The aim of this study was to evaluate the supplementation of Lactobacillus casei in sows and its effect on the zootechnical parameters of piglets. A total of 18 F1 Landrace x Large White sows were distributed in three completely randomized groups: control and two groups supplemented with L. casei (T1: 10 x 106 CFU [colony forming units]; T2: 10 x108 CFU). The doses were administered weekly orally 2 h after the first feeding of the day (09:00) for 180 days, covering a productive cycle. Results of zootechnical variables of each female and her piglets at the peripartum, at weaning and at the new service of the female were evauated. In the peripartum, a greater mean body weight of total births (PPNT) and average birth body weight were found (PPNV) in the supplemented animals compared to the control group (p<0.05). In the lactation phase there was a greater total weight per litter at weani...

The aim of this study was to evaluate the supplementation of Lactobacillus casei in sows and its effect on the zootechnical parameters of piglets. A total of 18 F1 Landrace x Large White sows were distributed in three completely randomized groups: control and two groups supplemented with L. casei (T1: 10 x 106 CFU [colony forming units]; T2: 10 x108 CFU). The doses were administered weekly orally 2 h after the first feeding of the day (09:00) for 180 days, covering a productive cycle. Results of zootechnical variables of each female and her piglets at the peripartum, at weaning and at the new service of the female were evauated. In the peripartum, a greater mean body weight of total births (PPNT) and average birth body weight were found (PPNV) in the supplemented animals compared to the control group (p<0.05). In the lactation phase there was a greater total weight per litter at weani...

A stable microbiota can act as an effective barrier against pathogen colonization besides to the production of metabolic substrates (e.g. vitamins, enzymes) and stimulate the mucosal immunity. Supplementation with probiotics have shown to improve the digestibility in pigs and work as an immunostimulant. The aim of this study was to assess the lipid metabolic profile in sows supplemented with Lactobacillus casei throughout a complete reproductive cycle. Eighteen F1 Landrace x Large White sows were randomly distributed in three experimental groups: control, T1 supplemented with 10 x 106 CFU (colony forming units)/g of L. casei and T2 supplemented with 10 x108 CFU/g of L. casei, administered weekly by oral route for 180 days, covering a reproductive cycle. Metabolites from lipid metabolic, hematic and protein profiles were analyzed in each sow at the day of service, 80-day and 113-day of pr...

The aim of this study was to describe a series of cases of hemoplasmosis in domestic cats in the city of Ibagué (Colombia), and the first report of Mycoplasma haemominutum in the country. Blood samples from patients admitted to the outpatient service of the Small Animal Clinic of the University of Tolima were analyzed by conventional PCR for the amplification of the 16s ribosomal gene to obtain a final product of 194 bb. In addition, hematic biometrics and serology were performed for viral diseases (Anigen FIV Ab/FeLV Ag Test kit). The amplicons obtained were subjected to sequencing and bioinformatic analysis (Geneious® 8.1.9). It was found hypochromic normocytic anemia in two cats, leukocytosis in a cat and thrombocytopenia in four of them. Two were seropositive for feline immunodeficiency virus antibodies and feline leukemia virus (FeLV) antigens and one was seropositive for FeLV. Sa...

The aim of this study was to evaluate the presence of the porcine circovirus 2 (PVC2) and the seroreactivity in pig farms in the regions of Tolima and Huila, Colombia, through a cross-sectional study. Blood and tissue samples (lymph nodes, lung and kidney) were taken from animals of all productive stages, including healthy animals and those with signs of the disease. The ELISA test detected seropositive animals in all productive stages. To identify the presence of the virus, the complete ORV2 of PCV2 was amplified by endpoint PCR using DNA extracted from blood, lymph nodes, lung and kidney. The samples were genotyped and finding positive animals for PCV2, both healthy and diseased, at all stages of production. Sequence analysis demonstrated a percentage of identity between 93 and 99% with the PCV2d genotype. Animals without positive clinical signs were positive for the ELISA test and for...

