The species Leishmania infantum and L. chagasi are considered causal agents of visceral leishmaniasis, a relevant disease due to its zoonosis and severity in humans. Canines are the main reservoirs, hence the need to implement precise diagnoses to identify the Leishmania species involved. The aim of the study was to perform a molecular diagnosis of leishmaniasis (conventional PCR and Sanger sequencing) in 29 tissue samples from canines clinically suspected of leishmaniasis, previously diagnosed by non-molecular methods. The presence of the parasite could be identified by PCR in 9/29 samples (L. infantum, n=4; L. chagasi, n=4; L. braziliensis, n=1), evidencing a predominance of L. infantum and L. chagasi in canines with cutaneous and mucocutaneous leishmaniasis. The PCR allowed to clarify previously negative diagnoses; however, limitations were found, such as the absence of Leishmania DNA...

Una microbiota intestinal equilibrada constituye una barrera eficaz contra la colonización de patógenos, además de producir sustratos metabólicos (e.g. vitaminas, enzimas) y estimular el sistema inmunológico de mucosas. La suplementación con probióticos ha demostrado mejorar la digestibilidad en cerdos y servir como inmunoestimulante. El objetivo del presente trabajo fue evaluar el perfil metabólico lipídico en cerdas suplementadas con Lactobacillus casei a través de un ciclo reproductivo. Se emplearon 18 cerdas F1 Landrace x Large White distribuidas completamente al azar en tres grupos: control, T1 suplementado con 10 x 106 UFC (unidades formadoras de colonias/g) de L. casei y T2 suplementado con 10 x 108 UFC/g de L. casei, con frecuencia de administración semanal por vía oral, durante 180 días, cubriendo un ciclo reproductivo. Se analizaron los metabolitos del perfil lipí...

A stable microbiota can act as an effective barrier against pathogen colonization besides to the production of metabolic substrates (e.g. vitamins, enzymes) and stimulate the mucosal immunity. Supplementation with probiotics have shown to improve the digestibility in pigs and work as an immunostimulant. The aim of this study was to assess the lipid metabolic profile in sows supplemented with Lactobacillus casei throughout a complete reproductive cycle. Eighteen F1 Landrace x Large White sows were randomly distributed in three experimental groups: control, T1 supplemented with 10 x 106 CFU (colony forming units)/g of L. casei and T2 supplemented with 10 x108 CFU/g of L. casei, administered weekly by oral route for 180 days, covering a reproductive cycle. Metabolites from lipid metabolic, hematic and protein profiles were analyzed in each sow at the day of service, 80-day and 113-day of pr...