrionaria para el semen sexado fue de 69.6% y para el semen convencional de 74.5% (p<0.001). Se obtuvo 20.8% de PIV para el semen sexado y de 26.5% para el convencional (p<0.001). El semen sexado de B. taurus se comportó mejor en términos de división embrionaria que el de B. indicus (71.1 vs. 64.4%) (p<0.001), mientras que el semen convencional de B. indicu...