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1
tesis de grado
Publicado 2012
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La actividad antibacteriana de muchos medicamentos actúa de forma bacteriostática o bactericida capaces de controlar a los diferentes agentes patógenos que causan enfermedades, pero a medida que van pasando los años y con el mal uso y abuso indiscriminado en la administración de los mismos, originan adherencia a que las bacterias mantengan cepas cada vez más resistentes. Actualmente, en la Amazonía Peruana se han reportado, por lo menos, 3140 especies útiles de la cuales aproximadamente 1044 especies tienen uso medicinal. El propósito del presente estudio fue determinar la actividad antibacteriana in vitro del tallo de Brosimun rubescens (Palisangre) para la cual la muestra en estudio fue recolectada en la zona de Reserva de Alpahuayo-Mishana e identificada taxonómicamente en el Herbarium Amazonense de la Universidad Nacional de la Amazonia Peruana y depositada en el laboratori...
2
artículo
In Peru, information on the biotechnological potential of native fungi for the degradation of cellulose presents few records. This work aimed to evaluate the cellulolytic activity of isolated fungi in the departments of Cajamarca, Lima, Junín and Huánuco. 289 species of fungi were subjected to reactivation after a conservation period of 7 years using filter paper and Czapeck broth, evaluated semi-quantitatively using the radial diffusion technique on Czapeck Na-CMC agar to then select five strains that were morphologically identified and its cellulolytic capacity was also determined by the Somogyi-Nelson method. 90% (260) of the fungi that underwent semi-quantitative evaluation were reactivated, selecting five strains for their greater clearance area: 14.9, 12.5, 12.0, 14.4 and 12.7 x 102 mm2; SA 726 (Paecilomyces sp), SA 668 (Paecilomyces sp), SA 651 (Paecilomyces sp), SA 683 (Fusariu...
3
tesis de grado
Publicado 2016
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El estudio corresponde al periodo 2015, con muestras recolectadas en la comunidad Nina-Rumí, provincia de Maynas, distrito de San Juan, departamento de Loreto, Perú. El propósito del presente estudio fue determinar la actividad antibacteriana In Vitro del extracto Etanólico de hojas de Plantago major (llantén), mediante los Métodos de Macrodilución y Difusión en Disco, la evaluación se realizó en los Laboratorios de la UNAP, mediante la metodología de Macrodilución en caldo y difusión de disco en agar frente a Staphylococcus aureus ATCC 25923 y Pseudomonas aeruginosa ATCC 27853. El extracto etanólico de hojas de Plantago major mostraron halos de inhibición de 20.3 mm y 20 mm a una concentración de 12 mg y 6 mg respectivamente, los resultados de la concentración mínima inhibitoria del extracto etanólico de hojas de Plantago major fueron de 1 mg/mL y de 4 mg/mL para Stap...
4
artículo
Publicado 2024
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El objetivo de la investigación fue determinar la resistencia antimicrobiana de aislados de Salmonella enterica de hisopados de cloaca de aves de pelea. Se evaluaron 135 muestras de aves de pelea provenientes de un galpón de crianza en el distrito de Carabayllo, Lima. Se identificaron tres aislados de Salmonella enterica representando el 2.2% (IC95%: 0-4.71%) del total de muestras. El enriquecimiento tardío en caldo Rappaport- Vassiliadis fue más eficiente en el aislamiento de la bacteria. Mediante la prueba de disco difusión con el método Kirby Bauer con 16 antibióticos se encontró que el 100% (3/3) de las aislados fueron resistentes a clindamicina, eritromicina, penicilina y estreptomicina, 66.7% (2/3) a oxitetracilina, ampicilina y doxiciclina. Esto representa una perspectiva preocupante, pues estos antibióticos son alternativas para la terapéutica contra este tipo de infecc...
5
artículo
Publicado 2025
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Objective: To evaluate the antimicrobial effect of plant extracts from native species of Moyobamba against clinically relevant pathogenic microorganisms. Material and Methods: Samples from five plant species were collected, and extracts were obtained through maceration with solvents of different polarities. A phytochemical analysis was performed to identify secondary metabolites, and antimicrobial activity was assessed using well diffusion and broth microdilution methods against Escherichia coli, Pseudomonas aeruginosa, Salmonella enterica, Staphylococcus aureus, and Klebsiella pneumoniae. Results: Only the bark extract of Indano (Byrsonima crassifolia) exhibited antimicrobial activity, inhibiting exclusively S. aureus with a minimum inhibitory concentration (MIC) of 0.195 mg/mL. Conclusions: The bark of B. crassifolia contains compounds with antibacterial potential against S. aureus, su...
