Tópicos Sugeridos dentro de su búsqueda.
Tópicos Sugeridos dentro de su búsqueda.
1
artículo
Publicado 2020
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Objective: to develop a microfluidic system (lab-on-a-chip) for detecting circulating breast cancer tumor cells. Materials and methods: the device was designed using 3D technology, and it was manufactures using soft photolithography and a laser cutting machine. The system performance and its magnetic settings were assessed using Jurkat cells and breast cancer cells that show different expression of CD45 and EpCAM surface markers. Antibodies against these markers were bound to magnetic pellets. Additionally, iron nanoparticles were used for assessing their entrapment. Results: nanoparticles were significantly trapped in the area set by magnetic field modeling. Tumor cells labeled with magnetic antibodies became trapped. Conclusions: we were able to manufacture a lab-on-a-chip system that is capable to trap circulating breast cancer tumor cells, which may become an excellent tool for diagn...
2
artículo
Publicado 2020
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Objective: to develop a microfluidic system (lab-on-a-chip) for detecting circulating breast cancer tumor cells. Materials and methods: the device was designed using 3D technology, and it was manufactures using soft photolithography and a laser cutting machine. The system performance and its magnetic settings were assessed using Jurkat cells and breast cancer cells that show different expression of CD45 and EpCAM surface markers. Antibodies against these markers were bound to magnetic pellets. Additionally, iron nanoparticles were used for assessing their entrapment. Results: nanoparticles were significantly trapped in the area set by magnetic field modeling. Tumor cells labeled with magnetic antibodies became trapped. Conclusions: we were able to manufacture a lab-on-a-chip system that is capable to trap circulating breast cancer tumor cells, which may become an excellent tool for diagn...
3
artículo
The large amount of molasses that are generated in sugar-processing companies are not always redistributed for commercialization in by-products. Because of this, the present research uses these wastes as fuel in low-cost, lab-scale, single-chamber microbial fuel cells. Zinc and copper electrodes were used as electrodes and 100 mL of molasse in the chamber as fuel, managing to generate current and voltage peaks of 1.73 ± 0.13 mA and 0.953 ± 0.142 V. In monitoring the conductivity of the substrate, a maximum peak of 111.156 ± 8.45 mS/cm was observed, and a slightly acidic pH was observed throughout the monitoring. It was possible to obtain a power density of 5.45 ± 0.31 W/cm2 for a current density of 308.06 mA/cm2, while the yeast count showed a logarithmic curve throughout the monitoring. Finally, the molecular technique identified 100% of the special C. boidinii present in the anodic...
4
artículo
Publicado 2010
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We have built an integrated system of sensors for organoleptic characterization of a liquor distilled from grapes called pisco analyzing its liquid phase in an electrochemical cell, in addition is analyzed its gas phase in a small chamber with the gas sensors. We have designed an electronic nose with 4 gas sensor with different characteristics (Figaro Inc.) and an electronic tongue based on an electrochemical cell with two electrodes of stainless steel with Pt and Au, respectively. The system is composed mainly of gas sensors, actuators valves, liquid and gas pumps that are governed by the P1C18F4523 microcontroller which also are responsible for sending data to the computer. The prototype developed is robust, economical and portable. For data processing we have developed a program in Matalab using principal components analysis. We have tested with different piscos obtaining interesting ...
5
artículo
Background: Cell-free DNA (cfDNA) is used in clinical research to identify biomarkers for diagnosis of and follow-up on cancer. Here, we propose a fast and innovative approach using traditional housekeeping genes as cfDNA targets in a copy number analysis. We focus on the application of highly sensitive technology such as digital PCR (dPCR) to differentiate breast cancer (BC) patients and controls by quantifying regions of PUM1 and RPPH1 (RNase P) in plasma samples. Methods: We conducted a case-control study with 82 BC patients and 82 healthy women. cfDNA was isolated from plasma using magnetic beads and quantified by spectrophotometry to estimate total cfDNA. Then, both PUM1 and RPPH1 genes were specifically quantified by dPCR. Data analysis was calibrated using a reference genomic DNA in different concentrations.Results: We found RNase P and PUM1 values were correlated in the patient g...
