Introduction. Anisakidosis is a zoonosis caused by accidental ingestion of anisakid larvae (L3). Objective. To characterize the protein pattern and antigenic profile of the L3 of Anisakis simplex s.l. (type I), A. physeteris s.l. (type II) and Contracaecum osculatum s.l. commercial fish isolated. Methods. An SDS-PAGE electrophoretic run of the somatic antigens was performed. Rabbits were immunized experimentally and evaluated by EITB. Results. The protein pattern of Anisakis type I showed 12 bands, 18 Anisakis type II and C. osculatum 13, with bands 10 and 35 kDa specific for Anisakis type II, 28 and 77 for C. osculatum, notpresent in Anisakis type I. Conclusion. Specific immunogenic bands were determined for Anisakis type I with the molecular weight proteins 11, 14, 25 and 40 kDa, for type II of 9, 10, 12, 35 and 41 kDa and C. osculatum 13, 15, 17...

Introduction: Maytenus macrocarpa (MM) "Chuchuhuasi" is a native plant from the Amazon region of South America, widely used in traditional medicine due its anti-inflammatory, analgesic and anti-tumor properties. However, its effects on reproductive physiology and male fertility have not been elucidated. Methods: Preclinical experimental study of cases and controls.  Male mice with eight weeks from birth were administered orally for 70 days (two spermatogenic cycles) an aqueous extract MM: GC (n = 8) 0 mg / kg body weight (bw) control group and treated group GT (n = 8) 1000 mg / kg bw. Later the animals were euthanized, the reproductive organs were weighted, also motility and sperm count were recorded. During the days 60 or 65 of treatment, males were mated and pregnant females were sacrificed in the fourth day of pregnancy for evaluating development, quality and degree of blastulati...