Introduction. The antibiotic resistance of Gram positive bacteria is a serious public health problem where some palliative measures can be found in the antimicrobial principles of filamentous fungi. Objective. To evaluate the antimicrobial activity of secondary metabolites of a clinical isolation of Aspergillus fumigatus on clinical strains of Staphylococcus aureus and Streptococcus pneumoniae. Methods. The liquid fermentation of A. fumigatus was carried out in a liquid broth sulfate, potato and dextrose; using ethyl acetate for the extraction of secondary metabolites. The antimicrobial activity considered as a halo greater than 6 mm was evaluated using the diffusion disk methodology. Results. A mean of 24,02 ± 2,51 mm and 23,62 ± 4,68 mm was obtained on sensitive and resistant Staphylococcus aureus, respectively. For Streptococcus pneumoniae sen...

La aspergilosis invasiva presenta alta mortalidad en pacientes con enfermedades crónicas e inmunocomprometidos. Aspergillus fumigatus sensu stricto (AFSS) es el principal agente etiológico y su tipificación requiere de métodos moleculares. La incidencia incrementada de AI resistentes a los antifúngicos demanda un diagnóstico certero y oportuno. Métodos. Se estudiaron 20 cepas de la micoteca del Instituto de Medicina Tropical – Universidad Nacional Mayor de San Marcos, aisladas de muestras respiratorias e identificadas como Aspergillus fumigatus sensu lato mediante el estudio macroscópico y microscópico. Las cepas fueron referidas a la Universidad Nacional del Litoral para su tipificación mediante una PCR screening para AFSS basada en secuencias del gen CYP51A y el estudio de sensibilidad antifúngica para voriconazol (VOR), itraconazol (ITC), posaconazol (POS), isavuconazol (...

Introduction. Tuberculosis is a public health problem in our country. Affected patients are slow to access health services, among other factors due to the stigma and social discrimination that this disease has in the population. In addition, stigma has an impact on the social and family relationship of affected patients. The objective of the present study was to quantify the stigma in students of the faculty of medicine of a public university. Methods. An observational, descriptive and transversal study was carried out. A previously validated questionnaire was applied to students of the first three years of medical school, in order to quantify the stigma of tuberculosis. Results. The questionnaire was applied to 505 students of the medical school. 39% of the student population showed a moderate and great stigma in front of the people affected by tuberculosis; likewise, 38% showed a sligh...

Objective: To determine the presence of Candida albicans in throat and nasal secretion in high school students. Design: Cross-sectional study. Setting: Institute of Tropical Medicine, Universidad Nacional Mayor de San Marcos, Lima, Peru. Participants: High school students from San Juan Macias School in Santa Anita, Lima, Peru. Interventions: Nasal and throat samples were collected from 102 14-17 year-old students. Samples were grown on sabouraud agar and Candida CHROMOagar and identified by chlamydospores study and metabolic tests. Main outcome measures: Identification of C. albicans yeast. Results: Candida yeast was isolated from 11 students (10.8%). A significant percentage of yeast (36.4%) developed moderate resistance to fluconazole. Conclusions: Continuous surveillance of medically important yeasts in nasopharyngeal carriers is suggested in order to be prepared for eventual infectio...