6
artículo
Publicado 2019
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Objective: Describe the chromosomal genotoxicity Eucalyptus globulus Labill meristems induced by caffeine andthe aberrations caused carbetamida in each mitotic phase. Methodology: Seeds sown in sterile Petri dishes with 20seeds per seven days of germination, the roots reach a length of one to two centimeters disinfected in bleach solution - distilled water (3:1). After germination treatments applied caffeine four hours and twelve of recovery, in thecase of one hour of treatment carbetamida no downtime. Results: We determined the mitotic process of Eucalyptusglobulus radical cells susceptible to caffeine and carbetamida. The research was carried out in selected rootlets ofseedlings with more vigor and growth in petri dishes 20 cm in diameter in the biology lab. The technique of crushingand staining with hydrochloric acetorceína mitotic analysis, studying an average of 1051.5 cells / plan...
7
artículo
Publicado 2021
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The authors acknowledge funding from the Federal Ministry of Education and Research (BMBF) for funding of the Young Investigator Group Perovskite Tandem Solar Cells within the program “Materialforschung für die Energiewende” (grant no. 03SF0540), the Helmholtz Association within the HySPRINT Innovation lab project, and the HyPerCells joint Graduate School. This research was supported by the joint agreement between the DAAD (German Academic Exchange Service) and FONDECYT (National Fund for Scientific, Technological Development and Technological Innovation) under the agreements 57508544 DAAD and 423-2019-FONDECYT. Further support has been provided by the PUCP vice chancellorship for research (VRI, project no. CAP-2019-3-0041/702). The authors thank Thomas Lußky for technical support, Bor Li for part of the sample preparation, and Norbert Koch, David Cahen, Isaac Balberg and Norbert N...
8
artículo
Publicado 2016
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Chilies are the main ingredients of the Peruvian gastronomy. Chili "Charapita" (Capsicum frutescens) is very used in amazon food. The last decade, "the gastronomic boom" has increased their demand, so it is necessary to develop new technologies for their preservation sustainably, healthy, without affecting the environment. The aim of the present work was to analyze the microbial population dynamics during Chili "Charapita" fermentation using spontaneous (SF) and controlled (CF) fermentation. Fermentations were conducted at pilot scale and room temperature with chilies from Iquitos city. A lactic acid bacteria (LAB) strain type belonging to Lactobacillus plantarum ATCC 14917 was used in FC. Fermentations were monitored through reducing sugars, acidity and pH determinations. Microbial tests were performed: total cells count by microscopy and microbial growth in culture media. LAB, yeasts, ...
9
artículo
Publicado 2016
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Chilies are the main ingredients of the Peruvian gastronomy. Chili "Charapita" (Capsicum frutescens) is very used in amazon food. The last decade, "the gastronomic boom" has increased their demand, so it is necessary to develop new technologies for their preservation sustainably, healthy, without affecting the environment. The aim of the present work was to analyze the microbial population dynamics during Chili "Charapita" fermentation using spontaneous (SF) and controlled (CF) fermentation. Fermentations were conducted at pilot scale and room temperature with chilies from Iquitos city. A lactic acid bacteria (LAB) strain type belonging to Lactobacillus plantarum ATCC 14917 was used in FC. Fermentations were monitored through reducing sugars, acidity and pH determinations. Microbial tests were performed: total cells count by microscopy and microbial growth in culture media. LAB, yeasts, ...