6
artículo
Publicado 2023
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The aim of this study was to identify the antibiotic resistance of Salmonella enterica isolates from 384 samples of guinea pig carcasses from a market in Lima, Peru. The samples were inoculated in enrichment broth and cultured in specific media. Bacterial identification was performed using biochemical tests and antibiotic resistance using the plaque diffusion method against 16 antibiotics. Results showed that 28.9 ± 4.5% (111/384) of samples were positive for Salmonella enterica strains. Besides, 84.7% (94/111) showed resistance to at least one of the 16 antibiotics while 15.3% (17/111) were sensitive to all antibiotics. Likewise, 40.5% (45/111) of the strains showed multidrug resistance (MDR) to three or more antibiotics. The results confirm the risk that the commercialization of guinea pig carcasses contaminated with strains of Salmonella enterica resistant to antibiotics represents f...
7
artículo
Publicado 2020
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The aim of this study was to identify, serotype and determine the sensitivity profile of Salmonella enterica strains isolated from red-eared slider turtles (Trachemys sp) from two breeding centres in Lima, Peru, through cloacal swabs. The samples were isolated using tetrathionate enrichment broth, specific media such as Salmonella-Shigella agar and xylose lysine deoxycholate agar. The identification was made through biochemical tests. Serotyping through the recognition of somatic (O) and flagellar (H) antigens of the Kauffmann-White system, and the determination of sensitivity using the Kirby Bauer diffusion disk test. The results showed that 6.1% (4/66) of Salmonella enterica isolates were obtained, identifying the serotypes Salmonella Saintpaul and Salmonella Infantis. Antibacterial sensitivity tests indicated differences between strains, showing 100% sensitivity against nalidixic acid...
8
artículo
Publicado 2020
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The aim of this study was to identify, serotype and determine the sensitivity profile of Salmonella enterica strains isolated from red-eared slider turtles (Trachemys sp) from two breeding centres in Lima, Peru, through cloacal swabs. The samples were isolated using tetrathionate enrichment broth, specific media such as Salmonella-Shigella agar and xylose lysine deoxycholate agar. The identification was made through biochemical tests. Serotyping through the recognition of somatic (O) and flagellar (H) antigens of the Kauffmann-White system, and the determination of sensitivity using the Kirby Bauer diffusion disk test. The results showed that 6.1% (4/66) of Salmonella enterica isolates were obtained, identifying the serotypes Salmonella Saintpaul and Salmonella Infantis. Antibacterial sensitivity tests indicated differences between strains, showing 100% sensitivity against nalidixic acid...
9
artículo
Publicado 2020
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Introduction. The antibiotic resistance of Gram positive bacteria is a serious public health problem where some palliative measures can be found in the antimicrobial principles of filamentous fungi. Objective. To evaluate the antimicrobial activity of secondary metabolites of a clinical isolation of Aspergillus fumigatus on clinical strains of Staphylococcus aureus and Streptococcus pneumoniae. Methods. The liquid fermentation of A. fumigatus was carried out in a liquid broth sulfate, potato and dextrose; using ethyl acetate for the extraction of secondary metabolites. The antimicrobial activity considered as a halo greater than 6 mm was evaluated using the diffusion disk methodology. Results. A mean of 24,02 ± 2,51 mm and 23,62 ± 4,68 mm was obtained on sensitive and resistant Staphylococcus aureus, respectively. For Streptococcus pneumoniae sen...
10
artículo
Publicado 2016
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Los objetivos del presente estudio consistieron en aislar microorganismos de la piel de caninos con piodermitis de la ciudad de Santa Clara, Cuba, y evaluar la actividad in vitro de la furvina frente a los microorganismos aislados. La resistencia a los agentes antibacterianos se determinó mediante el método de difusión en agar y la actividad in vitro se evaluó cuantitativamente mediante macrodilución en caldo según procedimientos internacionales. Se identificaron 50 cepas multirresistentes, siendo 47 del género Staphylococcus y 3 del género Pseudomonas. El test exacto de Fisher demostró sensibilidad altamente significativa de los microorganismos del género Staphylococcus para la ciprofloxacina. Todas las cepas fueron resistentes al menos a tres de los antibacterianos enfrentados. Las muestras evaluadas mostraron una Concentración Inhibitoria Mínima (CIM) entre 2-16 g/ml fr...
