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tesis de grado
Al hacer un análisis dialéctico, desde el punto de vista de la evolución de la ciencia, podemos apreciar que en este último decenio la medicina ha alcanzado grandes procesos con miras a buscar una sociedad más justa para el hombre. Es así que en la actualidad contamos con técnicas y métodos de diagnóstico cada vez más precisos, sin embargo aún existen muchos problemas por resolver. Uno de estos problemas son las afecciones del aparato urinario, especialmente las ocasionadas por bacterias, que de no realizarse un diagnóstico prematuro, la terapia a emplearse fracasará, debido a que las vías urinarias favorecen la instauración, la permanencia o la recidiva de la infección, siendo la principal manifestación la bacteriuria.
2
artículo
Publicado 2020
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Infectious coryza (IC), an upper respiratory tract disease affecting chickens, is caused by Avibacterium paragallinarum. The clinical manifestations of IC include nasal discharge, facial swelling, and lacrimation. This acute disease results in high morbidity and low mortality, while the course of the disease is prolonged and mortality rates are increased in cases with secondary infections. Studies regarding the immune response in infected chickens are scarce, and the local immune response is the focal point of investigation. However, a large body of work has demonstrated that severe infections can impact the systemic immune response. The objective of this study was to evaluate the systemic effects of Avibacterium paragallinarum (serovar B-1) infection on immune cells in specific pathogen-free (SPF) chickens. The current study revealed the presence of a transient circulating monocyte popu...
3
artículo
Publicado 2021
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It is important to have a PCR technique capable of detecting avian adenovirus 4 with high sensitivity and precision in cell cultures and samples of avian origin. In the present work, a PCR technique was standardized. First, the Internal Reference Material (IRM) was prepared, using cell culture in EB66 cells, infected with FAdV-4; then the virus was purified, the DNA was extracted and the amplicon was quantified. Finally, the temperature gradient test, primer concentration test, sensitivity test and specificity test were carried out using the Q5® High-Fidelity 2X Master PCR kit. Of the five primers used, the HHS*-3 and HHS*-4 primers passed all the tests, with HHS*-3 being the most sensitive as it detected up to 1 pg/µl.
4
artículo
Publicado 2021
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It is important to have a PCR technique capable of detecting avian adenovirus 4 with high sensitivity and precision in cell cultures and samples of avian origin. In the present work, a PCR technique was standardized. First, the Internal Reference Material (IRM) was prepared, using cell culture in EB66 cells, infected with FAdV-4; then the virus was purified, the DNA was extracted and the amplicon was quantified. Finally, the temperature gradient test, primer concentration test, sensitivity test and specificity test were carried out using the Q5® High-Fidelity 2X Master PCR kit. Of the five primers used, the HHS*-3 and HHS*-4 primers passed all the tests, with HHS*-3 being the most sensitive as it detected up to 1 pg/µl.
5
artículo
Publicado 2024
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The objective of the work was to optimize duplex PCR for the detection of Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS) in tracheal tissue samples in Gallus gallus in order to save time and economic resources in their diagnosis. The methodology consisted of carrying out an in silico evaluation of the ideal primers for their detection, the first tests such as primer concentration, sensitivity and specificity were carried out, followed by determining the prevalence in samples from different poultry houses at national level during a period of 4 years. Once the first tests were completed, the ideal primers (MG 3 and MS 2) were chosen for duplex PCR, achieving a sensitivity and specificity greater than 99%. This technique was put into practice and 669 samples of chicken tracheal tissue were analyzed, in which 45 samples were found to be positive for MG and/or MS. Finally, the pos...
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patente
Publicado 2022
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La presente invención se refiere a la obtención de una composición de gotas intranasales para prevenir la COVID-19 que tiene como componente principal unos anticuerpos IgY anti-RBDr de la proteína S del SARS-CoV-2. Además, un método para producir dicha composición
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artículo
Publicado 2018
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The objective of the study was to develop a molecular platform for the quantification of Newcastle disease virus (NDV) from a culture system in embryonated SPF eggs. First, four pairs of primers were evaluated that amplify different regions of the NDV viral genome that code for: nucleocapsid protein (NP), protein matrix (M), fusion protein (F) and RNA-dependent RNA polymerase (L) to select the most conserved one from which a molecular platform based on reverse transcription was developed - conventional polymerase chain reaction (RT-PCRc) in two steps for the detection of NDV. Subsequently, it was taken to reverse transcription - real-time polymerase chain reaction (RT-qPCR) for the quantification of NDV produced from a system of embryonated eggs. Through these techniques, it was determined that the primers for the M gene were adequate according to the optimization criteria for the develo...
8
artículo
Publicado 2018
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El estudio tuvo por objetivo desarrollar una plataforma molecular para la cuantificación del virus de la enfermedad de Newcastle (NDV) a partir de un sistema de cultivo en huevos embrionados SPF. Primero se evaluaron cuatro pares de cebadores que amplifican diferentes regiones del genoma viral de NDV que codifican para la proteína de nucleocápside (NP), proteína matriz (M), proteína fusión (F) y la ARN polimerasa ARN dependiente (L), con la finalidad de seleccionar el más conservado a partir del cual se desarrolló una plataforma molecular basada en la transcripción reversa - reacción en cadena de polimerasa convencional (RT-PCRc) en dos pasos para la detección del NDV. Posteriormente, esta fue llevada a transcripción reversa - reacción en cadena de polimerasa en tiempo real (RT-qPCR) para la cuantificación de NDV producido a partir de un sistema de huevos embrionados. Media...
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Publicado 2022
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Within the framework of the current COVID-19 pandemic, there is a race against time to find therapies for the outbreak to be controlled. Since vaccines are still tedious to develop and partially available for low-income countries, passive immunity based on egg-yolk antibodies (IgY) is presented as a suitable approach to preclude potential death of infected patients, based on its high specificity/avidity/production yield, cost-effective manufacture, and ease of administration. In the present study, IgY antibodies against a recombinant RBD protein of SARS-CoV-2 were produced in specific-pathogen-free chickens and purified from eggs using a biocompatible method. In vitro immunoreactivity was tested, finding high recognition and neutralization values. Safety was also demonstrated prior to efficacy evaluation, in which body weight, kinematics, and histopathological assessments of hamsters cha...
10
artículo
Publicado 2021
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El texto completo de este trabajo no está disponible en el Repositorio Académico UPC por restricciones de la casa editorial donde ha sido publicado.