10
artículo
Publicado 2017
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Analysis of immune responses in Bartonella bacilliformis carriers are needed to understand acquisition of immunity to Carrion’s disease and may allow identifying biomarkers associated with bacterial infection and disease phases. Serum samples from 144 healthy subjects from 5 villages in the North of Peru collected in 2014 were analyzed. Four villages had a Carrion’s disease outbreak in 2013, and the other is a traditionally endemic area. Thirty cytokines, chemokines and growth factors were determined in sera by fluorescent bead-based quantitative suspension array technology, and analyzed in relation to available data on bacteremia quantified by RT-PCR, and IgM and IgG levels measured by ELISA against B. bacilliformis lysates. The presence of bacteremia was associated with low concentrations of HGF (p = 0.005), IL-15 (p = 0.002), IL-6 (p = 0.05), IP-10 (p = 0.008), MIG (p = 0.03) and ...
11
artículo
Publicado 2019
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Acidophilic microorganisms are predominant in the ecosystems of acid mine waters, being found in abundance the species Acidithiobacillus ferrooxidans. The objective of the study was to produce electricity in the Microbial Fuel Cells (MFC) from thiosulfate at the anode and ferric ion at the cathode using the bacteria At. ferrooxidans LB151, which was characterized morphologically and molecularly. The electrochemical techniques used were chronoamperometry and cyclic voltammetry. The cyclic voltammetry show current peaks of oxidation and reduction of the compounds indicating that there is an interaction of the bacteria with the surface of the electrode. The voltage generated by the MFC is greater than the control cell, producing an average power density of 24.23 mW/m2 in 4 hours while the control cell produced an average power density of 3.41 mW/m2 in the same time. The total energy generat...
12
tesis de grado
Publicado 2021
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Universidad Nacional Agraria La Molina. Facultad de Ciencias. Departamento Académico de Biología
13
artículo
Publicado 2018
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Polycystic liver disease without the presence of polycystic kidney disease is a rare clinical condition and its hemorrhagic complication is even rarer, with very few cases being reported. It can be life-threatening without a rapid intervention. Case report: A 40 year-old female who presented with moderate sharp right upper quadrant abdominal pain and nausea for one hour. Emergency department: blood pressure 130/80 mmHg, heart rate 100 beats per minute, temperature 37 °C, O2 saturation 99 %, respiratory rate 22 breaths per minute. On examination, soft and relaxed abdomen, right upper quadrant abdominal pain, no visceromegaly, positive Murphy’s sign and rebound sign. Labs: hematocrit 34 %, hemoglobin 11.4 g/dL, white blood cells 12190, amylase 70 U/L. Abdominal ultrasound: Polycystic liver disease with ruptured cyst, abundant free subcapsular fluid within cavity, without pancreas and ki...
14
artículo
Publicado 2022
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2,4 dinitrophenol (DNP) is a chemical that decouples oxidative phosphorylation leading to cell death. The case of an individual who ingested 2 grams of DNP in order to lose weight is presented. He was treated 22 hours after the exposure, with diaphoresis, agitation, sensation of fever and palpitations. He developed fever, tachycardia and tachypnea; and, in the lab tests, lactic acidosis, azoemia and leukocytosis with left deviation were evident. He evolved unfavorably with muscle stiffness, stridor and asystole, dying within 26 hours of ingestion of the poison. Management in these cases is supportive and gastrointestinal decontamination. There is no antidote.
15
artículo
Publicado 2015
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The egg is fertilized by a sperm cell and human, with an act of being, with a body and a souland therefore its first human desire is the right to live The advancement of medical and biological science, incorporates new factual situations that the law can not fail to understand as is the case with the consequences of the application of technical standards and assisted human reproduction techniques specifically in vitro fertilization which the procedure uses sperm and eggs needed to produce just one live embryo but several embryos be frozen without knowing what their fate generating the question, what happens to those human beings frozen in the lab? This problem requires urgent and appropriate legislation.