11
artículo
Publicado 2016
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The aim of this study was to evaluate the in vitro activity of furvina against microorganisms isolated from the skin of canines with piodermatitis in of Santa Clara, Cuba. Resistance to antibacterial agents was assessed by the diffusion method in agar and the in vitro activity was evaluated quantitatively by the broth macrodilution method according to international clinical laboratory standards. Fifty multiresistant strains were identified, 47 from the genus Staphylococcus and 3 from Pseudomonas. The Fisher’s exact test showed highly significant sensibility of microorganisms of the genus Staphylococcus to ciprofloxacin. All strains were resistant to at least three antimicrobials. The Minimum Inhibitory Concentration (MIC) for furvina ranged from 2-16 mg/ml where 83.2% of the strains having a MIC of 4-8 mg/mL. The Minimum Bactericidal Concentration (MBC) ranged from 4-32 mg/ml where 86....
12
artículo
Publicado 2020
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Fishing activity residues contain high amounts of collagen and gelatin, which with enzymatic hydrolysis processes could manifest some biological activity. The present work aimed to determine the antioxidant and antimicrobial activities of gelatin hydrolysate obtained from Sarda chiliensis chiliensis “bonito” skin collagen. For this, collagen protein fractions were hydrolyzed with alcalase enzime at different conditions of pH, temperature, time and enzyme/ substrate ratio. The in vitro antioxidant activity was evaluated for the fifteen hydrolysates obtained, by 2,2-diphenyl-1-picrylhydracil radical (DPPH) and cationic radical 2,2-azinobis- 3-ethylbenzothiazolin-6-sulfonic (ABTS). The antimicrobial activity was evaluated by disk diffusion and broth microdilution for the minimum inhibitory concentration (MIC). Bonito gelatin hydrolyzates indicated hydrolysis degree above 10 % with a max...
13
artículo
Publicado 2011
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Propionibacterium acnes, Staphylococcus epidermidis and Staphylococcus aureus have been recognized as the bacteria that are involved in the inflammatory process of acne, while oxidants and antioxidants are involved in the repair of cutaneous tissue affected. In this study an evaluation was made of the antibacterial effect by the agar diffusion and broth dilution method, the cytoprotective and antioxidant effect on 3T3 dermic fibroblast cells, treated with hydrogen peroxide and the scavenging capacity of free radicals was determined by the 2, 2-diphenyl-l-picrylhydrazyl (DPPH) method as well as the Reducing Power of the ethanolic extracts of the leaves of the Machaerium floribundum. Minimal bactericidal concentrations (MBC) were obtained against Propionibacterium acnes and Staphylococcus aureus of 5 mg/mL and 2 mg/mL, respectively. A cytoprotective effect of 111% was observed over the cel...
14
artículo
Publicado 2011
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Propionibacterium acnes, Staphylococcus epidermidis and Staphylococcus aureus have been recognized as the bacteria that are involved in the inflammatory process of acne, while oxidants and antioxidants are involved in the repair of cutaneous tissue affected. In this study an evaluation was made of the antibacterial effect by the agar diffusion and broth dilution method, the cytoprotective and antioxidant effect on 3T3 dermic fibroblast cells, treated with hydrogen peroxide and the scavenging capacity of free radicals was determined by the 2, 2-diphenyl-l-picrylhydrazyl (DPPH) method as well as the Reducing Power of the ethanolic extracts of the leaves of the Machaerium floribundum. Minimal bactericidal concentrations (MBC) were obtained against Propionibacterium acnes and Staphylococcus aureus of 5 mg/mL and 2 mg/mL, respectively. A cytoprotective effect of 111% was observed over the cel...
15
artículo
Publicado 2015
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Introduction: Candida albicans may cause severe invasive infections and frequently superficial lesions. Drugs used in treatment present drawbacks and search of alternatives becomes necessary. Objectives: To determine the activity of Thymus vulgaris essential oil (EO) alone and combined with EDTA on Candida albicans, and formulate a cream with EO supplemented with natural terpenes. Design:In vitro experimental and in vivo toxicity study. Setting: Faculty of Medicine and Faculty of Pharmacy, Universidad Nacional Mayor de San Marcos, Lima, Peru. Biological material:Candida albicans strain for in vitro study and rabbits for dermal toxicity tests. Methods:Antifungal activity was determined by agar well diffusion method, and minimum inhibitory concentration (MIC) by broth microdilution method. A cream was formulated with EO 1% and another supplemented with carvacrol, thymol, citral and EDTA. D...