Una microbiota intestinal equilibrada constituye una barrera eficaz contra la colonización de patógenos, además de producir sustratos metabólicos (e.g. vitaminas, enzimas) y estimular el sistema inmunológico de mucosas. La suplementación con probióticos ha demostrado mejorar la digestibilidad en cerdos y servir como inmunoestimulante. El objetivo del presente trabajo fue evaluar el perfil metabólico lipídico en cerdas suplementadas con Lactobacillus casei a través de un ciclo reproductivo. Se emplearon 18 cerdas F1 Landrace x Large White distribuidas completamente al azar en tres grupos: control, T1 suplementado con 10 x 106 UFC (unidades formadoras de colonias/g) de L. casei y T2 suplementado con 10 x 108 UFC/g de L. casei, con frecuencia de administración semanal por vía oral, durante 180 días, cubriendo un ciclo reproductivo. Se analizaron los metabolitos del perfil lipí...

El objetivo del presente trabajo fue describir una serie de casos de hemoplasmosis en gatos domésticos en la ciudad de Ibagué (Colombia), y el primer reporte de Mycoplasma haemominutum en el país. Muestras de sangre de pacientes ingresados al servicio de consulta externa de la Clínica de Pequeños Animales de la Universidad del Tolima fueron analizadas por PCR punto final para la amplificación del gen 16s ribosomal a fin de obtener un producto final de 194 pb. Además, se hizo biometría hemática y serología para enfermedades virales (Anigen FIV Ab/FeLV Ag Test Kit). Los amplicones obtenidos fueron sometidos a secuenciamiento y análisis bioinformático (Geneious® 8.1.9). Se encontró anemia normocítica hipocrómica en dos gatos, leucocitosis en un gato y trombocitopenia en cuatro de ellos. Dos gatos resultaron serorreactivos para anticuerpos de virus de inmunodeficienca felina ...

The aim of this study was to evaluate the presence of the porcine circovirus 2 (PVC2) and the seroreactivity in pig farms in the regions of Tolima and Huila, Colombia, through a cross-sectional study. Blood and tissue samples (lymph nodes, lung and kidney) were taken from animals of all productive stages, including healthy animals and those with signs of the disease. The ELISA test detected seropositive animals in all productive stages. To identify the presence of the virus, the complete ORV2 of PCV2 was amplified by endpoint PCR using DNA extracted from blood, lymph nodes, lung and kidney. The samples were genotyped and finding positive animals for PCV2, both healthy and diseased, at all stages of production. Sequence analysis demonstrated a percentage of identity between 93 and 99% with the PCV2d genotype. Animals without positive clinical signs were positive for the ELISA test and for...

Eugenol (clove oil) has been widely used as an anaesthetic in fishes due to low induction and recovery times. The aim of this study was to assess the anaesthetic concentration of eugenol in 40 angel fish (Pterophyllum scalare) under laboratory conditions. The animals were exposed to four concentrations of eugenol (20, 30, 40 and 50 mg/L). Observations of the anaesthetics induction (phase I, II, and III) and recovery (phase I, II, III, and IV) were registered. The animals exposed to the higher concentrations of eugenol (40 and 50 mg/L) reached in a short time the first induction anaesthetic stage but had the longer recovery time (p<0.05). The 20 mg/L concentration of eugenol induce light anaesthetic stage in angel fish (phase I) while the 50 mg/L concentration induce an advanced stage of anaesthetic (III). The most effective concentration was 40 mg/L of eugenol for angel fish.

Eugenol (clove oil) has been widely used as an anaesthetic in fishes due to low induction and recovery times. The aim of this study was to assess the anaesthetic concentration of eugenol in 40 angel fish (Pterophyllum scalare) under laboratory conditions. The animals were exposed to four concentrations of eugenol (20, 30, 40 and 50 mg/L). Observations of the anaesthetics induction (phase I, II, and III) and recovery (phase I, II, III, and IV) were registered. The animals exposed to the higher concentrations of eugenol (40 and 50 mg/L) reached in a short time the first induction anaesthetic stage but had the longer recovery time (p<0.05). The 20 mg/L concentration of eugenol induce light anaesthetic stage in angel fish (phase I) while the 50 mg/L concentration induce an advanced stage of anaesthetic (III). The most effective concentration was 40 mg/L of eugenol for angel fish.