16
artículo
Low- and middle-income countries (LMICs) are significantly affected by SARS-CoV-2, partially due to their limited capacity for local production and implementation of molecular testing. Here, we provide detailed methods and validation of a molecular toolkit that can be readily produced and deployed using laboratory equipment available in LMICs. Our results show that lab-scale production of enzymes and nucleic acids can supply over 50,000 tests per production batch. The optimized one-step RT-PCR coupled to CRISPR-Cas12a-mediated detection showed a limit of detection of 102 ge/μL in a turnaround time of 2 h. The clinical validation indicated an overall sensitivity of 80%–88%, while for middle and high viral load samples (Cq ≤ 31) the sensitivity was 92%–100%. The specificity was 96%–100% regardless of viral load. Furthermore, we show that the toolkit can be used with the mobile lab...
17
tesis de grado
Publicado 2019
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El estudio se ejecutó en el Laboratorio de Anatomía y Tecnología de la Madera de la Facultad de Ciencias Forestales de la UNAP, Iquitos, con muestras de madera de las ramas de Nectandra acuminata “canela moena”, Pouroma tomentosa “sacha uvilla” y Tetrastylidium. peruvianum “huacapú negro”, obtenidas en el Jardín Botánico “El Huayo” (JBEH) del Centro de Investigación y Enseñanza Forestal Puerto Almendra. Se describió anatómicamente la estructura celular de la rama de las tres especies en base a las características cualitativas y cuantitativas de su estructura celular. El método utilizado fue el de Valderrama (2008). Los resultados muestran que la madera de la rama de N. acuminata y T. peruvianum tienen textura media y la de P. tomentosa tiene textura gruesa. N. acuminata y T. peruvianum presentan poros de diámetro mediano (136,78 μm y 150,15 μm, respectivame...
18
artículo
Publicado 2018
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This study is part of research developed in the area of Food Science, Department of Analytical Chemistry and Food, University of Vigo Ourense Campus, Spain.; whose ultimate goal is to occurring fermented beverages with probiotic properties with kefir grains juice of red and white grapes would thus not valuing vinificables grapes with low commercial value or direct consumption. And obtaining healthy drinks combining ink effects mainly polyphenol grape character with antioxidant kefir and functional benefits. Kinetics main fermentation process cash studied, the effect of change on the fermentation substrate and microbial populations analyzed kefir. The procedure was carried out as follows: the pellet was activated, conducted a sequence of four successive crops. In each one the juice grain was incubated of nonsterile red grape during 24 h. The grain separated and juice fermented by fr...
19
artículo
Publicado 2015
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Objective: To determine the enzymatic biochemical parameters (glutamic pyruvic transaminase (ALT), glutamic oxaloacetic transaminase (AST), alkaline phosphatase (ALP), gamma glutamyltransferase (y-GT), and lactate dehydrogenase (LDH)) in children with acute lymphoblastic leukemia (ALL) before cancer treatment. Material and Methods: A prospective experimental, observational, cross-sectional study was conducted in 30 children between 2 and 15 years old, from several Neoplastic Centers in Lima. Blood collection was performed in BD red cap Vacutainer tubes, processed in the semi-automated analyzer BIOTEC® EMP-168, with Wiener Lab Group enzyme reagents under the modified method Szaaz and UV-Optimized by IFCC, SSCC and SFBC. Finally, coding and tabulation was performed. Results: 60% were boys and 46.7% are between the ages of 2-6 years. Serum levels of AST were increased by 33.3% in boys and ...
20
tesis de grado
Publicado 2020
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La presente investigación tiene como finalidad el diseño, simulación y evaluación económica de un prototipo de sistema de refrigeración utilizando celdas Peltier para la conservación de la leche cruda para los pequeños comerciantes. Las pérdidas por deterioro de leche varían entre 10 y 20% mensual lo que sería para ellos una gran pérdida en términos de costos por no contar con un sistema de enfriamiento que pueda conservar la leche cruda durante más tiempo para su óptima comercialización. La investigación se dividió en cuatro etapas en donde se realizó en primer lugar el diseño del prototipo para enfriar 30 litros de leche, se definieron las medidas y material para luego ser dibujado en el programa Solidworks, a su vez se realizó un análisis de resistencia obteniéndose una deformación en la parte inferior de la base de 0.072 mm, así como una tensión máxima en p...