16
tesis de grado
Publicado 2019
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The objective of this study was to evaluate the antibiotic activity of the fractions of the crude extract of Rapidithrix thailandica s80 from Laguna Grande beach - Paracas. The bacterium was grown in liquid medium using marine broth, acetone was used as extraction solvent. The extract was concentrated under reduced pressure to dryness. Then a chromatographic fractionation was performed using Sephadex LH-20 as a stationary phase and methanol as the mobile phase. Three fractions were collected according to the presented color (light green, dark green and beisch). For the antibiotic activity, a concentration of 20 mg/mL was prepared, determined by the disk agar diffusion method and compared to methicillin-resistant Staphylococcus aureus (MRSA) ATCC 43300, Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Bacillus subtilis ATCC 6633 and Pseudomonas aeruginosa. The minimum inhibi...
17
tesis de grado
Publicado 2019
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El presente trabajo tuvo como objetivo evaluar y determinar la actividad antibacteriana in vitro del extracto etanólico de la corteza de Tynanthus panurensis (CLAVO HUASCA) frente a Staphylococcus aureus, Escherichia coli y Pseudomonas aeruginosa. La muestra vegetal fue recolectada del Centro Experimental de Plantas Medicinales (Jardín Botánico Arboterum) de la Facultad de Ciencias Agronómicas de la UNAP ubicado en la Carretera de Zungarococha; para la evaluación de la actividad antibacteriana in vitro se utilizó el método de Difusión en disco “Kirby-Bauer” y dilución en caldo “Macrodilución”, Se utilizaron como controles positivos los antibióticos meropenem, ciprofloxacino, gentamicina y vancomicina. Resultados: la actividad antibacteriana in vitro por el método de disco difusión, a concentraciones de 1300 mg/ml, 1500 mg/ml, y 1700 mg/ml del extracto etanólico de l...
18
tesis de grado
Publicado 2019
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The present investigation was to determine the in vitro effect of the ethanolic extract of the leaves of Annona muricata on the growth of Shigella boydii ATCC 9207. To this end, the ethanolic extract concentrations of 250 and 500 mg/mL were studied, it was evaluated by the diffusion method on Kirby Bauer agar, the bacteria was seeded on Mueller Hinton agar; we proceeded to make 6 mm diameter wells and 50 uL of each extract concentration was added; Ciprofloxacin was used as a positive control at a concentration of 5ug and as a negative control was used sterile distilled water, incubated at 37 ° C for 24 hours, 10 repetitions were performed, and the minimum inhibitory concentration (MIC) was determined by the macrodilution method in BHI broth. It was found that the average diameter of the inhibition halo for the concentration of 250mg / mL was 12.6mm and for 500mg / mL it was 16mm and acc...
19
tesis de grado
Publicado 2023
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En este estudio se evaluó la inhibición del crecimiento de Salmonella spp y Staphylococcus aureus expuestos a los extractos crudos de bacteriocinas producidos por bacterias ácido lácticas (BAL) aisladas de yogurt probiótico expendido en la ciudad de Cajamarca. Se obtuvo un total de 5 aislamientos que fueron caracterizados tanto bioquímica como molecularmente, se logró la identificación de Aerococcus viridans, Lacticaseibacillus rhamnosus, Lactobacillus paracasei y dos aislamientos de Streptococcus thermophilus. Se realizó la producción del extracto crudo de bacteriocinas de cada aislamiento en medio MRS broth, el cual fue incubado por 48 horas en un shaker orbital a 30 °C y 150 rpm, el sobrenadante fue centrifugado a 10000 rpm, se filtró con poro de 0,22 µm, fue sometido a estrés térmico a 80 °C y neutralizado con NaOH 1M; con la finalidad de inactivar el resto de compone...
20
artículo
Publicado 2018
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ABSTRACT Objective: To compare the in vitro antibacterial effect of samples of Apis mellifera honey produced in Costa, Sierra and Selva against Streptococcus mutans ATCC 25175. Material and methods: The effect was evaluated in 3 groups distributed in concentrations of 100%, 50 % and 25% for each honey. Five repetitions were made per experimental group. The bacterial sensitivity was evaluated by the well diffusion method. The bacterial specimen was reactivated in nutritious broth, to inoculate it in heart agar culture. Results: When facing the microorganism to the honeys of bee, average halos were obtained of inhibition of 27,84 mm and 14,38 mm for the concentrations of 100% and 50% of the honey of bee of origin of saw. Conclusion: nevertheless, bee honeys of coastal and jungle origin did not present inhibition halos. It is concluded that bee honey of Sierra origin at 100% and 50% have an...
