Objetivos: Determinar la concentración de citocinas inflamatorias secretadas por Células Madre Mesenquimales (CMMs) derivadas del cordón umbilical Metodología: Estudio observacional, descriptivo, comparativo, transversal. Se cultivó CMM a partir de gelatina de Wharton o sangre de 13 cordones umbilicales obtenidos de partos atendidos en el Hospital Nacional Dos de Mayo (HNDM), de enero a diciembre de 2018. Se identificaron factores asociados al éxito del cultivo usando la prueba de chi cuadrado. Se determinó la concentración de citocinas pro inflamatorias secretadas por las CMM usando una prueba ELISA en el secretoma obtenido a partir de células extraídas de gelatina de Wharton. Todos los datos se tabularon y procesaron con el paquete estadístico STATA 10. Resultados: De los 17 cultivos celulares realizados, la tasa de éxito de crecimiento celular fue 33%. La gelatina de Whart...

Objective: to develop a microfluidic system (lab-on-a-chip) for detecting circulating breast cancer tumor cells. Materials and methods: the device was designed using 3D technology, and it was manufactures using soft photolithography and a laser cutting machine. The system performance and its magnetic settings were assessed using Jurkat cells and breast cancer cells that show different expression of CD45 and EpCAM surface markers. Antibodies against these markers were bound to magnetic pellets. Additionally, iron nanoparticles were used for assessing their entrapment. Results: nanoparticles were significantly trapped in the area set by magnetic field modeling. Tumor cells labeled with magnetic antibodies became trapped. Conclusions: we were able to manufacture a lab-on-a-chip system that is capable to trap circulating breast cancer tumor cells, which may become an excellent tool for diagn...

Objective: to develop a microfluidic system (lab-on-a-chip) for detecting circulating breast cancer tumor cells. Materials and methods: the device was designed using 3D technology, and it was manufactures using soft photolithography and a laser cutting machine. The system performance and its magnetic settings were assessed using Jurkat cells and breast cancer cells that show different expression of CD45 and EpCAM surface markers. Antibodies against these markers were bound to magnetic pellets. Additionally, iron nanoparticles were used for assessing their entrapment. Results: nanoparticles were significantly trapped in the area set by magnetic field modeling. Tumor cells labeled with magnetic antibodies became trapped. Conclusions: we were able to manufacture a lab-on-a-chip system that is capable to trap circulating breast cancer tumor cells, which may become an excellent tool for diagn...