La quimiotaxis es el movimiento de las células desde un gradiente de menor concentración a otro de mayor concentración de una sustancia soluble, está presente en la embriogénesis, diferenciación celular, regeneración de heridas y la metástasis de tumores. En la invasión celular se requiere que las células migren a través de la matriz extracelular, la cual es degradada por la metaloproteinasas de matriz, dirigiéndose luego a una nueva ubicación. Tanto las células normales como las tumorales pueden migrar en respuesta a estímulos inflamatorios. El objetivo de este proyecto es evaluar el hidrogel elaborado a partir de la matriz extracelular descelularizada del corazón de cerdo como modelo para evaluar la migración, invasión y quimiotaxis de la línea de cáncer de mama MCF-7. Para lo cual se descelularizó el corazón de cerdo para obtener una matriz extracelular rica en c...

Objective: to develop a microfluidic system (lab-on-a-chip) for detecting circulating breast cancer tumor cells. Materials and methods: the device was designed using 3D technology, and it was manufactures using soft photolithography and a laser cutting machine. The system performance and its magnetic settings were assessed using Jurkat cells and breast cancer cells that show different expression of CD45 and EpCAM surface markers. Antibodies against these markers were bound to magnetic pellets. Additionally, iron nanoparticles were used for assessing their entrapment. Results: nanoparticles were significantly trapped in the area set by magnetic field modeling. Tumor cells labeled with magnetic antibodies became trapped. Conclusions: we were able to manufacture a lab-on-a-chip system that is capable to trap circulating breast cancer tumor cells, which may become an excellent tool for diagn...

Objective: to develop a microfluidic system (lab-on-a-chip) for detecting circulating breast cancer tumor cells. Materials and methods: the device was designed using 3D technology, and it was manufactures using soft photolithography and a laser cutting machine. The system performance and its magnetic settings were assessed using Jurkat cells and breast cancer cells that show different expression of CD45 and EpCAM surface markers. Antibodies against these markers were bound to magnetic pellets. Additionally, iron nanoparticles were used for assessing their entrapment. Results: nanoparticles were significantly trapped in the area set by magnetic field modeling. Tumor cells labeled with magnetic antibodies became trapped. Conclusions: we were able to manufacture a lab-on-a-chip system that is capable to trap circulating breast cancer tumor cells, which may become an excellent tool for diagn...